Direct Assessment of Osmotic Pressure within Intervertebral Disc Tissue via a Needle Micro-Osmometer
| Main Author: | |
|---|---|
| Language: | English |
| Published: |
The Ohio State University / OhioLINK
2019
|
| Subjects: | |
| Online Access: | http://rave.ohiolink.edu/etdc/view?acc_num=osu1555412949107298 |
| id |
ndltd-OhioLink-oai-etd.ohiolink.edu-osu1555412949107298 |
|---|---|
| record_format |
oai_dc |
| spelling |
ndltd-OhioLink-oai-etd.ohiolink.edu-osu15554129491072982021-08-03T07:10:19Z Direct Assessment of Osmotic Pressure within Intervertebral Disc Tissue via a Needle Micro-Osmometer Keckler, Jesse Biomedical Engineering Biomechanics Osmotic pressure intervertebral disc IVD fixed charge density FCD diurnal pressure Low back pain affects nearly 84% of people at some point in their life, yet the underlying mechanisms are not completely understood. Intervertebral disc disease is commonly associated with low back pain and features a decrease in proteoglycan content in the intervertebral disc (IVD). This decrease in proteoglycan content is directly tied to a drop in osmotic pressure and ion concentration, as the proteoglycans, specifically aggrecan, attract ions in solution and resist the compression of the disc. Additionally, the change in pressure in the disc due to diurnalactivity has been proven to affect the behavior of disc cells, although the true change in osmotic pressure and ion concentration is still unknown. This study aimed to measure the diurnal change in osmotic pressure and ion concentration in a healthy disc by first verifying the use of a needle micro-osmometer in nucleus pulposus (NP) tissue plugs and then in intact discs. Flux measurements using the micro-osmometer were taken in tissue plugs equilibrated in a range of PEG solutions to determine osmotic pressure and ion concentration. The process was thenrepeated in intact IVDs that were mechanically loaded in a diurnal compression cycle. Osmometer flux measurements in NP tissue had a significant relationship with both osmotic pressure and ion concentration, as did intact motion segments. Variation in motion segment measurements were high, restricting use as a predictive tool. The needle micro-osmometer was proven to measure osmotic pressure and ion concentration in both intact IVDs and tissue plugs, although additional testing is needed to reduce error and enhance repeatability in measurements. 2019-08-26 English text The Ohio State University / OhioLINK http://rave.ohiolink.edu/etdc/view?acc_num=osu1555412949107298 http://rave.ohiolink.edu/etdc/view?acc_num=osu1555412949107298 unrestricted This thesis or dissertation is protected by copyright: all rights reserved. It may not be copied or redistributed beyond the terms of applicable copyright laws. |
| collection |
NDLTD |
| language |
English |
| sources |
NDLTD |
| topic |
Biomedical Engineering Biomechanics Osmotic pressure intervertebral disc IVD fixed charge density FCD diurnal pressure |
| spellingShingle |
Biomedical Engineering Biomechanics Osmotic pressure intervertebral disc IVD fixed charge density FCD diurnal pressure Keckler, Jesse Direct Assessment of Osmotic Pressure within Intervertebral Disc Tissue via a Needle Micro-Osmometer |
| author |
Keckler, Jesse |
| author_facet |
Keckler, Jesse |
| author_sort |
Keckler, Jesse |
| title |
Direct Assessment of Osmotic Pressure within Intervertebral Disc Tissue via a Needle Micro-Osmometer |
| title_short |
Direct Assessment of Osmotic Pressure within Intervertebral Disc Tissue via a Needle Micro-Osmometer |
| title_full |
Direct Assessment of Osmotic Pressure within Intervertebral Disc Tissue via a Needle Micro-Osmometer |
| title_fullStr |
Direct Assessment of Osmotic Pressure within Intervertebral Disc Tissue via a Needle Micro-Osmometer |
| title_full_unstemmed |
Direct Assessment of Osmotic Pressure within Intervertebral Disc Tissue via a Needle Micro-Osmometer |
| title_sort |
direct assessment of osmotic pressure within intervertebral disc tissue via a needle micro-osmometer |
| publisher |
The Ohio State University / OhioLINK |
| publishDate |
2019 |
| url |
http://rave.ohiolink.edu/etdc/view?acc_num=osu1555412949107298 |
| work_keys_str_mv |
AT kecklerjesse directassessmentofosmoticpressurewithinintervertebraldisctissueviaaneedlemicroosmometer |
| _version_ |
1719455312646242304 |