Development and Applications of Functionalized Octatetrayne as Novel Carbon Media in Chromatography

Bibliographic Details
Main Author:	Liu, Jiayi
Language:	English
Published:	The Ohio State University / OhioLINK 2018
Subjects:	Analytical Chemistry Open tubular columns liquid chromatography two-dimensional gas chromatography carbon materials
Online Access:	http://rave.ohiolink.edu/etdc/view?acc_num=osu1519322396476316

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spelling	ndltd-OhioLink-oai-etd.ohiolink.edu-osu15193223964763162021-08-03T07:05:31Z Development and Applications of Functionalized Octatetrayne as Novel Carbon Media in Chromatography Liu, Jiayi Analytical Chemistry Open tubular columns liquid chromatography two-dimensional gas chromatography carbon materials Open tubular capillary columns, in which stationary phases are coated onto inner wall, have been continuously developed for chromatographic use. Functionalized octatetrayne was synthesized and covalently attached to the capillary column inner wall. Thermal processing at elevated temperatures ensured the formation of a uniform ultrathin layer carbon stationary phase. This robust coating method extended the novel carbon stationary phase into open tubular liquid chromatography and comprehensive two-dimensional gas chromatography for the first time. Open tubular liquid chromatography (OTLC) has potentially high efficiency, fast analysis time and low solvent consumption. The chromatographic performance of the carbon capillary column strongly depended on the final curing temperature, sample size and mobile phase composition. The optimal curing temperature of the carbon phase was determined to be 400 °C. Good separations using the carbon column were obtained on the basis of reversed-phase interactions. The high surface area and unique selectivity of the carbon phase led to improved retention and efficiency for the separations of polycyclic aromatic hydrocarbons (PAHs) and nucleosides at isocratic conditions. The separation impedance of the carbon OTLC was more than 100-fold of commerical packed or monolithic columns.Comprehensive two-dimensional gas chromatography (GCxGC) is recognized as a powerful tool for complex mixtures due to its high peak capacity. Due to the unique selectivity of the carbon phase, the carbon column was utilized as the second dimension coupled to a nonpolar first dimension commercial column using a reverse-inject differential flow modulator. The carbon GCxGC was optimized using an experimental design method and the chromatographic performance of the carbon GCxGC was evaluated by separating a mixture of hydrocarbons. The carbon column offered improved separation space and comparable separation power to a commercial HP-INNOWax column. The superior stability of the carbon phase also expanded the separation temperature range up to 350 °C with no column bleeding and maintained selectivity. The carbon GCxGC also provided feasibility and potential for complex separations such as fuel oil samples. Month-to-month and column-to-column reproducibility tests were performed on both the carbon OTLC and GCxGC systems. The low relative standard deviations (<8%) for the retention times of tested species confirmed high durability of the carbon stationary phase for both LC and GCxGC, and excellent reproducibility of the coating method. 2018-09-11 English text The Ohio State University / OhioLINK http://rave.ohiolink.edu/etdc/view?acc_num=osu1519322396476316 http://rave.ohiolink.edu/etdc/view?acc_num=osu1519322396476316 unrestricted This thesis or dissertation is protected by copyright: all rights reserved. It may not be copied or redistributed beyond the terms of applicable copyright laws.
collection	NDLTD
language	English
sources	NDLTD
topic	Analytical Chemistry Open tubular columns liquid chromatography two-dimensional gas chromatography carbon materials
spellingShingle	Analytical Chemistry Open tubular columns liquid chromatography two-dimensional gas chromatography carbon materials Liu, Jiayi Development and Applications of Functionalized Octatetrayne as Novel Carbon Media in Chromatography
author	Liu, Jiayi
author_facet	Liu, Jiayi
author_sort	Liu, Jiayi
title	Development and Applications of Functionalized Octatetrayne as Novel Carbon Media in Chromatography
title_short	Development and Applications of Functionalized Octatetrayne as Novel Carbon Media in Chromatography
title_full	Development and Applications of Functionalized Octatetrayne as Novel Carbon Media in Chromatography
title_fullStr	Development and Applications of Functionalized Octatetrayne as Novel Carbon Media in Chromatography
title_full_unstemmed	Development and Applications of Functionalized Octatetrayne as Novel Carbon Media in Chromatography
title_sort	development and applications of functionalized octatetrayne as novel carbon media in chromatography
publisher	The Ohio State University / OhioLINK
publishDate	2018
url	http://rave.ohiolink.edu/etdc/view?acc_num=osu1519322396476316
work_keys_str_mv	AT liujiayi developmentandapplicationsoffunctionalizedoctatetrayneasnovelcarbonmediainchromatography
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Development and Applications of Functionalized Octatetrayne as Novel Carbon Media in Chromatography

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