Pretreatment and Hydrolysis of Whole-plant Corn (WPC) for the Bioproduction of Ethanol

Bibliographic Details
Main Author:	Mbah, Jonathan Ikechukwu
Language:	English
Published:	The Ohio State University / OhioLINK 2020
Subjects:	Alternative Energy Microbiology
Online Access:	http://rave.ohiolink.edu/etdc/view?acc_num=osu1577814083023458

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spelling	ndltd-OhioLink-oai-etd.ohiolink.edu-osu15778140830234582021-08-03T07:13:36Z Pretreatment and Hydrolysis of Whole-plant Corn (WPC) for the Bioproduction of Ethanol Mbah, Jonathan Ikechukwu Alternative Energy Microbiology Three separate studies were conducted to evaluate the pretreatment and hydrolysis of whole plant corn biomass (WPCB) for bioproduction of ethanol. Determination of biomass particle size that would generate optimal sugar during pretreatment and enzymatic hydrolysis was the aim of first study. This was tested with 0.5, 1.0, 2.0, and 3.0 mm particle sizes using 2 processing methods: simultaneous (1 step; combined pretreatment and hydrolysis of WPCB) and sequential (2 steps; initial starch hydrolysis and subsequent lignocellulosic biomass pretreatment and hydrolysis). High Performance Liquid Chromatography (HPLC) was utilized to analyze sugar concentrations. General Linear Model of Minitab Version 16 was utilized to evaluate differences between means while Tukey test was used for mean separation. Sugar analysis result showed no significant difference in both methods among the particle sizes. Marginal increase existed in total sugars with decreased particle sizes in both methods.In the second study, glycerol (0-5 g/L) and tween 80 (0-5 g/L) were supplemented to WPCB in hot water (HW) and 1% sulfuric acid and pretreated at 121 °C for 1 and 2 h. Confirmation of lignocellulosic derived microbial inhibitors compounds (LDMICs) generated and sugar analysis were done using HPLC. Determination of mean differences was by General Linear Model of Minitab Version 16 while Tuckey Test was used to separate means. The results showed no significant increase in total sugars during glycerol and tween 80 supplementation. Results indicated that tween 80 additions marginally increased total sugars by 2-15% in most pretreatment methods. Results of LDMICs generated were diverse showing that tween 80 inclusion in 2 h HW pretreatment depressed inhibitor, glycerol inclusion in HW for 1 h had no effect in inhibitors and tween 80 inclusion in 1 h HW increased inhibitors. Further work is needed for clarification.Third study utilized 50 % of the hydrolysates from WPCB and adjusted each to 180 g/L of sugar with glucose to test fermentability by S. cerevisiae. Glucose and two separate hydrolysates from 1 h HW pretreated 0-3 g/L glycerol were used in first experiment. The second experiment utilized hydrolysates from 2 h 1 % H2SO4 pretreated with 0-5 g/L glycerol. Hydrolysates from 2 h 1 % H2SO4 pretreated with 0-5 g/L Tween 80 were used in third experiment. Each experiment was conducted in triplicates in shaker incubator at 30 ºC and 150 rpm for 108 h. Parameters measured were optical density (OD), ethanol production, and productivity. Analysis of the ethanol generated was conducted using Gas Chromatography. Data analysis was by General Linear Model of Minitab version 16 (Minitab Inc., State College, PA). Parameters analyzed were optical density, ethanol production and productivity. Separation of means was by pair-wise method using Tukey’s test. Results showed that HW pretreated hydrolysates with glycerol and dilute acid pretreated with Tween 80 hydrolysates were better fermented than 1 % H2SO4 pretreated with glycerol. Conclusively, WPCB should be safely pretreated using HW with glycerol addition and dilute acid with Tween 80 addition while using dilute acid pretreatment with glycerol should be approached with caution. 2020-10-02 English text The Ohio State University / OhioLINK http://rave.ohiolink.edu/etdc/view?acc_num=osu1577814083023458 http://rave.ohiolink.edu/etdc/view?acc_num=osu1577814083023458 restricted--full text unavailable until 2022-05-13 This thesis or dissertation is protected by copyright: all rights reserved. It may not be copied or redistributed beyond the terms of applicable copyright laws.
collection	NDLTD
language	English
sources	NDLTD
topic	Alternative Energy Microbiology
spellingShingle	Alternative Energy Microbiology Mbah, Jonathan Ikechukwu Pretreatment and Hydrolysis of Whole-plant Corn (WPC) for the Bioproduction of Ethanol
author	Mbah, Jonathan Ikechukwu
author_facet	Mbah, Jonathan Ikechukwu
author_sort	Mbah, Jonathan Ikechukwu
title	Pretreatment and Hydrolysis of Whole-plant Corn (WPC) for the Bioproduction of Ethanol
title_short	Pretreatment and Hydrolysis of Whole-plant Corn (WPC) for the Bioproduction of Ethanol
title_full	Pretreatment and Hydrolysis of Whole-plant Corn (WPC) for the Bioproduction of Ethanol
title_fullStr	Pretreatment and Hydrolysis of Whole-plant Corn (WPC) for the Bioproduction of Ethanol
title_full_unstemmed	Pretreatment and Hydrolysis of Whole-plant Corn (WPC) for the Bioproduction of Ethanol
title_sort	pretreatment and hydrolysis of whole-plant corn (wpc) for the bioproduction of ethanol
publisher	The Ohio State University / OhioLINK
publishDate	2020
url	http://rave.ohiolink.edu/etdc/view?acc_num=osu1577814083023458
work_keys_str_mv	AT mbahjonathanikechukwu pretreatmentandhydrolysisofwholeplantcornwpcforthebioproductionofethanol
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Pretreatment and Hydrolysis of Whole-plant Corn (WPC) for the Bioproduction of Ethanol

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