A New Role for Vitamin D Binding Protein in Bipolar Disorder
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The Ohio State University / OhioLINK
2017
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| Online Access: | http://rave.ohiolink.edu/etdc/view?acc_num=osu1492642404941773 |
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ndltd-OhioLink-oai-etd.ohiolink.edu-osu14926424049417732021-08-03T07:01:56Z A New Role for Vitamin D Binding Protein in Bipolar Disorder Petrov, Brawnie Rebecca Nutrition vitamin D binding protein bipolar disorder major depressive disorder inflammation DBP-MAF macrophage activating factor inflammation western blot glycosylation actin immunoprecipitation bait Genetic, dietary and inflammatory factors contribute to the etiology of major mood disorders (MMD), thus impeding the identification of specific biomarkers to assist in diagnosis and treatment. We tested the association of vitamin D and inflammatory markers in 36 adolescents with bipolar disorder (BD) and major depressive disorder (MDD) forms of MMD and without MMD (non-mood control). We also assessed the overall level of inflammation using a cell-based reporter assay for nuclear factor-kappa B activation and measuring antibodies to oxidized LDL. We found that these factors were similar between non-mood and MMD youth. To identify potential biomarkers, we developed a screening immunoprecipitation-sequencing approach based on inflammatory brain glia maturation factor beta (GMFß). We discovered that a homologue of GMFß in human plasma is low molecular weight vitamin D binding protein (DBP-L). DBP-L was 8 times more abundant in plasma of BD compared to control participants. DBP-L is a product of enzymatic cleavage, transforming DBP into a macrophage-activating factor. DBP levels in participants’ plasma were validated using western blot because mono- and polyclonal ELISA did not recognize DBP-L. We found significantly increased levels of total DBP and DBP-L in BD compared to control participants. The DBP responds early to cellular damage by scavenging structural proteins and activating inflammatory cells. Our data suggest DBP and DBP-L as marker candidates of BD warranting their validation in a larger cohort of adolescent and adult MMD patients. 2017-08-03 English text The Ohio State University / OhioLINK http://rave.ohiolink.edu/etdc/view?acc_num=osu1492642404941773 http://rave.ohiolink.edu/etdc/view?acc_num=osu1492642404941773 unrestricted This thesis or dissertation is protected by copyright: some rights reserved. It is licensed for use under a Creative Commons license. Specific terms and permissions are available from this document's record in the OhioLINK ETD Center. |
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NDLTD |
| language |
English |
| sources |
NDLTD |
| topic |
Nutrition vitamin D binding protein bipolar disorder major depressive disorder inflammation DBP-MAF macrophage activating factor inflammation western blot glycosylation actin immunoprecipitation bait |
| spellingShingle |
Nutrition vitamin D binding protein bipolar disorder major depressive disorder inflammation DBP-MAF macrophage activating factor inflammation western blot glycosylation actin immunoprecipitation bait Petrov, Brawnie Rebecca A New Role for Vitamin D Binding Protein in Bipolar Disorder |
| author |
Petrov, Brawnie Rebecca |
| author_facet |
Petrov, Brawnie Rebecca |
| author_sort |
Petrov, Brawnie Rebecca |
| title |
A New Role for Vitamin D Binding Protein in Bipolar Disorder |
| title_short |
A New Role for Vitamin D Binding Protein in Bipolar Disorder |
| title_full |
A New Role for Vitamin D Binding Protein in Bipolar Disorder |
| title_fullStr |
A New Role for Vitamin D Binding Protein in Bipolar Disorder |
| title_full_unstemmed |
A New Role for Vitamin D Binding Protein in Bipolar Disorder |
| title_sort |
new role for vitamin d binding protein in bipolar disorder |
| publisher |
The Ohio State University / OhioLINK |
| publishDate |
2017 |
| url |
http://rave.ohiolink.edu/etdc/view?acc_num=osu1492642404941773 |
| work_keys_str_mv |
AT petrovbrawnierebecca anewroleforvitamindbindingproteininbipolardisorder AT petrovbrawnierebecca newroleforvitamindbindingproteininbipolardisorder |
| _version_ |
1719452026046251008 |