Single Molecule Electron Paramagnetic Resonance and Other Sensing and Imaging Applications with Nitrogen-Vacancy Nanodiamond

Bibliographic Details
Main Author:	Teeling-Smith, Richelle Marie
Language:	English
Published:	The Ohio State University / OhioLINK 2015
Subjects:	Biochemistry Biophysics Condensed Matter Physics Molecular Physics Nanoscience Nanotechnology Optics Physics Scientific Imaging NV diamond Nitrogen-Vacancy Diamond EPR Electron Paramagnetic Resonance DNA DNA dynamics confocal microscopy single molecule spectroscopy single-molecule measurements biophysics biomolecular dynamics ODMR Optically detected magnetic resonance
Online Access:	http://rave.ohiolink.edu/etdc/view?acc_num=osu1424779811

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spelling	ndltd-OhioLink-oai-etd.ohiolink.edu-osu14247798112021-08-03T06:29:18Z Single Molecule Electron Paramagnetic Resonance and Other Sensing and Imaging Applications with Nitrogen-Vacancy Nanodiamond Teeling-Smith, Richelle Marie Biochemistry Biophysics Condensed Matter Physics Molecular Physics Nanoscience Nanotechnology Optics Physics Scientific Imaging NV diamond Nitrogen-Vacancy Diamond EPR Electron Paramagnetic Resonance DNA DNA dynamics confocal microscopy single molecule spectroscopy single-molecule measurements biophysics biomolecular dynamics ODMR Optically detected magnetic resonance Electron paramagnetic resonance (EPR) is an established and powerful tool for studying atomic-scale biomolecular structure and dynamics. Yet it requires a homogeneous sample size of approximately 10^15 spin-labeled biomolecules. In contrast, single molecule measurements provide improved insights into heterogeneous behaviors that can be masked by ensemble measurements and are often essential for illuminating the molecular mechanisms behind the function of a biomolecule. In this dissertation, I report EPR measurements of a single labeled biomolecule demonstrating the merging of these two powerful techniques. We have selectively labeled individual double-stranded DNA molecules with nanodiamonds containing nitrogen-vacancy (NV) centers, and optically detected the paramagnetic resonance of the NV nanodiamond probe. Analysis of the spectrum reveals that the characteristic time scale for reorientation of the labeled molecule relative to the applied magnetic field is slow compared to the spin relaxation time of the nanodiamond probe. This demonstration of EPR spectroscopic determination of the dynamics of an individual labeled biomolecular construct provides the foundation for single molecule magnetic resonance studies of complex biomolecular systems.In addition to this single-molecule EPR study, I report on the use of the NV center in diamond as a probe for in-vivo magnetometry, in-vivo fluorescence imaging and temperature sensing, and a tool for the measure of spin resonances in neighboring systems. The NV center in diamond has proven to be powerful tool for sensing and imaging in various systems, with a broad array of undeveloped or underdeveloped applications. The studies included in this dissertation provide a brief overview of a select few experiments that explore these capabilities. 2015-05-21 English text The Ohio State University / OhioLINK http://rave.ohiolink.edu/etdc/view?acc_num=osu1424779811 http://rave.ohiolink.edu/etdc/view?acc_num=osu1424779811 unrestricted This thesis or dissertation is protected by copyright: all rights reserved. It may not be copied or redistributed beyond the terms of applicable copyright laws.
collection	NDLTD
language	English
sources	NDLTD
topic	Biochemistry Biophysics Condensed Matter Physics Molecular Physics Nanoscience Nanotechnology Optics Physics Scientific Imaging NV diamond Nitrogen-Vacancy Diamond EPR Electron Paramagnetic Resonance DNA DNA dynamics confocal microscopy single molecule spectroscopy single-molecule measurements biophysics biomolecular dynamics ODMR Optically detected magnetic resonance
spellingShingle	Biochemistry Biophysics Condensed Matter Physics Molecular Physics Nanoscience Nanotechnology Optics Physics Scientific Imaging NV diamond Nitrogen-Vacancy Diamond EPR Electron Paramagnetic Resonance DNA DNA dynamics confocal microscopy single molecule spectroscopy single-molecule measurements biophysics biomolecular dynamics ODMR Optically detected magnetic resonance Teeling-Smith, Richelle Marie Single Molecule Electron Paramagnetic Resonance and Other Sensing and Imaging Applications with Nitrogen-Vacancy Nanodiamond
author	Teeling-Smith, Richelle Marie
author_facet	Teeling-Smith, Richelle Marie
author_sort	Teeling-Smith, Richelle Marie
title	Single Molecule Electron Paramagnetic Resonance and Other Sensing and Imaging Applications with Nitrogen-Vacancy Nanodiamond
title_short	Single Molecule Electron Paramagnetic Resonance and Other Sensing and Imaging Applications with Nitrogen-Vacancy Nanodiamond
title_full	Single Molecule Electron Paramagnetic Resonance and Other Sensing and Imaging Applications with Nitrogen-Vacancy Nanodiamond
title_fullStr	Single Molecule Electron Paramagnetic Resonance and Other Sensing and Imaging Applications with Nitrogen-Vacancy Nanodiamond
title_full_unstemmed	Single Molecule Electron Paramagnetic Resonance and Other Sensing and Imaging Applications with Nitrogen-Vacancy Nanodiamond
title_sort	single molecule electron paramagnetic resonance and other sensing and imaging applications with nitrogen-vacancy nanodiamond
publisher	The Ohio State University / OhioLINK
publishDate	2015
url	http://rave.ohiolink.edu/etdc/view?acc_num=osu1424779811
work_keys_str_mv	AT teelingsmithrichellemarie singlemoleculeelectronparamagneticresonanceandothersensingandimagingapplicationswithnitrogenvacancynanodiamond
_version_	1719437717214855168

Single Molecule Electron Paramagnetic Resonance and Other Sensing and Imaging Applications with Nitrogen-Vacancy Nanodiamond

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