Mapping the minimal DNA element required for Loz1 mediated gene repression in the fission yeast Schizosaccharomyces pombe

Mapping the minimal DNA element required for Loz1 mediated gene repression in the fission yeast Schizosaccharomyces pombe

Bibliographic Details
Main Author: Cardona, Carlos A.
Language:English
Published: The Ohio State University / OhioLINK 2017
Subjects:
Nutrition
zinc
loz1
pombe
spombe
Online Access:http://rave.ohiolink.edu/etdc/view?acc_num=osu1493924732969299
id ndltd-OhioLink-oai-etd.ohiolink.edu-osu1493924732969299
record_format oai_dc
spelling ndltd-OhioLink-oai-etd.ohiolink.edu-osu14939247329692992021-08-03T07:02:19Z Mapping the minimal DNA element required for Loz1 mediated gene repression in the fission yeast Schizosaccharomyces pombe Cardona, Carlos A. Nutrition zinc loz1 pombe spombe Zinc is an essential trace element required for the function of many enzymes and transcription factors. However, zinc in excess is toxic and intracellular zinc levels must be regulated to maintain homeostasis. In the fission yeast model system Schizosaccharomyces pombe, Loz1 is a transcription factor capable of repressing gene expression under high zinc concentrations. Many Loz1 target genes contain a GNNGATC DNA sequence in their promoter, suggesting that Loz1 directly binds to this Loz1 Response Element (LRE) to repress genetic repression. Using a minimal reporter system, the role of the GNNGATC element in Loz1-dependent regulation has been assessed to determine if this sequence is necessary and sufficient to confer genetic repression under high zinc conditions in-vivo. Additionally, Loz1 target genes pof1 and vel1 were evaluated by lacZ reporter constructs to test whether they were regulated by zinc and Loz1. Both target genes were regulated by zinc in a manner dependent upon Loz1. Mutations in the LRE of the vel1 promoter led to a loss of repression under high zinc concentrations in a wild-type cell indicating that this element is necessary for Loz1 function. In the minimal reporter system, a single LRE element was not sufficient to confer Loz1-mediated repression in the minimal reporter system. However, addition of four LRE were required to repress gene expression in a wild-type cell. This regulation was no longer observed in strains lacking the Loz1 protein. This study shows that Loz1 directly binds to this GNNGATC motif to repress gene expression under high zinc concentrations. Moreover, this study suggests that multiple LRE are required for Loz1 to exert its full effects on zinc-dependent gene expression. 2017-10-23 English text The Ohio State University / OhioLINK http://rave.ohiolink.edu/etdc/view?acc_num=osu1493924732969299 http://rave.ohiolink.edu/etdc/view?acc_num=osu1493924732969299 unrestricted This thesis or dissertation is protected by copyright: all rights reserved. It may not be copied or redistributed beyond the terms of applicable copyright laws.
collection NDLTD
language English
sources NDLTD
topic Nutrition
zinc
loz1
pombe
spombe
spellingShingle Nutrition
zinc
loz1
pombe
spombe
Cardona, Carlos A.
Mapping the minimal DNA element required for Loz1 mediated gene repression in the fission yeast Schizosaccharomyces pombe
author Cardona, Carlos A.
author_facet Cardona, Carlos A.
author_sort Cardona, Carlos A.
title Mapping the minimal DNA element required for Loz1 mediated gene repression in the fission yeast Schizosaccharomyces pombe
title_short Mapping the minimal DNA element required for Loz1 mediated gene repression in the fission yeast Schizosaccharomyces pombe
title_full Mapping the minimal DNA element required for Loz1 mediated gene repression in the fission yeast Schizosaccharomyces pombe
title_fullStr Mapping the minimal DNA element required for Loz1 mediated gene repression in the fission yeast Schizosaccharomyces pombe
title_full_unstemmed Mapping the minimal DNA element required for Loz1 mediated gene repression in the fission yeast Schizosaccharomyces pombe
title_sort mapping the minimal dna element required for loz1 mediated gene repression in the fission yeast schizosaccharomyces pombe
publisher The Ohio State University / OhioLINK
publishDate 2017
url http://rave.ohiolink.edu/etdc/view?acc_num=osu1493924732969299
work_keys_str_mv AT cardonacarlosa mappingtheminimaldnaelementrequiredforloz1mediatedgenerepressioninthefissionyeastschizosaccharomycespombe
_version_ 1719452454827851776

Similar Items