Evaluation of Polycardanol as an Antifouling Coating: An Experimental and Theoretical Assessment of Protein-Polymer Interactions

Bibliographic Details
Main Author:	Patania, Michelle
Language:	English
Published:	University of Toledo / OhioLINK 2016
Subjects:	Chemical Engineering protein adhesion DLVO theory polycardanol antifouling
Online Access:	http://rave.ohiolink.edu/etdc/view?acc_num=toledo148132605001625

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spelling	ndltd-OhioLink-oai-etd.ohiolink.edu-toledo1481326050016252021-08-03T06:39:23Z Evaluation of Polycardanol as an Antifouling Coating: An Experimental and Theoretical Assessment of Protein-Polymer Interactions Patania, Michelle Chemical Engineering protein adhesion DLVO theory polycardanol antifouling Protein adhesion to medical devices can interfere with their proper function. Once a layer of proteins is attached, this makes it easier for bacteria to attach, which can result in infection. Additionally, when blood proteins adsorb to a biomaterial, this facilitates the clot forming process, which can cause failure of the medical device. For these reasons, it is important to understand protein interaction with surfaces and to develop biomaterial surfaces that resist protein adhesion.In this study, the antifouling properties of polycardanol surfaces toward proteins are investigated. Theoretical methods are useful to predict and compare antifouling properties of surfaces. This study applies the Derjaguin-Landau-Verwey-Overbeek (DLVO) theory to predict protein adhesion to various polycardanol surfaces and controls such as glass and polyvinyl chloride (PVC). The DLVO theory provides interaction energy profiles for a system, in this case, a protein and surface in an aqueous medium at biological conditions. The proteins used in this study are serum albumin and fibrinogen. Experimental data for protein adhesion to polycardanol and control surfaces is then obtained by use of protein kits. Additives to polycardanol, including a fluoropolymer and polyethylene glycol, will be used to modify polycardanol surface properties, and the effect on protein adhesion will be assessed.The DLVO theory suggested that more hydrophilic surfaces, or those that have a greater affinity for water, have a greater energy barrier to serum albumin adhesion. Application of the theory shows that glass and polycardanol containing PEG should show resistance to protein adhesion. The experimental protein kit results, however, suggest that a monolayer of protein attaches within minutes to all the surfaces studied, and no significant antifouling behavior is shown for the experiments in this study. It is recommended that polycardanol surfaces containing greater additive content be investigated. Additionally, accounting for other considerations such as surface roughness and hydration effects may help explain the experimental results. 2016 English text University of Toledo / OhioLINK http://rave.ohiolink.edu/etdc/view?acc_num=toledo148132605001625 http://rave.ohiolink.edu/etdc/view?acc_num=toledo148132605001625 unrestricted This thesis or dissertation is protected by copyright: all rights reserved. It may not be copied or redistributed beyond the terms of applicable copyright laws.
collection	NDLTD
language	English
sources	NDLTD
topic	Chemical Engineering protein adhesion DLVO theory polycardanol antifouling
spellingShingle	Chemical Engineering protein adhesion DLVO theory polycardanol antifouling Patania, Michelle Evaluation of Polycardanol as an Antifouling Coating: An Experimental and Theoretical Assessment of Protein-Polymer Interactions
author	Patania, Michelle
author_facet	Patania, Michelle
author_sort	Patania, Michelle
title	Evaluation of Polycardanol as an Antifouling Coating: An Experimental and Theoretical Assessment of Protein-Polymer Interactions
title_short	Evaluation of Polycardanol as an Antifouling Coating: An Experimental and Theoretical Assessment of Protein-Polymer Interactions
title_full	Evaluation of Polycardanol as an Antifouling Coating: An Experimental and Theoretical Assessment of Protein-Polymer Interactions
title_fullStr	Evaluation of Polycardanol as an Antifouling Coating: An Experimental and Theoretical Assessment of Protein-Polymer Interactions
title_full_unstemmed	Evaluation of Polycardanol as an Antifouling Coating: An Experimental and Theoretical Assessment of Protein-Polymer Interactions
title_sort	evaluation of polycardanol as an antifouling coating: an experimental and theoretical assessment of protein-polymer interactions
publisher	University of Toledo / OhioLINK
publishDate	2016
url	http://rave.ohiolink.edu/etdc/view?acc_num=toledo148132605001625
work_keys_str_mv	AT pataniamichelle evaluationofpolycardanolasanantifoulingcoatinganexperimentalandtheoreticalassessmentofproteinpolymerinteractions
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Evaluation of Polycardanol as an Antifouling Coating: An Experimental and Theoretical Assessment of Protein-Polymer Interactions

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