Anaerobic Biodegradation Patterns for Biodiesel
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University of Cincinnati / OhioLINK
2014
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| Online Access: | http://rave.ohiolink.edu/etdc/view?acc_num=ucin1417784154 |
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ndltd-OhioLink-oai-etd.ohiolink.edu-ucin14177841542021-08-03T06:28:36Z Anaerobic Biodegradation Patterns for Biodiesel Wu, Shuyun Environmental Engineering biodiesel biodegradation methanogenic petrodiesel FAMEs sulfate reducing Biodiesel, a mixture of Fatty Acid Methyl Esters (FAMEs) with or without petrodiesel, is considered a promising alternative liquid fuel and is gaining widespread acceptance. However, the impact and fate of biodiesel when accidentally released in the environment is poorly understood, especially under anaerobic conditions. Studies were designed to investigate the fate of biodiesel in an anaerobic environment and the impact of petrodiesel (petrodiesel) on the biodegradation of biodiesel.In the first set of experiments, biotransformation of soybean biodiesel and the inhibitory effect of petrodiesel were studied under methanogenic conditions. The kinetics of anaerobic biodegradation of soybean biodiesel B100 (signifying 100% biodiesel) blended with different petrodiesel loads were studied using biomass pre-acclimated to B100 (biodiesel only) and B80 (80% biodiesel and 20% petrodiesel). Results showed that soybean biodiesel was effectively degraded, whereas petrodiesel could not be degraded under methanogenic conditions. The presence of petrodiesel had a greater inhibitory effect on the rate of biodegradation than the biodegradation efficiency (defined as the methane production efficiency). Both the biodegradation rate coefficient and the methane production efficiency increased almost linearly with the increasing fraction of biodiesel.In a second set of experiments, biotransformation of soybean biodiesel (B100) and its biodiesel/petrodiesel blends were investigated under sulfate-reducing conditions. Three blends of biodiesel, B100, B50, and B0 were compared using cultures pre-acclimated to B100 and B80. The kinetics of biodegradation of individual FAMEs and the sulfate utilization rate were studied using a serum bottle reactor test. The biodegradation rate for saturated FAMEs decreased with an increase in the carbon chain length. For unsaturated FAMEs, the biodegradation rate increased with an increasing number of double bonds. In a third set of experiments, the kinetics of biodegradation of flaxseed biodiesel were tested under methanogenic and sulfate-reducing conditions to determine how changes in FAMEs profile affect the biodegradation of biodiesel. Under methanogenic conditions, the presence of moderate amounts of petrodiesel did not exhibit any inhibitory effects on the biodegradation rate of flaxseed biodiesel until the contribution of petrodiesel approached 50% by volume. Petrodiesel caused a greater inhibition effect on soybean biodiesel than flaxseed biodiesel with increasing petrodiesel loads. Under sulfate-reducing conditions, the same trend in biodegradation order occurred but the biodegradation rates were higher compared to soybean biodiesel. This trend was not related to the abundance profiles of FAMEs in the biodiesel, but the physiochemical properties of the individual FAME compounds. The abundance profiles of FAMEs in the biodiesel may affect their degradation rate due to inhibition effect of their hydrolysis products. 2014 English text University of Cincinnati / OhioLINK http://rave.ohiolink.edu/etdc/view?acc_num=ucin1417784154 http://rave.ohiolink.edu/etdc/view?acc_num=ucin1417784154 unrestricted This thesis or dissertation is protected by copyright: some rights reserved. It is licensed for use under a Creative Commons license. Specific terms and permissions are available from this document's record in the OhioLINK ETD Center. |
| collection |
NDLTD |
| language |
English |
| sources |
NDLTD |
| topic |
Environmental Engineering biodiesel biodegradation methanogenic petrodiesel FAMEs sulfate reducing |
| spellingShingle |
Environmental Engineering biodiesel biodegradation methanogenic petrodiesel FAMEs sulfate reducing Wu, Shuyun Anaerobic Biodegradation Patterns for Biodiesel |
| author |
Wu, Shuyun |
| author_facet |
Wu, Shuyun |
| author_sort |
Wu, Shuyun |
| title |
Anaerobic Biodegradation Patterns for Biodiesel |
| title_short |
Anaerobic Biodegradation Patterns for Biodiesel |
| title_full |
Anaerobic Biodegradation Patterns for Biodiesel |
| title_fullStr |
Anaerobic Biodegradation Patterns for Biodiesel |
| title_full_unstemmed |
Anaerobic Biodegradation Patterns for Biodiesel |
| title_sort |
anaerobic biodegradation patterns for biodiesel |
| publisher |
University of Cincinnati / OhioLINK |
| publishDate |
2014 |
| url |
http://rave.ohiolink.edu/etdc/view?acc_num=ucin1417784154 |
| work_keys_str_mv |
AT wushuyun anaerobicbiodegradationpatternsforbiodiesel |
| _version_ |
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