Qualitative Blood Coagulation Test Using Paper-Based Microfluidic Lateral Flow Device

Qualitative Blood Coagulation Test Using Paper-Based Microfluidic Lateral Flow Device

Bibliographic Details
Main Author: Li, Hua
Language:English
Published: University of Cincinnati / OhioLINK 2014
Subjects:
Engineering
Blood Coagulation
Paper-Based Device
Microfluidics
Lateral Flow
Point of Care
Online Access:http://rave.ohiolink.edu/etdc/view?acc_num=ucin1406810864
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spelling ndltd-OhioLink-oai-etd.ohiolink.edu-ucin14068108642021-08-03T06:26:29Z Qualitative Blood Coagulation Test Using Paper-Based Microfluidic Lateral Flow Device Li, Hua Engineering Blood Coagulation Paper-Based Device Microfluidics Lateral Flow Point of Care Research on paper-based microfluidic devices has grown into prosperity since 2007 when the Whitesides group in Harvard University first announced their work of qualitative bioassays on paper platform. These devices have a huge potential to be used in screening and point-of-care applications because they are easy to fabricate, simple to use, and low in cost. In this work, paper properties, wax printing, and micro-level fluid flow on paper-based materials are investigated first as preliminary study, and then a qualitative blood coagulation test device is developed utilizing traditional lateral flow platform.In order to utilize paper-based materials as test platform, various paper properties are studied to understand their behaviors with fluid present. When conducting different type of tests, different types of paper may be considered due to their unique properties. As a fabrication technique of paper device, wax printing is studied in detail to learn the printing accuracy and resolution of wax printer during device fabrication. By printing hydrophobic wax barriers on hydrophilic paper, wax printing is a very cheap, simple and fast way to fabricate microfluidic channels on paper device.Microfluidic flow behaviors of water and dextran aqueous solution with different viscosity are studied on cellulose papers and nitrocellulose membranes. Both fluids display the characteristics of Washburn flow on both types of materials. On cellulose material, fluid flows faster in narrower channels, which makes it possible to achieve flow control by manipulating channel geometry. On nitrocellulose material, fluid with smaller viscosity travels faster than that with larger viscosity, and the slope of (traveled distance)2 vs traveling time is linearly proportional to fluid viscosity on semi-log scale. This property makes it possible to anticipate fluid viscosity by measuring its traveling distance in a certain amount of time on nitrocellulose membrane.In the development of qualitative blood coagulation test device, traditional LFA structure consisting of sample pad, analytical membrane, and wick pad is utilized as diagnostic platform, and citrated rabbit blood and coagulant CaCl2 solution with different concentration are utilized to mimic human whole blood with various coagulation ability. Qualitative analysis of blood coagulation ability is performed by measuring the distance that different blood sample travels on analytical membrane. The further traveled distance indicates weaker coagulation ability, and the shorter traveled distance indicates stronger coagulation ability. A linear relationship between traveled distance and coagulation ability of blood sample is established, and whole blood coagulation ability can be anticipated by measuring its traveled distance on blood coagulation diagnostic LFA device in a certain amount of time. 2014-10-13 English text University of Cincinnati / OhioLINK http://rave.ohiolink.edu/etdc/view?acc_num=ucin1406810864 http://rave.ohiolink.edu/etdc/view?acc_num=ucin1406810864 unrestricted This thesis or dissertation is protected by copyright: all rights reserved. It may not be copied or redistributed beyond the terms of applicable copyright laws.
collection NDLTD
language English
sources NDLTD
topic Engineering
Blood Coagulation
Paper-Based Device
Microfluidics
Lateral Flow
Point of Care
spellingShingle Engineering
Blood Coagulation
Paper-Based Device
Microfluidics
Lateral Flow
Point of Care
Li, Hua
Qualitative Blood Coagulation Test Using Paper-Based Microfluidic Lateral Flow Device
author Li, Hua
author_facet Li, Hua
author_sort Li, Hua
title Qualitative Blood Coagulation Test Using Paper-Based Microfluidic Lateral Flow Device
title_short Qualitative Blood Coagulation Test Using Paper-Based Microfluidic Lateral Flow Device
title_full Qualitative Blood Coagulation Test Using Paper-Based Microfluidic Lateral Flow Device
title_fullStr Qualitative Blood Coagulation Test Using Paper-Based Microfluidic Lateral Flow Device
title_full_unstemmed Qualitative Blood Coagulation Test Using Paper-Based Microfluidic Lateral Flow Device
title_sort qualitative blood coagulation test using paper-based microfluidic lateral flow device
publisher University of Cincinnati / OhioLINK
publishDate 2014
url http://rave.ohiolink.edu/etdc/view?acc_num=ucin1406810864
work_keys_str_mv AT lihua qualitativebloodcoagulationtestusingpaperbasedmicrofluidiclateralflowdevice
_version_ 1719437028464001024

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