DNA Origami Breadboard: A Platform for Cell Activation and Cell Membrane Functionalization
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The Ohio State University / OhioLINK
2016
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| Online Access: | http://rave.ohiolink.edu/etdc/view?acc_num=osu1461163132 |
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ndltd-OhioLink-oai-etd.ohiolink.edu-osu14611631322021-08-03T06:35:58Z DNA Origami Breadboard: A Platform for Cell Activation and Cell Membrane Functionalization Mollica, Molly Y. Mechanical Engineering Biomechanics Biomedical Engineering Cellular Biology DNA nanotechnology DNA origami DNA breadboard cell membrane receptor-ligand Structural DNA Nanotechnology (“DNA origami”) techniques have enabled the design and synthesis of complex 3D nanostructures with dynamically controllable features that exploit molecular self-assembly principles. Any component that can be conjugated to an oligonucleotide (oligo) can be attached to a DNA nanostructure at a specific location and quantity with nanometer resolution. This includes some fluorescent dyes, quenchers, peptides, RNA, steroids, vitamins, and, by extension, all molecules capable of biotinylation. A DNA origami “breadboard” with 34 strategically located attachment points can therefore be functionalized with a wide variety of components and used for a multitude of purposes. In this thesis, the design, fabrication, purification, characterization, and application of a 68 x 25 x 6 nanometer honeycomb lattice DNA nanostructure will be presented for use in two distinct functions. In the first, a biotinylated antibody was added to the platform and used to better mimic a cell-to-cell receptor-ligand interaction with tunable antibody quantity, location, and flexibility (i.e. range of motion). This led to determination that ligand flexibility, which can be controlled using DNA origami, influences strength of cell activation. In the second, cholesterol-modified oligonucleotides were added to cells and used to anchor the nanostructures onto the cell surface. The ability to integrate DNA origami nanostructures into a cell membrane can enable a wide variety of applications such as a intracellular force sensing, programmed cell-cell adhesion, or triggered recruiting of biomolecules from solution. 2016-08-30 English text The Ohio State University / OhioLINK http://rave.ohiolink.edu/etdc/view?acc_num=osu1461163132 http://rave.ohiolink.edu/etdc/view?acc_num=osu1461163132 unrestricted This thesis or dissertation is protected by copyright: all rights reserved. It may not be copied or redistributed beyond the terms of applicable copyright laws. |
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NDLTD |
| language |
English |
| sources |
NDLTD |
| topic |
Mechanical Engineering Biomechanics Biomedical Engineering Cellular Biology DNA nanotechnology DNA origami DNA breadboard cell membrane receptor-ligand |
| spellingShingle |
Mechanical Engineering Biomechanics Biomedical Engineering Cellular Biology DNA nanotechnology DNA origami DNA breadboard cell membrane receptor-ligand Mollica, Molly Y. DNA Origami Breadboard: A Platform for Cell Activation and Cell Membrane Functionalization |
| author |
Mollica, Molly Y. |
| author_facet |
Mollica, Molly Y. |
| author_sort |
Mollica, Molly Y. |
| title |
DNA Origami Breadboard: A Platform for Cell Activation and Cell Membrane Functionalization |
| title_short |
DNA Origami Breadboard: A Platform for Cell Activation and Cell Membrane Functionalization |
| title_full |
DNA Origami Breadboard: A Platform for Cell Activation and Cell Membrane Functionalization |
| title_fullStr |
DNA Origami Breadboard: A Platform for Cell Activation and Cell Membrane Functionalization |
| title_full_unstemmed |
DNA Origami Breadboard: A Platform for Cell Activation and Cell Membrane Functionalization |
| title_sort |
dna origami breadboard: a platform for cell activation and cell membrane functionalization |
| publisher |
The Ohio State University / OhioLINK |
| publishDate |
2016 |
| url |
http://rave.ohiolink.edu/etdc/view?acc_num=osu1461163132 |
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AT mollicamollyy dnaorigamibreadboardaplatformforcellactivationandcellmembranefunctionalization |
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1719439731957170176 |