Topical Application of a Bioadhesive Black Raspberry Gel Modulates Gene Expression and Reduces Cyclooxygenase 2 Protein in Human Premalignant Oral Lesions

Topical Application of a Bioadhesive Black Raspberry Gel Modulates Gene Expression and Reduces Cyclooxygenase 2 Protein in Human Premalignant Oral Lesions

Bibliographic Details
Main Author: Zwick, Dr. Jared C.
Language:English
Published: The Ohio State University / OhioLINK 2009
Online Access:http://rave.ohiolink.edu/etdc/view?acc_num=osu1232653286
id ndltd-OhioLink-oai-etd.ohiolink.edu-osu1232653286
record_format oai_dc
spelling ndltd-OhioLink-oai-etd.ohiolink.edu-osu12326532862021-08-03T05:55:05Z Topical Application of a Bioadhesive Black Raspberry Gel Modulates Gene Expression and Reduces Cyclooxygenase 2 Protein in Human Premalignant Oral Lesions Zwick, Dr. Jared C. Reduced expression of pro-apoptotic and terminal differentiation genes in conjunction with increased levels of the proinflammatory and angiogenesis-inducing enzymes cyclooxygenase 2 (COX-2) and inducible nitric oxide synthase (iNOS) correlate with malignant transformation of oral epithelial dysplasia (OED). Accordingly, this study investigated the effects a 10% (w/w) freeze dried black raspberry (FBR) gel on oral OED histopathology, gene expression profiles, intraepithelial COX-2 and iNOS proteins and microvascular densities (MVD). Our laboratories have demonstrated that FBR possess antioxidant properties and also induce keratinocyte apoptosis and terminal differentiation. Oral OED tissues were hemisected to provide samples for pretreatment diagnoses and establish baseline biochemical and molecular parameters. Treatment of the remaining lesional tissue (0.5 grams gel applied four times daily for six weeks) began one week after the initial biopsy. RNA was isolated from snap frozen OED lesions for microarray analyses, followed by QRT-PCR validation. Additional, epithelial gene specific QRT-PCR analyses facilitated assessment of target tissue treatment effects. Surface epithelial COX-2 and iNOS protein levels and MVD were determined by image analysis quantified immunohistochemistry. Topical berry gel application uniformly suppressed genes associated with RNA processing, growth factor recycling and inhibition of apoptosis. While the majority of participants showed posttreatment decreases in epithelial iNOS and COX-2 proteins, only COX-2 reductions were statistically significant. These data demonstrate that berry gel application modulated oral OED gene expression profiles, ultimately reducing epithelial COX-2 protein. In a patient subset, berry gel application also reduced vascular densities in the superficial connective tissues and induced genes associated with keratinocyte terminal differentiation. 2009-02-18 English text The Ohio State University / OhioLINK http://rave.ohiolink.edu/etdc/view?acc_num=osu1232653286 http://rave.ohiolink.edu/etdc/view?acc_num=osu1232653286 unrestricted This thesis or dissertation is protected by copyright: all rights reserved. It may not be copied or redistributed beyond the terms of applicable copyright laws.
collection NDLTD
language English
sources NDLTD
author Zwick, Dr. Jared C.
spellingShingle Zwick, Dr. Jared C.
Topical Application of a Bioadhesive Black Raspberry Gel Modulates Gene Expression and Reduces Cyclooxygenase 2 Protein in Human Premalignant Oral Lesions
author_facet Zwick, Dr. Jared C.
author_sort Zwick, Dr. Jared C.
title Topical Application of a Bioadhesive Black Raspberry Gel Modulates Gene Expression and Reduces Cyclooxygenase 2 Protein in Human Premalignant Oral Lesions
title_short Topical Application of a Bioadhesive Black Raspberry Gel Modulates Gene Expression and Reduces Cyclooxygenase 2 Protein in Human Premalignant Oral Lesions
title_full Topical Application of a Bioadhesive Black Raspberry Gel Modulates Gene Expression and Reduces Cyclooxygenase 2 Protein in Human Premalignant Oral Lesions
title_fullStr Topical Application of a Bioadhesive Black Raspberry Gel Modulates Gene Expression and Reduces Cyclooxygenase 2 Protein in Human Premalignant Oral Lesions
title_full_unstemmed Topical Application of a Bioadhesive Black Raspberry Gel Modulates Gene Expression and Reduces Cyclooxygenase 2 Protein in Human Premalignant Oral Lesions
title_sort topical application of a bioadhesive black raspberry gel modulates gene expression and reduces cyclooxygenase 2 protein in human premalignant oral lesions
publisher The Ohio State University / OhioLINK
publishDate 2009
url http://rave.ohiolink.edu/etdc/view?acc_num=osu1232653286
work_keys_str_mv AT zwickdrjaredc topicalapplicationofabioadhesiveblackraspberrygelmodulatesgeneexpressionandreducescyclooxygenase2proteininhumanpremalignantorallesions
_version_ 1719427772871344128

Similar Items