Analyzing Algal Diversity in Aquatic Systems Using Next Generation Sequencing
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Ohio University Honors Tutorial College / OhioLINK
2013
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| Online Access: | http://rave.ohiolink.edu/etdc/view?acc_num=ouhonors1366807717 |
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ndltd-OhioLink-oai-etd.ohiolink.edu-ouhonors13668077172021-08-03T05:22:45Z Analyzing Algal Diversity in Aquatic Systems Using Next Generation Sequencing Thrush, Mariah A. Environmental Science Freshwater Ecology Genetics Plant Biology Algae can be broadly defined as photosynthetic, oxygen-producing aquatic bacteria or protists, with phytoplankton living in the open water and periphyton/benthic algae living on the stream bottom (benthos). High biodiversity within a stream is important; research has found that having many taxa within an aquatic ecosystem help keep streams healthy and functioning. Diversity of algae is typically analyzed and enumerated using microscopy, but molecular techniques have become a useful tool for researchers. Next generation sequencing using DNA barcodes is replacing Sanger sequencing due to the ability to assess all species in a single sample with a single reaction. Therefore, this thesis seeks to develop methodologies for conducting biodiversity studies using next generation sequencing within two different freshwater habitats as well as utilizing this information to assess stream health. Phytoplankton biodiversity of two samples from the Ohio and Muskingum Rivers were examined using DNA barcodes with next generation sequencing. Utilizing the methods developed for phytoplankton, an acid mine drainage remediation gradient along the length of Hewett Fork, a small stream in southeastern Ohio, was assessed with eight periphyton samples. With the phytoplankton study, it was found that next generation sequencing has great potential to become a powerful tool for analyzing algal diversity, but more algal sequences need to be uploaded to genomic databases to provide an accurate analysis of the algal community composition. Due to technical difficulties with new next generation sequencing chemistry, sequences for the acid mine drainage restoration gradient have not been generated. 2013-06-03 English text Ohio University Honors Tutorial College / OhioLINK http://rave.ohiolink.edu/etdc/view?acc_num=ouhonors1366807717 http://rave.ohiolink.edu/etdc/view?acc_num=ouhonors1366807717 unrestricted This thesis or dissertation is protected by copyright: all rights reserved. It may not be copied or redistributed beyond the terms of applicable copyright laws. |
| collection |
NDLTD |
| language |
English |
| sources |
NDLTD |
| topic |
Environmental Science Freshwater Ecology Genetics Plant Biology |
| spellingShingle |
Environmental Science Freshwater Ecology Genetics Plant Biology Thrush, Mariah A. Analyzing Algal Diversity in Aquatic Systems Using Next Generation Sequencing |
| author |
Thrush, Mariah A. |
| author_facet |
Thrush, Mariah A. |
| author_sort |
Thrush, Mariah A. |
| title |
Analyzing Algal Diversity in Aquatic Systems Using Next Generation Sequencing |
| title_short |
Analyzing Algal Diversity in Aquatic Systems Using Next Generation Sequencing |
| title_full |
Analyzing Algal Diversity in Aquatic Systems Using Next Generation Sequencing |
| title_fullStr |
Analyzing Algal Diversity in Aquatic Systems Using Next Generation Sequencing |
| title_full_unstemmed |
Analyzing Algal Diversity in Aquatic Systems Using Next Generation Sequencing |
| title_sort |
analyzing algal diversity in aquatic systems using next generation sequencing |
| publisher |
Ohio University Honors Tutorial College / OhioLINK |
| publishDate |
2013 |
| url |
http://rave.ohiolink.edu/etdc/view?acc_num=ouhonors1366807717 |
| work_keys_str_mv |
AT thrushmariaha analyzingalgaldiversityinaquaticsystemsusingnextgenerationsequencing |
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