Effect of day of hatch inoculation with Enterobacteriaceae on inflammation and enteric permeability in broilers

Effect of day of hatch inoculation with Enterobacteriaceae on inflammation and enteric permeability in broilers

Bibliographic Details
Main Author: Chasser, Kaylin M.
Language:English
Published: The Ohio State University / OhioLINK 2021
Subjects:
Animal Sciences
Enterobacteriaceae
inflammation
alpha-1-glycoprotein
yolk sac retention
fluorescein isothiocyanate dextran
broiler
Online Access:http://rave.ohiolink.edu/etdc/view?acc_num=osu1618943283197541
id ndltd-OhioLink-oai-etd.ohiolink.edu-osu1618943283197541
record_format oai_dc
collection NDLTD
language English
sources NDLTD
topic Animal Sciences
Enterobacteriaceae
inflammation
alpha-1-glycoprotein
yolk sac retention
fluorescein isothiocyanate dextran
broiler
spellingShingle Animal Sciences
Enterobacteriaceae
inflammation
alpha-1-glycoprotein
yolk sac retention
fluorescein isothiocyanate dextran
broiler
Chasser, Kaylin M.
Effect of day of hatch inoculation with Enterobacteriaceae on inflammation and enteric permeability in broilers
author Chasser, Kaylin M.
author_facet Chasser, Kaylin M.
author_sort Chasser, Kaylin M.
title Effect of day of hatch inoculation with Enterobacteriaceae on inflammation and enteric permeability in broilers
title_short Effect of day of hatch inoculation with Enterobacteriaceae on inflammation and enteric permeability in broilers
title_full Effect of day of hatch inoculation with Enterobacteriaceae on inflammation and enteric permeability in broilers
title_fullStr Effect of day of hatch inoculation with Enterobacteriaceae on inflammation and enteric permeability in broilers
title_full_unstemmed Effect of day of hatch inoculation with Enterobacteriaceae on inflammation and enteric permeability in broilers
title_sort effect of day of hatch inoculation with enterobacteriaceae on inflammation and enteric permeability in broilers
publisher The Ohio State University / OhioLINK
publishDate 2021
url http://rave.ohiolink.edu/etdc/view?acc_num=osu1618943283197541
work_keys_str_mv AT chasserkaylinm effectofdayofhatchinoculationwithenterobacteriaceaeoninflammationandentericpermeabilityinbroilers
_version_ 1719490007629037568
spelling ndltd-OhioLink-oai-etd.ohiolink.edu-osu16189432831975412021-10-16T05:25:16Z Effect of day of hatch inoculation with Enterobacteriaceae on inflammation and enteric permeability in broilers Chasser, Kaylin M. Animal Sciences Enterobacteriaceae inflammation alpha-1-glycoprotein yolk sac retention fluorescein isothiocyanate dextran broiler Inappropriate microbial colonization can induce gastrointestinal (GI) inflammation, which may predispose poultry to opportunistic infections and reduce growth performance. Eight independent experiments were completed to test ability of select Enterobacteriaceae isolates to induce GI inflammation. Experiments 3.1 and 3.2 included a non-inoculated control (NC), and a low (L), medium (M), or high (H) day of hatch (DOH) oral inoculation level. In experiment 3.1, birds in L1, M1, and H1 received 10<sup>2</sup>-10<sup>4</sup> CFU of a mixed dose of two species of <i>Citrobacter</i> and <i>Salmonella</i> Enteritidis E2 (SE). In experiment 3.2, birds in L2, M2, and H2 received 10<sup>3</sup>-10<sup>5</sup> CFU of <i>E. coli</i> LG (LG) and included NC. In both experiment 3.1 and 3.2, body weight was recorded on d0, 7, and 14, with blood collected for chicken serum alpha-1-acid glycoprotein (A1GP) measurements on d14. Neither experiment resulted in differences in body weight gain (BWG), however, A1GP was increased (p<0.05) on d14 when DOH inoculation dose 10<sup>3</sup> CFU/chick was used compared to NC. This observed increase in A1GP resulted in selection of 10<sup>3</sup> CFU/chick for DOH inoculation for remaining experiments. Experiment 3.3 consisted of NC, <i>E. coli</i> Huff (Huff), and SE with 10<sup>3</sup> CFU/chick inoculation dose. On d0, 7 and 15, BW was measured, with blood collected on d15 for A1GP. Both d15 A1GP and BWG from d7-15 were reduced in inoculated chicks, Huff and SE, in experiment 3.3 (p<0.05). Experiment 3.4 evaluated NC and LG with BW measured on d0, 2, 7 and 14. Yolk sacs were evaluated for retention and bacterial enumeration, and blood for serum A1GP were collected on d2 and 14. Experiment 4.1 included a non-inoculated control NC, LG, Huff, SE and <i>Salmonella</i> Typhimurium (ST), and consisted of oral inoculation of LG, Huff, and SE at 10<sup>3</sup> CFU/chick, and ST at 10<sup>4</sup> CFU/chick on DOH. Body weight was measured on d0, d, 8, and 15, with yolk sacs (YS) and blood collected for serum A1GP on d2, 8, and 15. Experiment 4.2 included NC, LG, and SE, with BW measured on d0, 2, 6, and 13, and YS, spleen, and blood for A1GP and fluorescein isothiocyanate dextran (FITC-d) recovery on d2, 6, and 13. Experiment 4.3 included NC, LG, Huff, SE, and ST, with BW measured on d0, 2, 8, 15, 22, and 29, YS from d8 and 15, and A1GP and FITC-d from d2, 8, 15, 22, and 29. Experiment 4.4 included DOH inoculation and in feed <i>C. perfringens</i> (CP) challenge on d15 and 16 to induce necrotic enteritis, with non-inoculated non-challenged control, non-inoculated+ <i>C. perfringens</i>, LG+ <i>C. perfringens</i> (LGCP), and Huff+ <i>C. perfringens</i> (HCP). Experiment 3.4 resulted in no differences in yolk sac parameters or A1GP, while there was an increase in BWG for LG from d0-14 (p<0.05). Increased FITC-d recovery was observed for LG and SE on d13 in experiment 4.2 (p<0.05), and NCP birds on d27 in experiment 4.4 (p<0.05) as compared to non-inoculated controls. When evaluated over time, serum A1GP increased between d2 and d14 by nearly 46% in LG, compared to negligible changes in NC (p=0.111). Notably, there were differences in A1GP, particularly in serum concentrations over time. There were fluctuations in early A1GP patterns based on DOH inoculation, but relatively consistent sharp increases were noted in DOH inoculated birds beyond d14 (p<0.05). Greater percent change in A1GP for all DOH inoculated birds from d2-29 was observed in experiment 4.3 (p<0.05), and for NCP and LG birds from d6-27 in experiment 4.4. These results suggest early Enterobacteriaceae exposure may influence immune response development via microbial population shifts in the GI tract. Mild GI inflammation induced by early Enterobacteriaceae exposure may not drastically impact growth or inflammation parameters, but may induce important changes to microbial and immune development. 2021-10-04 English text The Ohio State University / OhioLINK http://rave.ohiolink.edu/etdc/view?acc_num=osu1618943283197541 http://rave.ohiolink.edu/etdc/view?acc_num=osu1618943283197541 unrestricted This thesis or dissertation is protected by copyright: all rights reserved. It may not be copied or redistributed beyond the terms of applicable copyright laws.

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