Development of a Synthetic Vernix Equivalent, and Its Water Handling and Barrier Protective Properties in Comparison with Vernix Caseosa
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University of Cincinnati / OhioLINK
2006
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ndltd-OhioLink-oai-etd.ohiolink.edu-ucin11543934782021-08-03T06:11:26Z Development of a Synthetic Vernix Equivalent, and Its Water Handling and Barrier Protective Properties in Comparison with Vernix Caseosa Tansirikongkol, Anyarporn Vernix caseosa water-in-oil emulsion sorption isotherm chymotrypsin epidermal barrier <p>Preterm infants have a very immature epidermal barrier characterized by the absence of stratum corneum. Vernix caseosa is a biological material coating the term newborn infants. Its unique water handling properties may facilitate in the development or restoration of such compromised epidermal barrier. However, it is not feasible to topically apply vernix collected from one infant to another due to the risk of this infectious material. Therefore, in this study we have developed a barrier cream with similar water handling properties to native vernix. It was accomplished by the use of suitable emulsifier, water/lipid ratio and lipid composition. Basically, synthetic vernix formulation contained 20 % w/w oil phase of vernix-like lipids, 78% w/w water phase and 2% w/w emulsifiers. Its high water content with slow water release and its water vapor permeability may provide the moisturizing property and may facilitate epidermal barrier repair, respectively. The water content of vernix, vernix corneocytes and synthetic vernix formulation as a function of relative humidity of the environment was investigated. At lower humidity region, they released and absorbed small amount of water suggesting the presence of bound water. The GAB, FHH and D’Arcy-Watt models were fit to the water sorption data with r<sup>2</sup> > 0.99, therefore, they may be used as a model to describe the water content of the native vernix. Synthetic vernix formulation closely mimics native vernix during the desorption process. However, due to its presumed lack of internal structure, synthetic vernix with complete dryness can not regain its initial amount of water in the sorption process.</p><p>Additional role of vernix as a barrier to the loss/penetration of chymotrypsin , an enzyme required for barrier homeostasis and, occasionally, causing skin irritation, was investigated. Vernix did provide significant protection against chymotrypsin penetration. This protective property of vernix can be closely matched by synthetic vernix formulation and Desitin®, a commercial ointment for treating diaper dermatitis.</p><p>In summary, the overall work provides an initial step for the development of synthetic vernix formulation.</p> 2006-10-02 English text University of Cincinnati / OhioLINK http://rave.ohiolink.edu/etdc/view?acc_num=ucin1154393478 http://rave.ohiolink.edu/etdc/view?acc_num=ucin1154393478 unrestricted This thesis or dissertation is protected by copyright: all rights reserved. It may not be copied or redistributed beyond the terms of applicable copyright laws. |
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NDLTD |
| language |
English |
| sources |
NDLTD |
| topic |
Vernix caseosa water-in-oil emulsion sorption isotherm chymotrypsin epidermal barrier |
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Vernix caseosa water-in-oil emulsion sorption isotherm chymotrypsin epidermal barrier Tansirikongkol, Anyarporn Development of a Synthetic Vernix Equivalent, and Its Water Handling and Barrier Protective Properties in Comparison with Vernix Caseosa |
| author |
Tansirikongkol, Anyarporn |
| author_facet |
Tansirikongkol, Anyarporn |
| author_sort |
Tansirikongkol, Anyarporn |
| title |
Development of a Synthetic Vernix Equivalent, and Its Water Handling and Barrier Protective Properties in Comparison with Vernix Caseosa |
| title_short |
Development of a Synthetic Vernix Equivalent, and Its Water Handling and Barrier Protective Properties in Comparison with Vernix Caseosa |
| title_full |
Development of a Synthetic Vernix Equivalent, and Its Water Handling and Barrier Protective Properties in Comparison with Vernix Caseosa |
| title_fullStr |
Development of a Synthetic Vernix Equivalent, and Its Water Handling and Barrier Protective Properties in Comparison with Vernix Caseosa |
| title_full_unstemmed |
Development of a Synthetic Vernix Equivalent, and Its Water Handling and Barrier Protective Properties in Comparison with Vernix Caseosa |
| title_sort |
development of a synthetic vernix equivalent, and its water handling and barrier protective properties in comparison with vernix caseosa |
| publisher |
University of Cincinnati / OhioLINK |
| publishDate |
2006 |
| url |
http://rave.ohiolink.edu/etdc/view?acc_num=ucin1154393478 |
| work_keys_str_mv |
AT tansirikongkolanyarporn developmentofasyntheticvernixequivalentanditswaterhandlingandbarrierprotectivepropertiesincomparisonwithvernixcaseosa |
| _version_ |
1719432410573045760 |