Systemic levels of inflammatory mediators in periodontitis
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The Ohio State University / OhioLINK
2015
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ndltd-OhioLink-oai-etd.ohiolink.edu-osu14369612452021-08-03T06:31:53Z Systemic levels of inflammatory mediators in periodontitis Malamis, Dimitrios Dentistry Medicine Immunology Molecular Biology Biomedical Research Cellular Biology Periodontitis biomarkers systemic cytokines alarmins HMGB-1 S100A12 chemerin BAFF FGF21 ADMA inflammation mediators periodontal disease Background: Periodontitis has been associated with several medical conditions. Forsome of these medical conditions, periodontitis has been hypothesized to share important pathogenetic mechanisms. Recently, advances in biochemistry have led to the identification of novel inflammatory mediators implicated in some of the chronic medical conditions associated with periodontitis. However, there is a little to no evidence on the systemic levels of such biomarkers in periodontitis. Potential identification of these systemic inflammatory mediators in periodontitis would offer additional support to the potential periodontal-systemic disease association.Methods: 33 systemically healthy persons, 16 periodontally healthy individuals and 17chronic periodontitis patients, were recruited as part of an observational study. Peripheral blood samples were collected from each participant by venipuncture. Blood samples were collected twice from each participant, at two different time points 1-2 weeks apart. For 10 out of the 17 periodontitis patients who returned for reevaluation, following periodontal treatment, a third blood sample was collected. Samples were processed to prepare serum, aliquoted and stored at -80 °C. Aliquoted serum samples were analyzed for HMGB-1, S100A12, chemerin, BAFF, FGF-21 and ADMA levels using commercially available ELISA kits following the manufacturer’s instructions and performing the assay in duplicate wells.Results: The results showed that the average serum levels of HMGB-1 and chemerinwere statistically significantly higher in patients with periodontitis compared to healthysubjects. The levels of S100A12, BAFF, FGF-21 and ADMA were found to be elevatedin the patient group compared to the control group but the differences did not reachstatistical significance. Statistical analysis of the subgroup of patients that received nonsurgical periodontal treatment showed that the systemic levels of all inflammatorymediators, except for FGF-21, decreased following scaling and root planing. However the differences were not statistically significant.Conclusion: Chronic periodontitis patients were found to have significantly higher serumlevels of HMGB-1 and chemerin compared to periodontally healthy controls. The levelsof the remaining 4 inflammatory mediators in the patient group were higher but notstatistically significantly greater than the control group. The above findings support theexisting evidence of an association between periodontitis and other systemicdiseases/conditions and should form the impetus for more extensive research towards the identification of systemic biomarkers for periodontitis. 2015-10-13 English text The Ohio State University / OhioLINK http://rave.ohiolink.edu/etdc/view?acc_num=osu1436961245 http://rave.ohiolink.edu/etdc/view?acc_num=osu1436961245 unrestricted This thesis or dissertation is protected by copyright: all rights reserved. It may not be copied or redistributed beyond the terms of applicable copyright laws. |
| collection |
NDLTD |
| language |
English |
| sources |
NDLTD |
| topic |
Dentistry Medicine Immunology Molecular Biology Biomedical Research Cellular Biology Periodontitis biomarkers systemic cytokines alarmins HMGB-1 S100A12 chemerin BAFF FGF21 ADMA inflammation mediators periodontal disease |
| spellingShingle |
Dentistry Medicine Immunology Molecular Biology Biomedical Research Cellular Biology Periodontitis biomarkers systemic cytokines alarmins HMGB-1 S100A12 chemerin BAFF FGF21 ADMA inflammation mediators periodontal disease Malamis, Dimitrios Systemic levels of inflammatory mediators in periodontitis |
| author |
Malamis, Dimitrios |
| author_facet |
Malamis, Dimitrios |
| author_sort |
Malamis, Dimitrios |
| title |
Systemic levels of inflammatory mediators in periodontitis |
| title_short |
Systemic levels of inflammatory mediators in periodontitis |
| title_full |
Systemic levels of inflammatory mediators in periodontitis |
| title_fullStr |
Systemic levels of inflammatory mediators in periodontitis |
| title_full_unstemmed |
Systemic levels of inflammatory mediators in periodontitis |
| title_sort |
systemic levels of inflammatory mediators in periodontitis |
| publisher |
The Ohio State University / OhioLINK |
| publishDate |
2015 |
| url |
http://rave.ohiolink.edu/etdc/view?acc_num=osu1436961245 |
| work_keys_str_mv |
AT malamisdimitrios systemiclevelsofinflammatorymediatorsinperiodontitis |
| _version_ |
1719438531115352064 |