Fundamental Studies With Functionalized Low Temperature Glassy Carbon In Liquid Chromatography, Solid-Liquid Extraction, And Capillary Electrophoresis
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The Ohio State University / OhioLINK
2008
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| Online Access: | http://rave.ohiolink.edu/etdc/view?acc_num=osu1218477883 |
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ndltd-OhioLink-oai-etd.ohiolink.edu-osu12184778832021-08-03T05:54:20Z Fundamental Studies With Functionalized Low Temperature Glassy Carbon In Liquid Chromatography, Solid-Liquid Extraction, And Capillary Electrophoresis Shearer, Justin W. Analytical Chemistry low temperature glassy carbon liquid chromatography extraction microfabrication electrophoresis fluorinated stationary phase The examination of functionalized low temperature glassy carbon (LTGC) in three areas of separtation science follows. The solvation parameter model by Abraham determined important interactions in liquid chromatographic retention using two fluorine-containing LTGC stationary phases. The solvation parameter model for 200 °C F-LTGC showed similar trends in the importance of interactions as previously observed for octadecyl-polysiloxane stationary phases, while 400 °C F-LTGC shows similar interactions as previously found with porous glassy carbon in that π-π interactions with the carbon surface contribute more to retention. Comparison of solid-phase extraction using resorcinol-formaldehyde carbon cryogels and F-LTGC coated cryogels as adsorbents demonstrated that differences in extraction ability vary with processing temperature. Bare cryogels and F-LTGC coatings thermally processed from 200-600 °C exhibited different extraction performance, while F-LTGC coatings processed at 800 °C and bare cryogels displayed similar extraction performance. Molecules that have significant polarizability and planarity displayed higher adsorption capacities with F-LTGC coated cryogels than similar molecules and shows that solute characteristics, such as dispersive interactions and polarity, contributed favorably to extraction. Soft lithography, replica molding, successfully produced micrometer and nanometer features. Pressure-assisted electrophoresis using silicon-containing LTGC (Si-LTGC) and F-LTGC microchips resulted in observation of differences in performance. F-LTGC microchips produced electropherograms with longer retention times, smaller peak areas, and larger peak widths than Si-LTGC microchips. The electrophoretic performance of fused silica, F-LTGC, and Si-LTGC (both processed to 200 °C was examined. A comparison of electrophoretic peak areas showed that both LTGC surfaces resulted in different performance than the fused silica surface. Both LTGCs produced shorter analysis times with smaller peak areas than on fused silica, which is attributed to analyte sorption on the LTGC surface. 2008-09-11 English text The Ohio State University / OhioLINK http://rave.ohiolink.edu/etdc/view?acc_num=osu1218477883 http://rave.ohiolink.edu/etdc/view?acc_num=osu1218477883 unrestricted This thesis or dissertation is protected by copyright: all rights reserved. It may not be copied or redistributed beyond the terms of applicable copyright laws. |
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NDLTD |
| language |
English |
| sources |
NDLTD |
| topic |
Analytical Chemistry low temperature glassy carbon liquid chromatography extraction microfabrication electrophoresis fluorinated stationary phase |
| spellingShingle |
Analytical Chemistry low temperature glassy carbon liquid chromatography extraction microfabrication electrophoresis fluorinated stationary phase Shearer, Justin W. Fundamental Studies With Functionalized Low Temperature Glassy Carbon In Liquid Chromatography, Solid-Liquid Extraction, And Capillary Electrophoresis |
| author |
Shearer, Justin W. |
| author_facet |
Shearer, Justin W. |
| author_sort |
Shearer, Justin W. |
| title |
Fundamental Studies With Functionalized Low Temperature Glassy Carbon In Liquid Chromatography, Solid-Liquid Extraction, And Capillary Electrophoresis |
| title_short |
Fundamental Studies With Functionalized Low Temperature Glassy Carbon In Liquid Chromatography, Solid-Liquid Extraction, And Capillary Electrophoresis |
| title_full |
Fundamental Studies With Functionalized Low Temperature Glassy Carbon In Liquid Chromatography, Solid-Liquid Extraction, And Capillary Electrophoresis |
| title_fullStr |
Fundamental Studies With Functionalized Low Temperature Glassy Carbon In Liquid Chromatography, Solid-Liquid Extraction, And Capillary Electrophoresis |
| title_full_unstemmed |
Fundamental Studies With Functionalized Low Temperature Glassy Carbon In Liquid Chromatography, Solid-Liquid Extraction, And Capillary Electrophoresis |
| title_sort |
fundamental studies with functionalized low temperature glassy carbon in liquid chromatography, solid-liquid extraction, and capillary electrophoresis |
| publisher |
The Ohio State University / OhioLINK |
| publishDate |
2008 |
| url |
http://rave.ohiolink.edu/etdc/view?acc_num=osu1218477883 |
| work_keys_str_mv |
AT shearerjustinw fundamentalstudieswithfunctionalizedlowtemperatureglassycarboninliquidchromatographysolidliquidextractionandcapillaryelectrophoresis |
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