Phylogeography and Genetic Diversity of the Commercially-Collected Caribbean Blue-Legged Hermit Crab: Implications for Conservation
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| Language: | English |
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The Ohio State University / OhioLINK
2018
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| Online Access: | http://rave.ohiolink.edu/etdc/view?acc_num=osu1543499269936318 |
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ndltd-OhioLink-oai-etd.ohiolink.edu-osu1543499269936318 |
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NDLTD |
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English |
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Biology Genetics Conservation Clibanarius tricolor population genetics phylogeography aquarium trade Caribbean ddRAD seq Sanger sequencing conservation marine invertebrate |
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Biology Genetics Conservation Clibanarius tricolor population genetics phylogeography aquarium trade Caribbean ddRAD seq Sanger sequencing conservation marine invertebrate Stark, Tiara Elizabeth Phylogeography and Genetic Diversity of the Commercially-Collected Caribbean Blue-Legged Hermit Crab: Implications for Conservation |
| author |
Stark, Tiara Elizabeth |
| author_facet |
Stark, Tiara Elizabeth |
| author_sort |
Stark, Tiara Elizabeth |
| title |
Phylogeography and Genetic Diversity of the Commercially-Collected Caribbean Blue-Legged Hermit Crab: Implications for Conservation |
| title_short |
Phylogeography and Genetic Diversity of the Commercially-Collected Caribbean Blue-Legged Hermit Crab: Implications for Conservation |
| title_full |
Phylogeography and Genetic Diversity of the Commercially-Collected Caribbean Blue-Legged Hermit Crab: Implications for Conservation |
| title_fullStr |
Phylogeography and Genetic Diversity of the Commercially-Collected Caribbean Blue-Legged Hermit Crab: Implications for Conservation |
| title_full_unstemmed |
Phylogeography and Genetic Diversity of the Commercially-Collected Caribbean Blue-Legged Hermit Crab: Implications for Conservation |
| title_sort |
phylogeography and genetic diversity of the commercially-collected caribbean blue-legged hermit crab: implications for conservation |
| publisher |
The Ohio State University / OhioLINK |
| publishDate |
2018 |
| url |
http://rave.ohiolink.edu/etdc/view?acc_num=osu1543499269936318 |
| work_keys_str_mv |
AT starktiaraelizabeth phylogeographyandgeneticdiversityofthecommerciallycollectedcaribbeanblueleggedhermitcrabimplicationsforconservation |
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1719454916662001664 |
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ndltd-OhioLink-oai-etd.ohiolink.edu-osu15434992699363182021-08-03T07:09:05Z Phylogeography and Genetic Diversity of the Commercially-Collected Caribbean Blue-Legged Hermit Crab: Implications for Conservation Stark, Tiara Elizabeth Biology Genetics Conservation Clibanarius tricolor population genetics phylogeography aquarium trade Caribbean ddRAD seq Sanger sequencing conservation marine invertebrate In marine environments, phylogeographic approaches have the power to counteract the difficulties of studying small, highly mobile organisms that live in a fluid environment. These analyses are particularly useful for studying exploited species, as understanding connectivity and processes governing gene flow between populations is essential to sustainable management of fisheries. Among the most vulnerable of exploited species are marine invertebrates collected for the ornamental aquarium trade, as commercial culture techniques are largely not addressed and vital data on larval biology is inadequate or remain unknown. The Caribbean blue-legged hermit crab (<i>Clibanarius tricolor</i>) is heavily collected for its role as an aquarium cleaner; however, despite its economic and ecological importance, little is known about their population structure or genetic diversity. Here, we investigate the phylogeography and genetic diversity of <i>C. tricolor</i> by comparing structure across four well-defined phytogeographic breaks throughout their range in the Caribbean and west Atlantic: the Florida Straits, Mona Passage, Central America, and central Bahamas breaks. We also explore additional factors (e.g. reproductive strategies, life-history traits, habitat preference, demographic history, biogeographic processes) that may influence whether structure is present in marine organisms within this region. Prior to analyzing structure throughout <i>C. tricolor</i>’s entire range, a preliminary study was done to determine whether high throughput sequencing approaches (i.e. double digest Restriction-site Associated DNA sequencing) were necessary for detecting phylogeographic patterns in <i>C. tricolor</i>. Findings indicated that Sanger sequencing of traditional loci used in decapod studies (mtDNA: cytochrome c oxidase subunit 1, 16S-rDNA; nuDNA: Histone 3) were sufficient for this study. To infer <i>C. tricolor</i> structure and connectivity, analyses investigating genetic diversity (# segregating sites, # haplotypes, nucleotide (p) and haplotype diversity (h)), population structure (TCS haplotype networks, pairwise FST, AMOVA, SAMOVA, Isolation by Distance), and historical demography (neutrality tests, mismatch distribution) were computed. Both mitochondrial and nuclear data were concordant in finding high levels of genetic diversity and connectivity across all populations sampled, failing to reject the null hypothesis of panmixia. Data analysis reveals no evidence for phylogeographic structure across the four hypothesized phylogeographic breaks, supports a demographic expansion ~300,000 ybp, and suggests <i>C. tricolor</i> has a high dispersal potential. Although the nuclear locus was perceived to be more informative than mitochondrial loci, we attributed it to small sample size and low variability. This study revealed the Caribbean blue-legged hermit crab to be a genetically diverse, highly connected, panmictic marine invertebrate. Population structure analyses found no evidence for phylogeographic structure across the four hypothesized phylogeographic breaks, indicating they are not barriers to gene flow for <i>C. tricolor</i>, suggesting high dispersal abilities. Although our data could not identify source and sink populations, our findings suggest <i>C. tricolor </i>is not at risk and is an example of a healthily traded marine invertebrate. Investigations into additional factors that may influence structure suggest that structure is hard to predict. Therefore, studies on poorly studied exploited species would be beneficial, rather than extrapolating from findings from other species as a proxy. This will hopefully provide more foundational data for similar studies in the future. 2018 English text The Ohio State University / OhioLINK http://rave.ohiolink.edu/etdc/view?acc_num=osu1543499269936318 http://rave.ohiolink.edu/etdc/view?acc_num=osu1543499269936318 unrestricted This thesis or dissertation is protected by copyright: all rights reserved. It may not be copied or redistributed beyond the terms of applicable copyright laws. |