TRANSCRIPTIONAL SIGNATURES DURING THE DEVELOPMENT OF METAL-INDUCED ACUTE LUNG INJURY: ROLE OF SURFACTANT PROTEIN B
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University of Cincinnati / OhioLINK
2006
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| Online Access: | http://rave.ohiolink.edu/etdc/view?acc_num=ucin1147987059 |
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ndltd-OhioLink-oai-etd.ohiolink.edu-ucin11479870592021-08-03T06:11:11Z TRANSCRIPTIONAL SIGNATURES DURING THE DEVELOPMENT OF METAL-INDUCED ACUTE LUNG INJURY: ROLE OF SURFACTANT PROTEIN B VENDITTO, CARMEN Acute lung injury Surfactant protein B Metallothionein Acute lung injury is the rapid onset of lung dysfunction with many causes. One such cause is occupational and/or environmental exposure to respirable metal particulates. Surfactant protein B (SFTPB) is found in the alveoli of the lungs and is known to be a critical gene for normal lung function. Metallothionein-1 (MT1) is a protein that forms bonds with certain metal ions and has long been used as a biomarker for metal exposure. The overall goal of this study is to determine how nickel affects surfactant and induces acute lung injury. Three specific objectives were: 1) to generate dose-response (death) curves for various metals on mouse-lung-epithelial cells, 2) to measure the extent of Sftpb and Mt1 transcription, and 3) a transcriptome-wide scan of gene transcription using microarray analysis that can confirm the hypothesis that transcriptional control of select genes is critical for host defense or susceptibility to nickel induced acute lung injury. Nickel was determined to decrease SFTPB and increase MT1, and many other transcripts were also affected and analyzed to gain insight as to how acute lung injury manifests itself in host organisms. Some of these other transcripts include thioredoxin reductase, EGLN1 with prolyl 4-hydroxylase, HIF-1α, ubiquitin, and the receptor for advanced glycation end products. The relationships found between the expression of these genes suggest that transcriptional regulation of their proteins is a determining factor in whether organisms develop acute lung injury, and whether these organisms can then survive acute lung injury. 2006-07-13 English text University of Cincinnati / OhioLINK http://rave.ohiolink.edu/etdc/view?acc_num=ucin1147987059 http://rave.ohiolink.edu/etdc/view?acc_num=ucin1147987059 unrestricted This thesis or dissertation is protected by copyright: all rights reserved. It may not be copied or redistributed beyond the terms of applicable copyright laws. |
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NDLTD |
| language |
English |
| sources |
NDLTD |
| topic |
Acute lung injury Surfactant protein B Metallothionein |
| spellingShingle |
Acute lung injury Surfactant protein B Metallothionein VENDITTO, CARMEN TRANSCRIPTIONAL SIGNATURES DURING THE DEVELOPMENT OF METAL-INDUCED ACUTE LUNG INJURY: ROLE OF SURFACTANT PROTEIN B |
| author |
VENDITTO, CARMEN |
| author_facet |
VENDITTO, CARMEN |
| author_sort |
VENDITTO, CARMEN |
| title |
TRANSCRIPTIONAL SIGNATURES DURING THE DEVELOPMENT OF METAL-INDUCED ACUTE LUNG INJURY: ROLE OF SURFACTANT PROTEIN B |
| title_short |
TRANSCRIPTIONAL SIGNATURES DURING THE DEVELOPMENT OF METAL-INDUCED ACUTE LUNG INJURY: ROLE OF SURFACTANT PROTEIN B |
| title_full |
TRANSCRIPTIONAL SIGNATURES DURING THE DEVELOPMENT OF METAL-INDUCED ACUTE LUNG INJURY: ROLE OF SURFACTANT PROTEIN B |
| title_fullStr |
TRANSCRIPTIONAL SIGNATURES DURING THE DEVELOPMENT OF METAL-INDUCED ACUTE LUNG INJURY: ROLE OF SURFACTANT PROTEIN B |
| title_full_unstemmed |
TRANSCRIPTIONAL SIGNATURES DURING THE DEVELOPMENT OF METAL-INDUCED ACUTE LUNG INJURY: ROLE OF SURFACTANT PROTEIN B |
| title_sort |
transcriptional signatures during the development of metal-induced acute lung injury: role of surfactant protein b |
| publisher |
University of Cincinnati / OhioLINK |
| publishDate |
2006 |
| url |
http://rave.ohiolink.edu/etdc/view?acc_num=ucin1147987059 |
| work_keys_str_mv |
AT vendittocarmen transcriptionalsignaturesduringthedevelopmentofmetalinducedacutelunginjuryroleofsurfactantproteinb |
| _version_ |
1719432340910899200 |