Crevicular Fluid Content during Wound Healing Comparison between Tooth and Implant
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The Ohio State University / OhioLINK
2011
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ndltd-OhioLink-oai-etd.ohiolink.edu-osu13068657512021-08-03T06:02:58Z Crevicular Fluid Content during Wound Healing Comparison between Tooth and Implant Emecen Huja, Pinar Dentistry Wound healing crevicular fluid cytokines implant tooth <p>Background: Histological comparison of peri-implant and periodontal tissues reveals several similarities at both hard and soft tissue levels. However, various phases of peri-implant wound healing may differ from periodontal counterpart due to absence of periodontal ligament apparatus and related blood supply. The ultimate goal of this study was to develop diagnostic tools to evaluate peri-implant wound healing during osseointegration. The specific aims included: 1) determine the expression of several pro-inflammatory cytokines, growth factors and tissue byproducts within newly forming peri-implant crevicular fluid, 2) determine any possible differences in peri-implant and periodontal crevicular fluid content during early phases of wound healing and, 3) determine whether the release of specific biomarkers is correlated with clinical parameters classically used in evaluating wound healing.</p><p>Methods: Subjects who were seeking treatment for replacement of a missing mandibular posterior tooth were recruited. Inclusion criteria consisted of non-smokers with no known systemic and/or oral diseases that might affect wound healing. Teeth that were adjacent to the implant site and an anatomically similar tooth at cross-arch position were included as positive (T+) and negative tooth (T-) controls, respectively. Implant and T+ sites had surgical intervention while T- site did not. Peri-implant and gingival crevicular fluid (PICF and GCF) samples were obtained at baseline, and at weeks 1, 2, 3, 6, 8 and 12. Plaque and gingival indices and implant stability were recorded at each visit. Multiplex bead based human cytokines/growth factors immunoassay and MMP fluorokine kits were used to study PICF and GCF total amount of IL-1b, IL-1ra, IL-6, IL-7, IL-8, IL-10, IL-12, bFGF, Eotaxin, MCP-1, MIP-1b, TNF-α, VEGF, MMP-8, MMP-9 and TIMPs. Data was analyzed using multiple model regression analysis corrected with Bonferonni adjustment. Statistical significance level was accepted at P=0.016. </p><p>Results: 40 patients (22F/18M and 21-74yo) completed the study.Plaque levels were well controlled throughout the study. Gingival indices showed a statistically significant decrease around I and T+ sites (P<0.01)) during the early wound healing period and continued to decrease only around the I site during late wound healing phase. PICF volume decreased ~3 fold by the end of the study compared to week 1 (P<0.0001). A gradual increase in implant stability was noted from surgery to 12 weeks (P<0.0001). Some pro-inflammatory biomarkers (IL-6, IL-8, MIP-1b, MMP-9, TIMP-1 and TIMP-2) had high total protein amounts at I and T+ sites at fist week. Statistically significant decreases were noted for these biomarkers at I and T+ sites by the end week 3 compared to week 1 (P<0.016). Crevicular fluid levels of IL-8, MIP-1β and TIMP-1 at T+ sites returned to baseline levels by week 12. T- sites did not present any statistically significant changes in biomarker levels throughout the study period.</p><p>Conclusion: Wound healing around dental implants presents variations in clinical and biological parameters compared to natural tooth site. Several biomarkers have the potential to be diagnostic in evaluating peri-implant wound healing.</p><p>[This study is supported by NIDCR grant R21 DE018718].</p> 2011-07-28 English text The Ohio State University / OhioLINK http://rave.ohiolink.edu/etdc/view?acc_num=osu1306865751 http://rave.ohiolink.edu/etdc/view?acc_num=osu1306865751 unrestricted This thesis or dissertation is protected by copyright: all rights reserved. It may not be copied or redistributed beyond the terms of applicable copyright laws. |
| collection |
NDLTD |
| language |
English |
| sources |
NDLTD |
| topic |
Dentistry Wound healing crevicular fluid cytokines implant tooth |
| spellingShingle |
Dentistry Wound healing crevicular fluid cytokines implant tooth Emecen Huja, Pinar Crevicular Fluid Content during Wound Healing Comparison between Tooth and Implant |
| author |
Emecen Huja, Pinar |
| author_facet |
Emecen Huja, Pinar |
| author_sort |
Emecen Huja, Pinar |
| title |
Crevicular Fluid Content during Wound Healing Comparison between Tooth and Implant |
| title_short |
Crevicular Fluid Content during Wound Healing Comparison between Tooth and Implant |
| title_full |
Crevicular Fluid Content during Wound Healing Comparison between Tooth and Implant |
| title_fullStr |
Crevicular Fluid Content during Wound Healing Comparison between Tooth and Implant |
| title_full_unstemmed |
Crevicular Fluid Content during Wound Healing Comparison between Tooth and Implant |
| title_sort |
crevicular fluid content during wound healing comparison between tooth and implant |
| publisher |
The Ohio State University / OhioLINK |
| publishDate |
2011 |
| url |
http://rave.ohiolink.edu/etdc/view?acc_num=osu1306865751 |
| work_keys_str_mv |
AT emecenhujapinar crevicularfluidcontentduringwoundhealingcomparisonbetweentoothandimplant |
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