Development of Nanoparticle-based Platforms for Potential Applications in Biosensing and Therapeutics

Development of Nanoparticle-based Platforms for Potential Applications in Biosensing and Therapeutics

Bibliographic Details
Main Author: Wang, Peng
Language:English
Published: University of Cincinnati / OhioLINK 2017
Subjects:
Nanotechnology
upconversion nanoparticle
DNA detection
photodynamic therapy
hybrid photosensitizer
Online Access:http://rave.ohiolink.edu/etdc/view?acc_num=ucin151186771296011
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spelling ndltd-OhioLink-oai-etd.ohiolink.edu-ucin1511867712960112021-08-03T07:04:45Z Development of Nanoparticle-based Platforms for Potential Applications in Biosensing and Therapeutics Wang, Peng Nanotechnology upconversion nanoparticle DNA detection photodynamic therapy hybrid photosensitizer In this dissertation, I first developed and optimize an upconversion nanoparticles-based DNA detection scheme on different target DNA sequences, and then I explored the syntheses and characterizations of a nanomaterial-photosensitizer platform used for photodynamic therapy of cancer cells and bacteria in vitro. In the first project, a novel ligase-assisted signal-amplifiable DNA detection scheme is demonstrated based on luminescent resonance energy transfer between upconversion nanoparticles and the intercalating dye, SYBR Green I. Target DNA serves as a template for two DNA probes, one of them covalently attached to upconversion nanoparticles, to be joined into a long, hairpin-forming DNA by ligase. The number of the resulting DNA strand, which brings SYBR Green I close to the upconversion nanoparticles, is amplified through thermal cycling. The method was proven to display high sensitivity and specificity for DNA detection. Factors affecting the detection specificity and sensitivity, including ligation temperature, the amount of ligase, and the number of thermal cycles, have been investigated to optimize the performance of the detection method. Based on our result, the detection scheme can easily differentiate the BRAF V600E mutation from the wild-type sequence with a mutant-to-wild-type ratio of 1:1000. A detection limit of 1 fmole BRAF V600E mutation is achieved. While for the target sequence of EGFR T790M, the differentiate ratio is 1:100. The results show that 0.01 pmole of EGFR T790M mutant can be readily detected.In the second project, I report a hybrid singlet oxygen production system, where strong resonance coupling between silver nanoparticles and photosensitizing molecules results in exceptionally high singlet oxygen production under both visible light and near-infrared light excitation, even for the photosensitizing molecules without near-infrared absorption. Also, our results indicate that the hybrid photosensitizers display low cytotoxicity without light illumination yet highly enhanced photodynamic inhibition efficacy against Hela cells under a broad spectrum of light illuminations including the near-infrared light, which has great implication in photodynamic therapy of deep-tissue cancers. In addition, formulation of hybrid photosensitizers dispersed in PEG matrix demonstrates high bacterial killing efficacy against both S. epidermidis and P. acnes, suggesting it has immense potential for treatment of acne vulgaris without involving antibiotics. 2017 English text University of Cincinnati / OhioLINK http://rave.ohiolink.edu/etdc/view?acc_num=ucin151186771296011 http://rave.ohiolink.edu/etdc/view?acc_num=ucin151186771296011 unrestricted This thesis or dissertation is protected by copyright: some rights reserved. It is licensed for use under a Creative Commons license. Specific terms and permissions are available from this document's record in the OhioLINK ETD Center.
collection NDLTD
language English
sources NDLTD
topic Nanotechnology
upconversion nanoparticle
DNA detection
photodynamic therapy
hybrid photosensitizer
spellingShingle Nanotechnology
upconversion nanoparticle
DNA detection
photodynamic therapy
hybrid photosensitizer
Wang, Peng
Development of Nanoparticle-based Platforms for Potential Applications in Biosensing and Therapeutics
author Wang, Peng
author_facet Wang, Peng
author_sort Wang, Peng
title Development of Nanoparticle-based Platforms for Potential Applications in Biosensing and Therapeutics
title_short Development of Nanoparticle-based Platforms for Potential Applications in Biosensing and Therapeutics
title_full Development of Nanoparticle-based Platforms for Potential Applications in Biosensing and Therapeutics
title_fullStr Development of Nanoparticle-based Platforms for Potential Applications in Biosensing and Therapeutics
title_full_unstemmed Development of Nanoparticle-based Platforms for Potential Applications in Biosensing and Therapeutics
title_sort development of nanoparticle-based platforms for potential applications in biosensing and therapeutics
publisher University of Cincinnati / OhioLINK
publishDate 2017
url http://rave.ohiolink.edu/etdc/view?acc_num=ucin151186771296011
work_keys_str_mv AT wangpeng developmentofnanoparticlebasedplatformsforpotentialapplicationsinbiosensingandtherapeutics
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