Commercial Soils as a Potential Vehicle for Antibiotic Resistance Transmission
| Main Author: | |
|---|---|
| Language: | English |
| Published: |
The Ohio State University / OhioLINK
2017
|
| Subjects: | |
| Online Access: | http://rave.ohiolink.edu/etdc/view?acc_num=osu1503298572132004 |
| id |
ndltd-OhioLink-oai-etd.ohiolink.edu-osu1503298572132004 |
|---|---|
| record_format |
oai_dc |
| spelling |
ndltd-OhioLink-oai-etd.ohiolink.edu-osu15032985721320042021-08-03T07:03:57Z Commercial Soils as a Potential Vehicle for Antibiotic Resistance Transmission Bellinger, Christina G. Food Science Microbiology antibiotic resistance soils potting soils metagenomics soil antibiotic resistance There is growing concern as to the continued ability of antibiotics in clinical settings to be effective due to increases in antibiotic resistance in pathogens. This increase is a major threat to human health, with approximately 23,000 annually killed by untreatable bacteria in the United States alone. The environment is a potential reservoir for antibiotic resistance genes and soil is high in bacterial diversity. This study aims to analyze augmented soils, especially a pilot study on their resistome, to assess the potential risk of commercial soil in disseminating antibiotic resistant bacteria to humans and the environment. A study of fifteen commercial soils was conducted using culture-dependent and metagenomic methods, with six noncommercial garden and general environmental soils used for comparison. Serially diluted soil samples were plated on plate count agar (PCA) and individual colonies were picked and transferred to antibiotic-supplemented agar using the following types and concentrations: ampicillin (16g/L), tetracycline (100g/L), erythromycin (100g/L), and lincomycin (16mg/L). DNA was extracted from soil samples, pooled and sequenced by high throughput sequencing. Primers were designed for three resistance genes in relative high abundance, aac(3)-Id, catB6, and MacB. PCR amplification to detect these genes in individual samples was conducted. All samples studied lacked response to at least one antibiotic using culturing methods, on average 66% to ampicillin, 77% to lincomycin, 15% to erythromycin, and 9% to tetracycline. A total of 59 total phyla were represented in the pooled soil sample, representing 2118 different genera, with the vast majority of bacteria present belonging to the phylum Proteobacteria. aminoglycoside resistance genes presented at elevated levels. Aac(3)-Id was detected in five commercial and one noncommercial soil. catB6 was detected in one soil. The ratio of antibiotic resistance gene hits to total 16S rRNA was 0.023, consistent with previous studies of residential soil and much lower than animal manures, which can range from 0.75-3ARG/16S rRNA. In summary, pilot results suggest commercial soils may not represent a major pool of antibiotic resistance genes but more analysis is needed to fully understand their place in the antibiotic resistance ecology. 2017 English text The Ohio State University / OhioLINK http://rave.ohiolink.edu/etdc/view?acc_num=osu1503298572132004 http://rave.ohiolink.edu/etdc/view?acc_num=osu1503298572132004 unrestricted This thesis or dissertation is protected by copyright: all rights reserved. It may not be copied or redistributed beyond the terms of applicable copyright laws. |
| collection |
NDLTD |
| language |
English |
| sources |
NDLTD |
| topic |
Food Science Microbiology antibiotic resistance soils potting soils metagenomics soil antibiotic resistance |
| spellingShingle |
Food Science Microbiology antibiotic resistance soils potting soils metagenomics soil antibiotic resistance Bellinger, Christina G. Commercial Soils as a Potential Vehicle for Antibiotic Resistance Transmission |
| author |
Bellinger, Christina G. |
| author_facet |
Bellinger, Christina G. |
| author_sort |
Bellinger, Christina G. |
| title |
Commercial Soils as a Potential Vehicle for Antibiotic Resistance Transmission |
| title_short |
Commercial Soils as a Potential Vehicle for Antibiotic Resistance Transmission |
| title_full |
Commercial Soils as a Potential Vehicle for Antibiotic Resistance Transmission |
| title_fullStr |
Commercial Soils as a Potential Vehicle for Antibiotic Resistance Transmission |
| title_full_unstemmed |
Commercial Soils as a Potential Vehicle for Antibiotic Resistance Transmission |
| title_sort |
commercial soils as a potential vehicle for antibiotic resistance transmission |
| publisher |
The Ohio State University / OhioLINK |
| publishDate |
2017 |
| url |
http://rave.ohiolink.edu/etdc/view?acc_num=osu1503298572132004 |
| work_keys_str_mv |
AT bellingerchristinag commercialsoilsasapotentialvehicleforantibioticresistancetransmission |
| _version_ |
1719452870967820288 |