Effect of Black Raspberry Extracts on Colon Cancer Cell Proliferation
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The Ohio State University / OhioLINK
2009
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| Online Access: | http://rave.ohiolink.edu/etdc/view?acc_num=osu1244025041 |
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ndltd-OhioLink-oai-etd.ohiolink.edu-osu12440250412021-08-03T05:56:21Z Effect of Black Raspberry Extracts on Colon Cancer Cell Proliferation Johnson, Jodee Lee Food Science Nutrition Black Raspberry HT-29 colon cancer cells Chemoprotective Colon Cancer Total Monomeric Anthocyanins Total Phenolics in vitro <p>Diets rich in colorful fruits and vegetables have been found to correlate with lower incidence of certain cancers. In particular, black raspberries have been shown to inhibit multiple stages of oral, esophageal and colon cancer. At The Ohio State University, the mechanisms by which black raspberry compounds are chemoprotective are under exploration. The objective for this study was to evaluate how compositional variability impacts the antiproliferative activity of 75 black raspberry samples using an <i>in vitro</i> colon cancer cell model.</p><p>Polyphenolics from black raspberries were extracted, semi-purified and lyophilized to obtain black raspberry extract powders. Human HT-29 colon cancer cells grown in 96-well plates were treated with freeze-dried black raspberry extracts at concentrations of 0.6 and 1.2 mg extract/mL media. Percent growth inhibition for each concentration of the extracts was determined using sulforhodamine B assay. Chemical composition of the extracts was also monitored by spectrophotometric and chromatographic techniques.</p><p>All samples tested significantly inhibited the growth of HT-29 colon cancer cells in a dose-dependant manner. Inhibition of cancer cell proliferation obtained with different extracts ranged from 33-118%, with values higher than 100% inhibition indicating cytotoxic effects of the extracts. Cultivar, growing location and maturity all affected compositional characteristics of black raspberries, and these changes affected their ability to inhibit cancer cell proliferation. Data suggests that interaction of different berry components, rather than one class of compounds may be responsible for the inhibitory action of black raspberries on HT-29 colon cancer cells. These data may aid basic scientists, horticulturists, as well as the general population in the identification of fruits with the greatest potential to prevent and/or reduce the development of chronic disease.</p> 2009-09-03 English text The Ohio State University / OhioLINK http://rave.ohiolink.edu/etdc/view?acc_num=osu1244025041 http://rave.ohiolink.edu/etdc/view?acc_num=osu1244025041 unrestricted This thesis or dissertation is protected by copyright: all rights reserved. It may not be copied or redistributed beyond the terms of applicable copyright laws. |
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NDLTD |
| language |
English |
| sources |
NDLTD |
| topic |
Food Science Nutrition Black Raspberry HT-29 colon cancer cells Chemoprotective Colon Cancer Total Monomeric Anthocyanins Total Phenolics in vitro |
| spellingShingle |
Food Science Nutrition Black Raspberry HT-29 colon cancer cells Chemoprotective Colon Cancer Total Monomeric Anthocyanins Total Phenolics in vitro Johnson, Jodee Lee Effect of Black Raspberry Extracts on Colon Cancer Cell Proliferation |
| author |
Johnson, Jodee Lee |
| author_facet |
Johnson, Jodee Lee |
| author_sort |
Johnson, Jodee Lee |
| title |
Effect of Black Raspberry Extracts on Colon Cancer Cell Proliferation |
| title_short |
Effect of Black Raspberry Extracts on Colon Cancer Cell Proliferation |
| title_full |
Effect of Black Raspberry Extracts on Colon Cancer Cell Proliferation |
| title_fullStr |
Effect of Black Raspberry Extracts on Colon Cancer Cell Proliferation |
| title_full_unstemmed |
Effect of Black Raspberry Extracts on Colon Cancer Cell Proliferation |
| title_sort |
effect of black raspberry extracts on colon cancer cell proliferation |
| publisher |
The Ohio State University / OhioLINK |
| publishDate |
2009 |
| url |
http://rave.ohiolink.edu/etdc/view?acc_num=osu1244025041 |
| work_keys_str_mv |
AT johnsonjodeelee effectofblackraspberryextractsoncoloncancercellproliferation |
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1719428116717240320 |