DNA-based Species Delimitation of the Agriculturally Important Genus, Ravinia (Diptera: Sarcophagidae)

Bibliographic Details
Main Author: Wong, Evan S.
Language:English
Published: University of Cincinnati / OhioLINK 2015
Subjects:
Online Access:http://rave.ohiolink.edu/etdc/view?acc_num=ucin1439561953
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spelling ndltd-OhioLink-oai-etd.ohiolink.edu-ucin14395619532021-08-03T06:33:01Z DNA-based Species Delimitation of the Agriculturally Important Genus, Ravinia (Diptera: Sarcophagidae) Wong, Evan S. Biology phylogenetics Chaetoravinia delineation cryptic species species-tree multilocus coalescent Species, and their constituent populations, are the units upon which evolution acts. The ability to accurately identify and delimit species boundaries is crucial for research, but is also important for public policy. Traditionally, species were identified and delimited based on morphological characteristics, yet sometimes species are cryptic, meaning no diagnostic morphological traits can be found. Consequently, DNA sequence data are increasingly being used in the areas of species delimitation and identification. As a model to explore species delimitation and identification I use members of the flesh fly family Sarcophagidae, specifically the genus Ravinia. Using Bayesian inference and maximum likelihood methods, I infer a molecular phylogeny, using mitochondrial DNA. Several paraphyletic relationships were inferred among species of Ravinia that are discordant with current morphological taxonomy. I investigate whether this conflict between genetic lineages and morphological identification within the species Ravinia anxia and Ravinia querula is due to introgressive gene flow, retained ancestral polymorphism, or indicative of the presence of unrecognized species. Using a combination of concatenated phylogenetic analyses, population genetic analyses, migration analyses, and coalescent methodologies I delimit the species Ravinia anxia and Ravinia querula. I find three morphologically cryptic lineages that comprise the current morphologically-identified species R. anxia and R. querula. With the findings of this species delimitation research, I then re-evaluate the phylogenetic relationships of Ravinia using increased sampling of both species and loci. 2015-10-12 English text University of Cincinnati / OhioLINK http://rave.ohiolink.edu/etdc/view?acc_num=ucin1439561953 http://rave.ohiolink.edu/etdc/view?acc_num=ucin1439561953 unrestricted This thesis or dissertation is protected by copyright: all rights reserved. It may not be copied or redistributed beyond the terms of applicable copyright laws.
collection NDLTD
language English
sources NDLTD
topic Biology
phylogenetics
Chaetoravinia
delineation
cryptic species
species-tree
multilocus coalescent
spellingShingle Biology
phylogenetics
Chaetoravinia
delineation
cryptic species
species-tree
multilocus coalescent
Wong, Evan S.
DNA-based Species Delimitation of the Agriculturally Important Genus, Ravinia (Diptera: Sarcophagidae)
author Wong, Evan S.
author_facet Wong, Evan S.
author_sort Wong, Evan S.
title DNA-based Species Delimitation of the Agriculturally Important Genus, Ravinia (Diptera: Sarcophagidae)
title_short DNA-based Species Delimitation of the Agriculturally Important Genus, Ravinia (Diptera: Sarcophagidae)
title_full DNA-based Species Delimitation of the Agriculturally Important Genus, Ravinia (Diptera: Sarcophagidae)
title_fullStr DNA-based Species Delimitation of the Agriculturally Important Genus, Ravinia (Diptera: Sarcophagidae)
title_full_unstemmed DNA-based Species Delimitation of the Agriculturally Important Genus, Ravinia (Diptera: Sarcophagidae)
title_sort dna-based species delimitation of the agriculturally important genus, ravinia (diptera: sarcophagidae)
publisher University of Cincinnati / OhioLINK
publishDate 2015
url http://rave.ohiolink.edu/etdc/view?acc_num=ucin1439561953
work_keys_str_mv AT wongevans dnabasedspeciesdelimitationoftheagriculturallyimportantgenusraviniadipterasarcophagidae
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