Influence of higher frequency components and duration of mechanical vibration on artery and bone in a rat-tail model
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University of Cincinnati / OhioLINK
2013
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ndltd-OhioLink-oai-etd.ohiolink.edu-ucin13781957122021-08-03T06:19:39Z Influence of higher frequency components and duration of mechanical vibration on artery and bone in a rat-tail model Goenka, Shilpi Engineering nitrotyrosine hand arm vibration rat-tail model vascular damage osteocyte bone Hand-Arm Vibration Syndrome (HAVS) is caused by hand-transmitted vibration in industrial workers and consists of vascular and musculoskeletal disorders. Current International Standard Organization (ISO) guidelines might underestimate vascular injury associated with range of vibration frequencies near resonance. Also, delineation of the response of bone tissue under different frequencies of vibration will allow formulation of more accurate guidelines for musculoskeletal disorders (MSD) linked to HAVS and eventually for its therapeutic outcome. A rat-tail model is used to investigate the effects of higher frequencies > 100 Hz on early vascular damage and bone changes. Two separate set of experiments were conducted to assess changes in artery tissue and bone under vibration. For experiment 1, 13 male Sprague Dawley rats (250 ± 15 gm) were used. Rat-tails were vibrated at 125 Hz and 250 Hz (49 m/s2) for 1D, 5D and 10D; D=days (4hr/day). Structural damage of vessels was quantified by vacuole count using Toluidine blue staining whereas biochemical changes were assessed by nitrotyrosine (NT) staining. The results were analyzed using one-way repeated measures mixed model ANOVA at p< 0.05 level of significance. For experiment 2, 24 Male Sprague Dawley rats (250 ± 15 gm) were used to investigate the effects of higher frequencies >100 Hz and duration of vibration (1D, 5D and 20D; D=days) on bone tissue. Rat-tails were vibrated at 125 Hz and 250 Hz (49 m/s2) for 1D, 5D and 20D (4hr/day). Structural damage of bone was quantified using histological staining. The biochemical changes were assessed by mineralization changes (material tests) and nitrotyrosine (NT) staining. The results were analyzed using one-way repeated measures mixed model ANOVA at p< 0.05 level of significance. For the artery tissue, the structural damage peaked at 125 Hz causing significant vacuoles (40.62 ± 9.8) (compared to control group (8.36 ± 2.49)) and reduced at 250 Hz (12.33 ± 2.98). However, the biochemical damage (NT signal) increased significantly for 125 Hz (143.35 ± 5.8) and for 250 Hz (155.8 ± 7.35) compared to the control group (101.7 ± 4.18). Our results demonstrate that vascular damage in the form of structural and biochemical disruption is significant at 125 Hz and 250 Hz. Hence the current ISO guidelines might underestimate vascular damage at frequencies > 100 Hz.For the bone, structural damage in cortical bone was significant at 250 Hz while the structural damage in the trabecular bone showed a moderate significance at 125 Hz and 250 Hz. The biochemical damage was significant at both the 125 Hz and 250 Hz vibration frequencies. Also, the structural damage was significant at 1D and 20D for the trabecular bone, while it was significant at 5D for cortical bone. Also, the biochemical damage was significant at all the time points of vibration (1D, 5D and 20D). Our results demonstrate that bone alterations in the form of structural and biochemical disruption in bone tissue are significant at 125 Hz and 250 Hz. The duration of vibration also has a significant effect. 2013-10-21 English text University of Cincinnati / OhioLINK http://rave.ohiolink.edu/etdc/view?acc_num=ucin1378195712 http://rave.ohiolink.edu/etdc/view?acc_num=ucin1378195712 unrestricted This thesis or dissertation is protected by copyright: all rights reserved. It may not be copied or redistributed beyond the terms of applicable copyright laws. |
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NDLTD |
| language |
English |
| sources |
NDLTD |
| topic |
Engineering nitrotyrosine hand arm vibration rat-tail model vascular damage osteocyte bone |
| spellingShingle |
Engineering nitrotyrosine hand arm vibration rat-tail model vascular damage osteocyte bone Goenka, Shilpi Influence of higher frequency components and duration of mechanical vibration on artery and bone in a rat-tail model |
| author |
Goenka, Shilpi |
| author_facet |
Goenka, Shilpi |
| author_sort |
Goenka, Shilpi |
| title |
Influence of higher frequency components and duration of mechanical vibration on artery and bone in a rat-tail model |
| title_short |
Influence of higher frequency components and duration of mechanical vibration on artery and bone in a rat-tail model |
| title_full |
Influence of higher frequency components and duration of mechanical vibration on artery and bone in a rat-tail model |
| title_fullStr |
Influence of higher frequency components and duration of mechanical vibration on artery and bone in a rat-tail model |
| title_full_unstemmed |
Influence of higher frequency components and duration of mechanical vibration on artery and bone in a rat-tail model |
| title_sort |
influence of higher frequency components and duration of mechanical vibration on artery and bone in a rat-tail model |
| publisher |
University of Cincinnati / OhioLINK |
| publishDate |
2013 |
| url |
http://rave.ohiolink.edu/etdc/view?acc_num=ucin1378195712 |
| work_keys_str_mv |
AT goenkashilpi influenceofhigherfrequencycomponentsanddurationofmechanicalvibrationonarteryandboneinarattailmodel |
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1719434831740272640 |