Characterization of two paralogous genes RADICAL-INDUCED CELL DEATH1 and SIMILAR TO RCD ONE1 in Arabidopsis thaliana

Bibliographic Details
Main Author:	Teotia, Sachin
Language:	English
Published:	The Ohio State University / OhioLINK 2009
Subjects:	Molecular Biology RCD1 SRO1 PARP PRS FIL PAN Arabidopsis
Online Access:	http://rave.ohiolink.edu/etdc/view?acc_num=osu1259609832

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spelling	ndltd-OhioLink-oai-etd.ohiolink.edu-osu12596098322021-08-03T05:57:37Z Characterization of two paralogous genes RADICAL-INDUCED CELL DEATH1 and SIMILAR TO RCD ONE1 in Arabidopsis thaliana Teotia, Sachin Molecular Biology RCD1 SRO1 PARP PRS FIL PAN Arabidopsis Poly(ADP-ribose) polymerases (PARPs) are enzymes that modify target proteins by attaching ADP-ribose chains to them. This type of enzyme is found across the eukaryotes and has been implicated in DNA repair, chromatin remodeling, cell cycle control and many other functions. A land plant specific group of PARPs are characterized by a WWE protein-protein interaction domain N-terminal to the PARP catalytic domain common to all family members. The model plant species Arabidopsis thaliana has two genes, RADICAL-INDUCED CELL DEATH1 (RCD1) and SIMILAR TO RCD ONE 1 (SRO1), which encode such proteins and are paralogs. We isolated mutants in both RCD1 and SRO1, rcd1-3 and sro1-1, respectively. rcd1-3 plants, like previously studied alleles, have pleiotropic phenotypic defects, most notably reduced plant height, altered leaf morphology, early flowering, increased numbers of lateral roots and shorter primary roots. sro1-1 plants resemble wild type, but have more lateral roots and are slightly late flowering. In addition to the developmental defects described above, rcd1-3 and sro1-1 mutants also have abiotic stress response defects. Double mutants between the two genes show severe developmental defects; they are extreme dwarfs, have misshapen leaves, and reduced apical dominance. rcd1-3; sro1-1 plants have very slow growth and make fewer cells. In addition the internal patterning of the stems and leaves is abnormal. In particular, the vascular tissues are mispatterned and appear to have been prematurely differentiated. Most interestingly, most of the rcd1-3; sro1-1 individuals die as embryos, demonstrating that the function(s) encoded by these genes are critical for plants. The severity of the double mutant phenotype also illustrates that RCD1 and SRO1 are partially redundant in controlling Arabidopsis development. Plants exposed to low levels of abiotic stress show a syndrome of developmental defects termed the Stress-Induced Morphogenetic Response (SIMR). This syndrome includes reduced rates of cell division, reduced cell elongation and redirection of growth to axillary meristems. Both the accumulation of reactive oxygen species and changes in the phytohormone auxin have been shown to contribute to SIMR. rcd1 mutants accumulate reactive oxygen species and nitric oxide. We hypothesized that the pleiotropic developmental defects seen in these mutants might be due to constitutive stress leading to constitutive SIMR. Consistent with this hypothesis, rcd1-3; sro1-1 mutants show increased expression of stress-induced genes, even when grown under normal conditions. We therefore conclude that RCD1 and SRO1 normally repress stress responses and that in their absence, constitutive stress signalling leads to abnormal development. 2009 English text The Ohio State University / OhioLINK http://rave.ohiolink.edu/etdc/view?acc_num=osu1259609832 http://rave.ohiolink.edu/etdc/view?acc_num=osu1259609832 unrestricted This thesis or dissertation is protected by copyright: all rights reserved. It may not be copied or redistributed beyond the terms of applicable copyright laws.
collection	NDLTD
language	English
sources	NDLTD
topic	Molecular Biology RCD1 SRO1 PARP PRS FIL PAN Arabidopsis
spellingShingle	Molecular Biology RCD1 SRO1 PARP PRS FIL PAN Arabidopsis Teotia, Sachin Characterization of two paralogous genes RADICAL-INDUCED CELL DEATH1 and SIMILAR TO RCD ONE1 in Arabidopsis thaliana
author	Teotia, Sachin
author_facet	Teotia, Sachin
author_sort	Teotia, Sachin
title	Characterization of two paralogous genes RADICAL-INDUCED CELL DEATH1 and SIMILAR TO RCD ONE1 in Arabidopsis thaliana
title_short	Characterization of two paralogous genes RADICAL-INDUCED CELL DEATH1 and SIMILAR TO RCD ONE1 in Arabidopsis thaliana
title_full	Characterization of two paralogous genes RADICAL-INDUCED CELL DEATH1 and SIMILAR TO RCD ONE1 in Arabidopsis thaliana
title_fullStr	Characterization of two paralogous genes RADICAL-INDUCED CELL DEATH1 and SIMILAR TO RCD ONE1 in Arabidopsis thaliana
title_full_unstemmed	Characterization of two paralogous genes RADICAL-INDUCED CELL DEATH1 and SIMILAR TO RCD ONE1 in Arabidopsis thaliana
title_sort	characterization of two paralogous genes radical-induced cell death1 and similar to rcd one1 in arabidopsis thaliana
publisher	The Ohio State University / OhioLINK
publishDate	2009
url	http://rave.ohiolink.edu/etdc/view?acc_num=osu1259609832
work_keys_str_mv	AT teotiasachin characterizationoftwoparalogousgenesradicalinducedcelldeath1andsimilartorcdone1inarabidopsisthaliana
_version_	1719428435879657472

Characterization of two paralogous genes RADICAL-INDUCED CELL DEATH1 and SIMILAR TO RCD ONE1 in Arabidopsis thaliana

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