Fetal Developmental Anatomy of the Human Cardiovascular and Central Nervous Systems Using Lugol’s Iodine Staining and Micro-Computed Tomography
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Ohio University Honors Tutorial College / OhioLINK
2014
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| Online Access: | http://rave.ohiolink.edu/etdc/view?acc_num=ouhonors1398950897 |
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ndltd-OhioLink-oai-etd.ohiolink.edu-ouhonors13989508972021-08-03T06:24:36Z Fetal Developmental Anatomy of the Human Cardiovascular and Central Nervous Systems Using Lugol’s Iodine Staining and Micro-Computed Tomography Spaw, Alexandra J. Anatomy and Physiology Biomedical Research Developmental Biology Medical Imaging Radiology Scientific Imaging developmental anatomy Lugols iodine fetal anatomy birth defects anatomy education micro-CT scanning Iodine staining with Lugol’s solution is a fast-developing technique that is revolutionizing imaging studies, because it provides the opportunity to distinguish between soft tissues through soaking of the specimen followed by computed tomography (CT scanning). Earlier protocols that resulted in marked tissue shrinkage have been modified by treating specimens with a buffered sucrose solution prior to staining with isotonic Lugol’s, yielding excellent results. This technique was used to compare the cardiovascular and central nervous systems of a series of human fetal specimens ranging in age from six weeks to 28 weeks. For this study, four specimens were analyzed that were initially labeled six weeks, 10-12 weeks, 12-14 weeks, and 16 weeks; the justification for these ages were unknown, and new gestational ages were estimated using multiple measures resulting in new age estimates of 8.5 weeks, 12.5 weeks, 13 weeks, and 15 weeks. Specimens were microCT scanned prior to soaking in Lugol’s iodine and then rescanned after soaking to compare size-related parameters and soft-tissue resolution. Anatomical structures were analyzed and segmented using the computer program Avizo, providing the basis for the generation of labeled images, 3D reconstructions, 3D interactive PDFs, movies, and other forms of digital representations of the information provided in the CT scan data. Results include all representations which lead to a catalog of developing anatomy from the late embryonic stage well into the fetal stage of gestation. The specimens analyzed showed mostly normal anatomy for their respective ages and demonstrate fetal landmarks of the organ systems in focus (e.g., ductus arteriosus, foramen ovale, and spinal cords extending farther caudally than in neonates or adults). However, the youngest specimen (8.5 weeks) demonstrated a suspected anomaly which appears to be a schizencephaly, possibly revealing the cause of death. The catalog created provides students and educators with multiple forms of media to study the fetal anatomy of these two systems and allows professionals to better visualize and interact with the reconstructed versions of key organ systems. The study of both normal and teratological anatomy will lead to greater knowledge of any defects and could potentially lead to improved treatment and surgical plans. 2014-05-02 English text Ohio University Honors Tutorial College / OhioLINK http://rave.ohiolink.edu/etdc/view?acc_num=ouhonors1398950897 http://rave.ohiolink.edu/etdc/view?acc_num=ouhonors1398950897 unrestricted This thesis or dissertation is protected by copyright: all rights reserved. It may not be copied or redistributed beyond the terms of applicable copyright laws. |
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NDLTD |
| language |
English |
| sources |
NDLTD |
| topic |
Anatomy and Physiology Biomedical Research Developmental Biology Medical Imaging Radiology Scientific Imaging developmental anatomy Lugols iodine fetal anatomy birth defects anatomy education micro-CT scanning |
| spellingShingle |
Anatomy and Physiology Biomedical Research Developmental Biology Medical Imaging Radiology Scientific Imaging developmental anatomy Lugols iodine fetal anatomy birth defects anatomy education micro-CT scanning Spaw, Alexandra J. Fetal Developmental Anatomy of the Human Cardiovascular and Central Nervous Systems Using Lugol’s Iodine Staining and Micro-Computed Tomography |
| author |
Spaw, Alexandra J. |
| author_facet |
Spaw, Alexandra J. |
| author_sort |
Spaw, Alexandra J. |
| title |
Fetal Developmental Anatomy of the Human Cardiovascular and Central Nervous Systems Using Lugol’s Iodine Staining and Micro-Computed Tomography |
| title_short |
Fetal Developmental Anatomy of the Human Cardiovascular and Central Nervous Systems Using Lugol’s Iodine Staining and Micro-Computed Tomography |
| title_full |
Fetal Developmental Anatomy of the Human Cardiovascular and Central Nervous Systems Using Lugol’s Iodine Staining and Micro-Computed Tomography |
| title_fullStr |
Fetal Developmental Anatomy of the Human Cardiovascular and Central Nervous Systems Using Lugol’s Iodine Staining and Micro-Computed Tomography |
| title_full_unstemmed |
Fetal Developmental Anatomy of the Human Cardiovascular and Central Nervous Systems Using Lugol’s Iodine Staining and Micro-Computed Tomography |
| title_sort |
fetal developmental anatomy of the human cardiovascular and central nervous systems using lugol’s iodine staining and micro-computed tomography |
| publisher |
Ohio University Honors Tutorial College / OhioLINK |
| publishDate |
2014 |
| url |
http://rave.ohiolink.edu/etdc/view?acc_num=ouhonors1398950897 |
| work_keys_str_mv |
AT spawalexandraj fetaldevelopmentalanatomyofthehumancardiovascularandcentralnervoussystemsusinglugolx2019siodinestainingandmicrocomputedtomography |
| _version_ |
1719436563240189952 |