Understanding the role of the FACT complex during development in C. elegans
| Main Author: | |
|---|---|
| Language: | English |
| Published: |
The Ohio State University / OhioLINK
2017
|
| Subjects: | |
| Online Access: | http://rave.ohiolink.edu/etdc/view?acc_num=osu1512092327321986 |
| id |
ndltd-OhioLink-oai-etd.ohiolink.edu-osu1512092327321986 |
|---|---|
| record_format |
oai_dc |
| spelling |
ndltd-OhioLink-oai-etd.ohiolink.edu-osu15120923273219862021-08-03T07:04:56Z Understanding the role of the FACT complex during development in C. elegans Suggs, Brittany Z. Biology Genetics Developmental Biology Molecular Biology Embryonic development FACT complex pharyngeal development Maternal to zygotic transition <p>The maternal to zygotic transition is a developmental phase shared by all multicellular organisms. It is during this time that control of the developing embryo is transferred from maternally provided mRNAs and proteins to the zygotic genome. In order for the zygotic genome to be transcribed, transcription factors must be able to access the promoters of their target genes. Prior to this point, the chromatin has been held in a transcriptionally silent state which must be alleviated before transcription can occur. There are several mechanisms by which chromatin can be rearranged into a more transcriptionally competent state. In this work, I focus on the nucleosome reorganizing FACT complex.</p> <p>FACT is a heterodimeric complex composed of SSRP1 and SPT16 and is known to be involved in various functions such as transcription, DNA replication, and DNA repair. Both SSRP1 and SPT16 have been found to be essential for embryonic development, and in their absence, lethality occurs early. While much is known regarding the functions of the FACT complex at the level of the cell, the requirement for FACT during embryogenesis has been a significant impediment to understanding the role of FACT in development on an organismal scale.</p> <p>In this study, I have evaluated the developmental consequence of FACT depletion during the embryonic development of the nematode <i>Caenorhabditis elegans</i> using RNAi and genetic mutants. I provide evidence for a requirement for FACT in the formation of the anterior pharynx and in the maintenance of cell cycle length and progression. Additionally, I investigate the relationship between FACT and APX-1, a well described component of the pharyngeal development pathway, and, from those data, propose two possible models for how FACT may be involved in pharyngeal development. Together, these data begin to show how the cellular functions of FACT impacts the development of the organism as a whole.</p> 2017 English text The Ohio State University / OhioLINK http://rave.ohiolink.edu/etdc/view?acc_num=osu1512092327321986 http://rave.ohiolink.edu/etdc/view?acc_num=osu1512092327321986 unrestricted This thesis or dissertation is protected by copyright: all rights reserved. It may not be copied or redistributed beyond the terms of applicable copyright laws. |
| collection |
NDLTD |
| language |
English |
| sources |
NDLTD |
| topic |
Biology Genetics Developmental Biology Molecular Biology Embryonic development FACT complex pharyngeal development Maternal to zygotic transition |
| spellingShingle |
Biology Genetics Developmental Biology Molecular Biology Embryonic development FACT complex pharyngeal development Maternal to zygotic transition Suggs, Brittany Z. Understanding the role of the FACT complex during development in C. elegans |
| author |
Suggs, Brittany Z. |
| author_facet |
Suggs, Brittany Z. |
| author_sort |
Suggs, Brittany Z. |
| title |
Understanding the role of the FACT complex during development in C. elegans |
| title_short |
Understanding the role of the FACT complex during development in C. elegans |
| title_full |
Understanding the role of the FACT complex during development in C. elegans |
| title_fullStr |
Understanding the role of the FACT complex during development in C. elegans |
| title_full_unstemmed |
Understanding the role of the FACT complex during development in C. elegans |
| title_sort |
understanding the role of the fact complex during development in c. elegans |
| publisher |
The Ohio State University / OhioLINK |
| publishDate |
2017 |
| url |
http://rave.ohiolink.edu/etdc/view?acc_num=osu1512092327321986 |
| work_keys_str_mv |
AT suggsbrittanyz understandingtheroleofthefactcomplexduringdevelopmentincelegans |
| _version_ |
1719453165276889088 |