IMAGING MEMBRANE PROTEINS USING ATOMIC FORCE MICROSCOPY TECHNIQUES

IMAGING MEMBRANE PROTEINS USING ATOMIC FORCE MICROSCOPY TECHNIQUES

Bibliographic Details
Main Author: LAU, JOAN M.
Language:English
Published: University of Cincinnati / OhioLINK 2002
Subjects:
atomic force microscopy
membrane proteins
lattice array particles
Online Access:http://rave.ohiolink.edu/etdc/view?acc_num=ucin1022192720
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spelling ndltd-OhioLink-oai-etd.ohiolink.edu-ucin10221927202021-08-03T06:08:34Z IMAGING MEMBRANE PROTEINS USING ATOMIC FORCE MICROSCOPY TECHNIQUES LAU, JOAN M. atomic force microscopy membrane proteins lattice array particles Thousands of different membrane proteins exist in the human body and reside in the membranes of cells. Structural information regarding these proteins can provide insight into the function of these proteins. Acquiring membrane protein structural information is difficult, especially when preparation techniques that are traditionally used for soluble proteins such as crystallizing and isolating proteins are not easily adapted for membrane proteins. The atomic force microscope (AFM) provides an alternative for imaging membrane proteins. The preparation required does not require crystallizing or isolating proteins, and can imagine membrane proteins in their native environment. Techniques are explored that can utilize the AFM for imaging proteins, including examining isolated cell membrane patches and membrane surfaces. One technique utilizes plasma membrane from Xenopus laevis oocytes as a model system to study membrane. A novel technique is developed to isolate oocyte membranes by bursting the oocyte and depositing its membrane on a flat mica substrate. The flat surface membrane preparation allows high-resolution AFM images to be obtained, revealing a novel structure of densely packed particles. These particles exhibit a regular, repeating pattern of a lattice-like array with orderly packing, and are thus termed “lattice-like array particles” (LAPs). The LAPs are orderly yet imperfectly packed, are located in depressed pools, occur with a low frequency on the oocyte membrane surface, and have not previously been seen using other isolation and imaging methods. Histogram analysis of the center-to-center distance between LAPs suggest their size to be about 44 nm in diameter, considerably larger than other reported size estimates of IMPs. These results indicate that LAPs represent a novel membrane particle organization, which merits further study. Future developments using this method or further studies to develop alternative membrane preparations may help elucidate membrane protein structure. 2002-09-24 English text University of Cincinnati / OhioLINK http://rave.ohiolink.edu/etdc/view?acc_num=ucin1022192720 http://rave.ohiolink.edu/etdc/view?acc_num=ucin1022192720 unrestricted This thesis or dissertation is protected by copyright: all rights reserved. It may not be copied or redistributed beyond the terms of applicable copyright laws.
collection NDLTD
language English
sources NDLTD
topic atomic force microscopy
membrane proteins
lattice array particles
spellingShingle atomic force microscopy
membrane proteins
lattice array particles
LAU, JOAN M.
IMAGING MEMBRANE PROTEINS USING ATOMIC FORCE MICROSCOPY TECHNIQUES
author LAU, JOAN M.
author_facet LAU, JOAN M.
author_sort LAU, JOAN M.
title IMAGING MEMBRANE PROTEINS USING ATOMIC FORCE MICROSCOPY TECHNIQUES
title_short IMAGING MEMBRANE PROTEINS USING ATOMIC FORCE MICROSCOPY TECHNIQUES
title_full IMAGING MEMBRANE PROTEINS USING ATOMIC FORCE MICROSCOPY TECHNIQUES
title_fullStr IMAGING MEMBRANE PROTEINS USING ATOMIC FORCE MICROSCOPY TECHNIQUES
title_full_unstemmed IMAGING MEMBRANE PROTEINS USING ATOMIC FORCE MICROSCOPY TECHNIQUES
title_sort imaging membrane proteins using atomic force microscopy techniques
publisher University of Cincinnati / OhioLINK
publishDate 2002
url http://rave.ohiolink.edu/etdc/view?acc_num=ucin1022192720
work_keys_str_mv AT laujoanm imagingmembraneproteinsusingatomicforcemicroscopytechniques
_version_ 1719431612333031424

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