The Choice of Housekeeping Gene and Relative Efficiency of Paired t-tests in RT-PCR Data Analysis
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| Language: | English |
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The Ohio State University / OhioLINK
2010
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| Online Access: | http://rave.ohiolink.edu/etdc/view?acc_num=osu1281662656 |
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ndltd-OhioLink-oai-etd.ohiolink.edu-osu12816626562021-08-03T06:00:31Z The Choice of Housekeeping Gene and Relative Efficiency of Paired t-tests in RT-PCR Data Analysis Guo, Yi Real time polymerase chain reaction (RT-PCR), a method of quantitatively measuring gene expression, is widely used in biomedical research. It is a highly sensitive techniquewhich can accurately measure the presence of even trace amounts of mRNA in the cell. To produce reliable results, it is essential to use stably expressed housekeeping genes for data normalization. In this study, a RT-PCR dataset that contains 12 cancer related genes from 16 different tumor tissues and 3 housekeeping genes was analyzed. Black raspberry was used as a treatment against cancer and its effects on the expression of these 12 target genes were examined using a paired design. The objective of this study was to examine the effect of normalization on efficiency of the t-test and the choice of housekeeping gene (HKG) on the identification of differentially expressed genes in RT-PCR experiments. Data analysis showed that results differed with choice of HKG since 2 of the 3 HKGs were differentially expressed across treatment groups. We also found that normalization improved the efficiency of the t-test for most of the target genes. 2010-10-22 English text The Ohio State University / OhioLINK http://rave.ohiolink.edu/etdc/view?acc_num=osu1281662656 http://rave.ohiolink.edu/etdc/view?acc_num=osu1281662656 unrestricted This thesis or dissertation is protected by copyright: all rights reserved. It may not be copied or redistributed beyond the terms of applicable copyright laws. |
| collection |
NDLTD |
| language |
English |
| sources |
NDLTD |
| author |
Guo, Yi |
| spellingShingle |
Guo, Yi The Choice of Housekeeping Gene and Relative Efficiency of Paired t-tests in RT-PCR Data Analysis |
| author_facet |
Guo, Yi |
| author_sort |
Guo, Yi |
| title |
The Choice of Housekeeping Gene and Relative Efficiency of Paired t-tests in RT-PCR Data Analysis |
| title_short |
The Choice of Housekeeping Gene and Relative Efficiency of Paired t-tests in RT-PCR Data Analysis |
| title_full |
The Choice of Housekeeping Gene and Relative Efficiency of Paired t-tests in RT-PCR Data Analysis |
| title_fullStr |
The Choice of Housekeeping Gene and Relative Efficiency of Paired t-tests in RT-PCR Data Analysis |
| title_full_unstemmed |
The Choice of Housekeeping Gene and Relative Efficiency of Paired t-tests in RT-PCR Data Analysis |
| title_sort |
choice of housekeeping gene and relative efficiency of paired t-tests in rt-pcr data analysis |
| publisher |
The Ohio State University / OhioLINK |
| publishDate |
2010 |
| url |
http://rave.ohiolink.edu/etdc/view?acc_num=osu1281662656 |
| work_keys_str_mv |
AT guoyi thechoiceofhousekeepinggeneandrelativeefficiencyofpairedttestsinrtpcrdataanalysis AT guoyi choiceofhousekeepinggeneandrelativeefficiencyofpairedttestsinrtpcrdataanalysis |
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1719429287085342720 |