Functional Analyses of Human Serum Paraoxonase1 (HuPON1) Mutants Using Drop Coating Deposition Raman Difference Spectroscopy

Functional Analyses of Human Serum Paraoxonase1 (HuPON1) Mutants Using Drop Coating Deposition Raman Difference Spectroscopy

Bibliographic Details
Main Author: Ying, Hua
Language:English
Published: The Ohio State University / OhioLINK 2010
Subjects:
Chemistry
PON1
paraoxon
Raman
DCDR
Raman difference spectroscopy
Online Access:http://rave.ohiolink.edu/etdc/view?acc_num=osu1284657860
id ndltd-OhioLink-oai-etd.ohiolink.edu-osu1284657860
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spelling ndltd-OhioLink-oai-etd.ohiolink.edu-osu12846578602021-08-03T06:00:46Z Functional Analyses of Human Serum Paraoxonase1 (HuPON1) Mutants Using Drop Coating Deposition Raman Difference Spectroscopy Ying, Hua Chemistry PON1 paraoxon Raman DCDR Raman difference spectroscopy We present work on the structural implications of specific mutants of Paraoxonase1 (PON1) G2E6, and the turnover rate upon bonding of the enzymes with paraoxon when compared to the wild-type enzyme by using vibrational spectroscopy. A new Raman spectroscopy called Drop Coating Deposition Raman (DCDR) is utilized in our work. The Raman band changes in the paraoxon/H115W system are in good agreement with computational calculations and are strong evidence of the formation of the paraoxon hydrolysis product, p-nitrophenol in the reaction system. The corresponsive turnover rates of G2E6 wild-type and its two mutants, H115W and H115T, are also observed in DCDR spectra. 2010-11-01 English text The Ohio State University / OhioLINK http://rave.ohiolink.edu/etdc/view?acc_num=osu1284657860 http://rave.ohiolink.edu/etdc/view?acc_num=osu1284657860 unrestricted This thesis or dissertation is protected by copyright: all rights reserved. It may not be copied or redistributed beyond the terms of applicable copyright laws.
collection NDLTD
language English
sources NDLTD
topic Chemistry
PON1
paraoxon
Raman
DCDR
Raman difference spectroscopy
spellingShingle Chemistry
PON1
paraoxon
Raman
DCDR
Raman difference spectroscopy
Ying, Hua
Functional Analyses of Human Serum Paraoxonase1 (HuPON1) Mutants Using Drop Coating Deposition Raman Difference Spectroscopy
author Ying, Hua
author_facet Ying, Hua
author_sort Ying, Hua
title Functional Analyses of Human Serum Paraoxonase1 (HuPON1) Mutants Using Drop Coating Deposition Raman Difference Spectroscopy
title_short Functional Analyses of Human Serum Paraoxonase1 (HuPON1) Mutants Using Drop Coating Deposition Raman Difference Spectroscopy
title_full Functional Analyses of Human Serum Paraoxonase1 (HuPON1) Mutants Using Drop Coating Deposition Raman Difference Spectroscopy
title_fullStr Functional Analyses of Human Serum Paraoxonase1 (HuPON1) Mutants Using Drop Coating Deposition Raman Difference Spectroscopy
title_full_unstemmed Functional Analyses of Human Serum Paraoxonase1 (HuPON1) Mutants Using Drop Coating Deposition Raman Difference Spectroscopy
title_sort functional analyses of human serum paraoxonase1 (hupon1) mutants using drop coating deposition raman difference spectroscopy
publisher The Ohio State University / OhioLINK
publishDate 2010
url http://rave.ohiolink.edu/etdc/view?acc_num=osu1284657860
work_keys_str_mv AT yinghua functionalanalysesofhumanserumparaoxonase1hupon1mutantsusingdropcoatingdepositionramandifferencespectroscopy
_version_ 1719429376399900672

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