Proliferation and adhesion of human Gingival Fibroblasts on various dental polymer materials

Bibliographic Details
Main Author:	Young, Duprane Pedaci
Language:	English
Published:	The Ohio State University / OhioLINK 1999
Subjects:	Biomedical Engineering
Online Access:	http://rave.ohiolink.edu/etdc/view?acc_num=osu1413475378

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spelling	ndltd-OhioLink-oai-etd.ohiolink.edu-osu14134753782021-08-03T06:27:54Z Proliferation and adhesion of human Gingival Fibroblasts on various dental polymer materials Young, Duprane Pedaci Biomedical Engineering Triethylene glycol dimethacrylate (TEGDMA), a common component in dental resins, is cytotoxic and can leach out of cured resin materials. A fluorometric cell proliferation assay, a radiolabeled protein assay, and HPLC were performed to evaluate if the amount of TEGDMA in dental resins effects cell proliferation. The materials used were 50:50 2,2-bis[2(2-hydroxy-3-methacryloxypropoxy)phenol]propane (BisGMA)/TEGDMA, 50:50 2,2-bis[4(2-methacryloxyethoxy)phenol]propane (BisEMA)/TEGDMA, 90:10 BisEMA/TEGDMA, and 100% BisEMA, and (as a control material) tissue culture polystyrene. Polystyrene displayed the most cell proliferation of all materials over 7 days. The 100% BisEMA displayed the most cell proliferation of the experimental materials over 7 days, although the results were not statistically significantly greater than the other experimental materials. The amount of collagen production in the protein assay was similar to the results of the cell proliferation results; polystyrene was the highest and there were no statistical differences among experimental materials. The non-collagen assay results were highly variable and inconclusive. No monomers or additives were detected using reverse phase HPLC.Although the materials without TEGDMA resulted in the greatest cell proliferation, the HPLC results indicate that the difference found in this study is not a result of leaching of residual TEGDMA monomer. These results may indicate that the TEGDMA containing materials have different surface characteristics than the materials without TEGDMA.Surface treatment of a material can affect cell proliferation on a material. Cleaning discs, adding a net charge, or adding attachment proteins can improve cell proliferation by making the surface more conducive to cellular growth. A cell proliferation assay was performed using three surface treatment groups. Group 1 was composed of newly fabricated discs, Group 2 contained discs that were used in previous cell proliferation studies and were cleaned in trypsin, and Group 3 contained discs that were cleaned with supercritical CO2 prior to use in this experiment. The results indicate that previously used discs (Group 2) promote the greatest amount of cell proliferation while newly fabricated discs promoted the least amount of cell proliferation.Cell adhesion to material surfaces is necessary for cell growth and proliferation; however, the type of surface that promotes the best cell adhesion is not clear. Many studies indicate that hydrophilic materials promote greater cell adhesion, but a few indicate that hydrophobic materials promote greater cell adhesion. An adhesion assay was performed on polystyrene (PS), poly(ethyl methacrylate) (PEMA) and poly(trifluoroethyl methacrylate) (FPEMA) to determine which materials promoted the greatest amount of cell adhesion. FPEMA is slightly more hydrophobic than PEMA, and FPEMA may have a net negative charge due to the electronegativity of fluorine. Also, the surface of tissue culture PS is changed by a net positive charge placed on it by the manufacturer. The results indicate that FPEMA promotes the greatest amount of cellular adhesion, and that PS promotes the lowest degree of adhesion, and that a moderate degree of hydrophobicity and/or a small negative surface charge may promote cell adhesion. 1999 English text The Ohio State University / OhioLINK http://rave.ohiolink.edu/etdc/view?acc_num=osu1413475378 http://rave.ohiolink.edu/etdc/view?acc_num=osu1413475378 unrestricted This thesis or dissertation is protected by copyright: all rights reserved. It may not be copied or redistributed beyond the terms of applicable copyright laws.
collection	NDLTD
language	English
sources	NDLTD
topic	Biomedical Engineering
spellingShingle	Biomedical Engineering Young, Duprane Pedaci Proliferation and adhesion of human Gingival Fibroblasts on various dental polymer materials
author	Young, Duprane Pedaci
author_facet	Young, Duprane Pedaci
author_sort	Young, Duprane Pedaci
title	Proliferation and adhesion of human Gingival Fibroblasts on various dental polymer materials
title_short	Proliferation and adhesion of human Gingival Fibroblasts on various dental polymer materials
title_full	Proliferation and adhesion of human Gingival Fibroblasts on various dental polymer materials
title_fullStr	Proliferation and adhesion of human Gingival Fibroblasts on various dental polymer materials
title_full_unstemmed	Proliferation and adhesion of human Gingival Fibroblasts on various dental polymer materials
title_sort	proliferation and adhesion of human gingival fibroblasts on various dental polymer materials
publisher	The Ohio State University / OhioLINK
publishDate	1999
url	http://rave.ohiolink.edu/etdc/view?acc_num=osu1413475378
work_keys_str_mv	AT youngdupranepedaci proliferationandadhesionofhumangingivalfibroblastsonvariousdentalpolymermaterials
_version_	1719437281673084928

Proliferation and adhesion of human Gingival Fibroblasts on various dental polymer materials

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