Thr roles of Twist1 and Tbx20 in endocardial cell proliferation, migration, and differentiation during endocardial cushion development

Thr roles of Twist1 and Tbx20 in endocardial cell proliferation, migration, and differentiation during endocardial cushion development

Bibliographic Details
Main Author: SHELTON, ELAINE L.
Language:English
Published: University of Cincinnati / OhioLINK 2008
Subjects:
Biomedical Research
Molecular Biology
Twist1
Tbx20
endocardial cushion
development
chicken
heart
Online Access:http://rave.ohiolink.edu/etdc/view?acc_num=ucin1212062811
id ndltd-OhioLink-oai-etd.ohiolink.edu-ucin1212062811
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spelling ndltd-OhioLink-oai-etd.ohiolink.edu-ucin12120628112021-08-03T06:12:38Z Thr roles of Twist1 and Tbx20 in endocardial cell proliferation, migration, and differentiation during endocardial cushion development SHELTON, ELAINE L. Biomedical Research Molecular Biology Twist1 Tbx20 endocardial cushion development chicken heart In the United States, congenital heart defects (CHD) are among the most common birth defects, occurring in approximately 1/100 live births. The majority of CHDs involve abnormal valvuloseptal development. Heart valvulogenesis is initiated by the formation of endocardial cushions, which will ultimately remodel into mature valve leaflets. While much is known about early events in valve development, the molecular mechanisms underlying the transition from endocardial cushion to remodeling valve leaflet remain relatively unknown. Endocardial cushions are composed of highly proliferative, migratory, undifferentiated, mesenchymal valve progenitor cells embedded in a loose extracellular matrix (ECM). The studies herein will demonstrate that robust expression of Twist1, a basic helix-loop-helix transcription factor, and Tbx20, a T-box transcription factor, in response to BMP2 signaling regulates cell proliferation, migration, and mesenchymal gene expression in endocardial cushions. In addition, these studies demonstrate that Twist1 can regulate Tbx20 expression in endocardial cushion cells. Taken together, these findings provide evidence that Twist1 acts upstream of Tbx20 and functions as a high-level regulatory protein in mesenchymal cell proliferation, migration, and differentiation in endocardial cushions. The studies presented in this dissertation identify molecular pathways that govern heart valve development and provide insight into potential therapeutic avenues for those afflicted with congenital valve defects or adult valve disease. 2008-08-22 English text University of Cincinnati / OhioLINK http://rave.ohiolink.edu/etdc/view?acc_num=ucin1212062811 http://rave.ohiolink.edu/etdc/view?acc_num=ucin1212062811 unrestricted This thesis or dissertation is protected by copyright: all rights reserved. It may not be copied or redistributed beyond the terms of applicable copyright laws.
collection NDLTD
language English
sources NDLTD
topic Biomedical Research
Molecular Biology
Twist1
Tbx20
endocardial cushion
development
chicken
heart
spellingShingle Biomedical Research
Molecular Biology
Twist1
Tbx20
endocardial cushion
development
chicken
heart
SHELTON, ELAINE L.
Thr roles of Twist1 and Tbx20 in endocardial cell proliferation, migration, and differentiation during endocardial cushion development
author SHELTON, ELAINE L.
author_facet SHELTON, ELAINE L.
author_sort SHELTON, ELAINE L.
title Thr roles of Twist1 and Tbx20 in endocardial cell proliferation, migration, and differentiation during endocardial cushion development
title_short Thr roles of Twist1 and Tbx20 in endocardial cell proliferation, migration, and differentiation during endocardial cushion development
title_full Thr roles of Twist1 and Tbx20 in endocardial cell proliferation, migration, and differentiation during endocardial cushion development
title_fullStr Thr roles of Twist1 and Tbx20 in endocardial cell proliferation, migration, and differentiation during endocardial cushion development
title_full_unstemmed Thr roles of Twist1 and Tbx20 in endocardial cell proliferation, migration, and differentiation during endocardial cushion development
title_sort thr roles of twist1 and tbx20 in endocardial cell proliferation, migration, and differentiation during endocardial cushion development
publisher University of Cincinnati / OhioLINK
publishDate 2008
url http://rave.ohiolink.edu/etdc/view?acc_num=ucin1212062811
work_keys_str_mv AT sheltonelainel thrrolesoftwist1andtbx20inendocardialcellproliferationmigrationanddifferentiationduringendocardialcushiondevelopment
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