The Natural Acquisition of the Oral Microbiome in Childhood: A Cross-Sectional Analysis
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| Language: | English |
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The Ohio State University / OhioLINK
2016
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| Online Access: | http://rave.ohiolink.edu/etdc/view?acc_num=osu1468773162 |
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ndltd-OhioLink-oai-etd.ohiolink.edu-osu14687731622021-08-03T06:37:35Z The Natural Acquisition of the Oral Microbiome in Childhood: A Cross-Sectional Analysis Gandhi, Roma Dentistry Microbiology This cross-sectional study explored the development of the oral microbiome throughout childhood. Our previous studies of infants up to 1 year of age have shown early presence of exogenous species not commonly found in the oral cavity followed by rapid replacement with a small, shared core set of oral bacterial species. Following this initial colonization, we hypothesize that the complexity of the microbial community will steadily increase with advancing age as the oral cavity develops more intricate environmental niches for bacterial growth, and as children are exposed to new strains of bacteria and novel foods.We sampled 116 children and adolescents ranging from age 1 to 14 years and collected salivary, supragingival and subgingival samples. Bacterial community composition was analyzed at the level of species using rRNA gene amplicon sequencing. This data allowed us to determine commonality among core species and the relationship of age to microbial complexity and community composition. Understanding when the establishment of bacterial communities will occur will help us determine if species are acquired in a specific order and will provide clues as to whether some species require the presence of others to colonize. Taken together, insight will be provided into the reconstruction of the natural acquisition of the human oral microbiome from birth through the establishment of the permanent dentition.Changes in species complexity and the establishment of shared order of the oral microbiota have been examined in relation to age and site-specific samples. More specifically, our analyses suggest that the overall oral microbiome remains fairly stable after the first year of life, with very little influence from age, particular oral niches, and caries status. 2016-10-31 English text The Ohio State University / OhioLINK http://rave.ohiolink.edu/etdc/view?acc_num=osu1468773162 http://rave.ohiolink.edu/etdc/view?acc_num=osu1468773162 unrestricted This thesis or dissertation is protected by copyright: all rights reserved. It may not be copied or redistributed beyond the terms of applicable copyright laws. |
| collection |
NDLTD |
| language |
English |
| sources |
NDLTD |
| topic |
Dentistry Microbiology |
| spellingShingle |
Dentistry Microbiology Gandhi, Roma The Natural Acquisition of the Oral Microbiome in Childhood: A Cross-Sectional Analysis |
| author |
Gandhi, Roma |
| author_facet |
Gandhi, Roma |
| author_sort |
Gandhi, Roma |
| title |
The Natural Acquisition of the Oral Microbiome in Childhood: A Cross-Sectional Analysis |
| title_short |
The Natural Acquisition of the Oral Microbiome in Childhood: A Cross-Sectional Analysis |
| title_full |
The Natural Acquisition of the Oral Microbiome in Childhood: A Cross-Sectional Analysis |
| title_fullStr |
The Natural Acquisition of the Oral Microbiome in Childhood: A Cross-Sectional Analysis |
| title_full_unstemmed |
The Natural Acquisition of the Oral Microbiome in Childhood: A Cross-Sectional Analysis |
| title_sort |
natural acquisition of the oral microbiome in childhood: a cross-sectional analysis |
| publisher |
The Ohio State University / OhioLINK |
| publishDate |
2016 |
| url |
http://rave.ohiolink.edu/etdc/view?acc_num=osu1468773162 |
| work_keys_str_mv |
AT gandhiroma thenaturalacquisitionoftheoralmicrobiomeinchildhoodacrosssectionalanalysis AT gandhiroma naturalacquisitionoftheoralmicrobiomeinchildhoodacrosssectionalanalysis |
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1719440462322860032 |