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ndltd-OhioLink-oai-etd.ohiolink.edu-ucin11480753842021-08-03T06:11:11Z Fabrication and Chemical Modification of Polymer Microchip Devices Suitable for Mass Spectrometry Mecomber, Justin Sheldon Polymers have become the primary substrate for biological microelectromechanical systems. The focus of this work is to develop prototyping microfabrication techniques and photochemical modifications of thermoplastic microfluidic devices, which are essential in integrating high-throughput proteomics with mass spectrometry. Conventional computer numerical controlled (CNC)-milling has been used to generate a variety of aluminum masters suitable for the replication of polymer-based microfluidic devices. These microchips consist of high-aspect-ratio channel networks, injection ports, and capillary interfaces. Because these microchips are to be used for analytical separations common in lab-on-a-chip devices, several advanced machining techniques were developed and used to overcome existing limitations of a CNC-milling based approach. The approaches outlined illustrate that conventional CNC-milling using equipment commonly found in university machine shops is a viable alternative to other more expensive master generation processes. Molding masters can be used in conjunction with hot embossing to produce polymer microchip platforms suitable for analytical use. A study comparing the electrophoretic separation performance attainable from microchips molded by masters fabricated using conventional CNC machining techniques with commercial microchips, wire imprinted microchips and microchips from LIGA molding devices, is presented. An electrophoresis-based system using fluorescence microscopy for detection was used to determine the analytical utility of these microchips. The separation performance of CNC microchips was comparable to commercially available microchips as well as those fabricated from LIGA masters. This low-cost CNC prototyping approach provides a new entry point for researchers interested in thermoplastic microchips, and can accelerate the development of polymer-based lab-on-a-chip devices. A novel photochemical approach to topochemically modify the surface of a polymeric microfluidic network is presented. This approach results in the covalent modification of the thermoplastic surface, and variations in the chemical functionality of the azide allow for advances such as the modification of a microfluidic network surface charge or patterned scaffolding for site-specific attachment of biological molecules. The significance of this approach arises from a combination of the photochemically controlled surface reaction, the numerous polymeric substrates amenable to modification, and the ability to vary the functionality by simple synthetic variations to the base perfluorinated aromatic azide. 2006-07-17 English text University of Cincinnati / OhioLINK http://rave.ohiolink.edu/etdc/view?acc_num=ucin1148075384 http://rave.ohiolink.edu/etdc/view?acc_num=ucin1148075384 unrestricted This thesis or dissertation is protected by copyright: all rights reserved. It may not be copied or redistributed beyond the terms of applicable copyright laws.
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NDLTD
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English
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NDLTD
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| author |
Mecomber, Justin Sheldon
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| spellingShingle |
Mecomber, Justin Sheldon
Fabrication and Chemical Modification of Polymer Microchip Devices Suitable for Mass Spectrometry
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| author_facet |
Mecomber, Justin Sheldon
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| author_sort |
Mecomber, Justin Sheldon
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| title |
Fabrication and Chemical Modification of Polymer Microchip Devices Suitable for Mass Spectrometry
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| title_short |
Fabrication and Chemical Modification of Polymer Microchip Devices Suitable for Mass Spectrometry
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| title_full |
Fabrication and Chemical Modification of Polymer Microchip Devices Suitable for Mass Spectrometry
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| title_fullStr |
Fabrication and Chemical Modification of Polymer Microchip Devices Suitable for Mass Spectrometry
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| title_full_unstemmed |
Fabrication and Chemical Modification of Polymer Microchip Devices Suitable for Mass Spectrometry
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| title_sort |
fabrication and chemical modification of polymer microchip devices suitable for mass spectrometry
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University of Cincinnati / OhioLINK
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| publishDate |
2006
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http://rave.ohiolink.edu/etdc/view?acc_num=ucin1148075384
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AT mecomberjustinsheldon fabricationandchemicalmodificationofpolymermicrochipdevicessuitableformassspectrometry
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1719432354938748928
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