Nanoscale adhesion, friction and wear of proteins on polystyrene

Nanoscale adhesion, friction and wear of proteins on polystyrene

Bibliographic Details
Main Author: Utter, Jason Richard
Language:English
Published: The Ohio State University / OhioLINK 2012
Subjects:
Mechanical Engineering
proteins
adhesion
friction
wear
nanoscale
afm
polystyrene
Online Access:http://rave.ohiolink.edu/etdc/view?acc_num=osu1348681051
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spelling ndltd-OhioLink-oai-etd.ohiolink.edu-osu13486810512021-08-03T06:06:31Z Nanoscale adhesion, friction and wear of proteins on polystyrene Utter, Jason Richard Mechanical Engineering proteins adhesion friction wear nanoscale afm polystyrene Protein layers are routinely deployed on biomaterials and biological micro/nanoelectromechanical systems (bioMEMS/NEMS) as a functional layer allowing for specific molecular recognition, binding properties or to facilitate biocompatibility. In addition, uncoated biomaterial surfaces will have uncontrolled protein layers adsorbing to the surface within seconds of implantation, so a pre-defined protein layer will improve the host response. Implanted biomaterials also experience micromotion over time which may degrade any surface protein layers. Degradation of these protein layers may lead to system failure or an unwanted immune response. Therefore, it is important to characterize the interfacial properties of proteins on biomaterial surfaces. In this study, the nanoscale adhesion, friction and wear properties of proteins adsorbed to a spin coated polystyrene surface were measured using atomic force microscopy (AFM) in deionized (DI) water and phosphate buffered saline. Adhesion, friction and wear have been measured for bovine serum albumin (BSA), collagen, fibronectin and streptavidin (STA) in DI water and PBS as a function of protein concentration. These proteins were chosen due to their importance and widespread application in the biotechnology field. Adhesion and friction were also measured for BSA and STA at two different temperatures and different pH values to simulate a biological environment. Based on this study, adhesion, friction and wear mechanisms of the different proteins are discussed. 2012-12-17 English text The Ohio State University / OhioLINK http://rave.ohiolink.edu/etdc/view?acc_num=osu1348681051 http://rave.ohiolink.edu/etdc/view?acc_num=osu1348681051 unrestricted This thesis or dissertation is protected by copyright: all rights reserved. It may not be copied or redistributed beyond the terms of applicable copyright laws.
collection NDLTD
language English
sources NDLTD
topic Mechanical Engineering
proteins
adhesion
friction
wear
nanoscale
afm
polystyrene
spellingShingle Mechanical Engineering
proteins
adhesion
friction
wear
nanoscale
afm
polystyrene
Utter, Jason Richard
Nanoscale adhesion, friction and wear of proteins on polystyrene
author Utter, Jason Richard
author_facet Utter, Jason Richard
author_sort Utter, Jason Richard
title Nanoscale adhesion, friction and wear of proteins on polystyrene
title_short Nanoscale adhesion, friction and wear of proteins on polystyrene
title_full Nanoscale adhesion, friction and wear of proteins on polystyrene
title_fullStr Nanoscale adhesion, friction and wear of proteins on polystyrene
title_full_unstemmed Nanoscale adhesion, friction and wear of proteins on polystyrene
title_sort nanoscale adhesion, friction and wear of proteins on polystyrene
publisher The Ohio State University / OhioLINK
publishDate 2012
url http://rave.ohiolink.edu/etdc/view?acc_num=osu1348681051
work_keys_str_mv AT utterjasonrichard nanoscaleadhesionfrictionandwearofproteinsonpolystyrene
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