Analysis of Chromatin Accessibility in Human Epidermis Identifies Putative Barrier Dysfunction-sensing Enhancers
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University of Cincinnati / OhioLINK
2017
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| Online Access: | http://rave.ohiolink.edu/etdc/view?acc_num=ucin1511800830009293 |
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ndltd-OhioLink-oai-etd.ohiolink.edu-ucin15118008300092932021-08-03T07:04:45Z Analysis of Chromatin Accessibility in Human Epidermis Identifies Putative Barrier Dysfunction-sensing Enhancers Lander, Julie M. Developmental Biology TSLP chromatin epidermis barrier transcription human To identify putative gene regulatory regions that respond to epidermal injury, we mapped chromatin dynamics in a stratified human epidermis during barrier maturation and disruption. Engineered skin substitutes (ESS) cultured at the air-liquid interface were used as a model of developing human epidermis with incomplete barrier formation. The epidermal barrier stabilized following engraftment onto immunocompromised mice, and was injured in vivo. Modified formaldehyde-assisted isolation of regulatory elements (FAIRE) was used to identify accessible genomic regions characteristic of monolayer keratinocytes, ESS in vitro, grafted ESS, and tape-stripped ESS graft. We mapped differentiation- and maturation-associated changes in transcription factor binding sites enriched at each stage and observed overrepresentation of AP-1 gene family motifs in barrier-deficient samples. Transcription of TSLP, an important effector of immunological memory in response to allergen exposure, was significantly elevated in our barrier-deficient samples. We identified dynamic DNA elements that correlated with TSLP induction and may contain enhancers that regulate TSLP. Two dynamic regions were located near the TSLP promoter and overlapped with allergy-associated SNPs rs17551370 and rs2289877, strongly implicating these loci in the regulation of TSLP expression in allergic disease. Additional dynamic chromatin regions ~250kb upstream of the TSLP promoter were found to be in high linkage disequilibrium with allergic disease SNPs. Taken together, these results define dynamic chromatin accessibility changes during epidermal development and barrier disruption. 2017 English text University of Cincinnati / OhioLINK http://rave.ohiolink.edu/etdc/view?acc_num=ucin1511800830009293 http://rave.ohiolink.edu/etdc/view?acc_num=ucin1511800830009293 unrestricted This thesis or dissertation is protected by copyright: some rights reserved. It is licensed for use under a Creative Commons license. Specific terms and permissions are available from this document's record in the OhioLINK ETD Center. |
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NDLTD |
| language |
English |
| sources |
NDLTD |
| topic |
Developmental Biology TSLP chromatin epidermis barrier transcription human |
| spellingShingle |
Developmental Biology TSLP chromatin epidermis barrier transcription human Lander, Julie M. Analysis of Chromatin Accessibility in Human Epidermis Identifies Putative Barrier Dysfunction-sensing Enhancers |
| author |
Lander, Julie M. |
| author_facet |
Lander, Julie M. |
| author_sort |
Lander, Julie M. |
| title |
Analysis of Chromatin Accessibility in Human Epidermis Identifies Putative Barrier Dysfunction-sensing Enhancers |
| title_short |
Analysis of Chromatin Accessibility in Human Epidermis Identifies Putative Barrier Dysfunction-sensing Enhancers |
| title_full |
Analysis of Chromatin Accessibility in Human Epidermis Identifies Putative Barrier Dysfunction-sensing Enhancers |
| title_fullStr |
Analysis of Chromatin Accessibility in Human Epidermis Identifies Putative Barrier Dysfunction-sensing Enhancers |
| title_full_unstemmed |
Analysis of Chromatin Accessibility in Human Epidermis Identifies Putative Barrier Dysfunction-sensing Enhancers |
| title_sort |
analysis of chromatin accessibility in human epidermis identifies putative barrier dysfunction-sensing enhancers |
| publisher |
University of Cincinnati / OhioLINK |
| publishDate |
2017 |
| url |
http://rave.ohiolink.edu/etdc/view?acc_num=ucin1511800830009293 |
| work_keys_str_mv |
AT landerjuliem analysisofchromatinaccessibilityinhumanepidermisidentifiesputativebarrierdysfunctionsensingenhancers |
| _version_ |
1719453212437643264 |