Nanoscalar modifications to tissue engineering scaffolds: Effect on cellular behavior
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The Ohio State University / OhioLINK
2004
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| Online Access: | http://rave.ohiolink.edu/etdc/view?acc_num=osu1095780106 |
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ndltd-OhioLink-oai-etd.ohiolink.edu-osu10957801062021-08-03T05:49:14Z Nanoscalar modifications to tissue engineering scaffolds: Effect on cellular behavior Powell, Heather Megan tissue engineering polymers nanoscale plasma etching cell cytoskeleton Polymeric scaffolds provide a surface that can facilitate cell growth and tissue morphogenesis. Of particular interest is the role of nanoscalar features on cell behavior. Nanoscale topographies can be generated on two-dimensional polymeric substrates via reactive ion etching. The magnitude and morphology of the resultant surfaces can be tailored by varying the gas media, etching time and power used. Nanofibrillar surfaces were produced on polyethylene terephthalate films via oxygen-plasma etching. These nanofibrils were dimensionally similar to collagen fibers. Cells cultured on nanofibrillar surfaces were shown to have a disrupted cytoskeleton, lower levels of cell-substrate signaling, reduced strength of adhesion and an inhibition of lipid droplet coalescence. The results suggest that cells can detect nanoscalar surface topographies and alter their function in response to these environmental stimuli. While nanofibrillar surfaces can be considered pseudo-three dimensional, they cannot produce 3-D cell structures. Thus truly three dimensional scaffolds must be fabricated to determine the role of nanoscalar fibers on cell organization and function. Electrospinning was employed to generate 3-D meshes of polycaprolactone, a common biodegradable polymer. These nonwoven meshes were comprised of 500 nm fibers with an average pore size of 5 µm. In addition to forming mats of nonwoven fibers, electrospinning technology can also produce tubular scaffolds. These tubular scaffolds were seeded with human vascular smooth muscle cells and cultured for two days. After 2 days in culture, cells assumed a helical orientation around the lumen of the tube, an architecture which closely mimics natural blood vessels. Thus electrospun scaffolds facilitate the growth and organization of cell populations in a manner which imitates the natural tissue. 2004-10-12 English text The Ohio State University / OhioLINK http://rave.ohiolink.edu/etdc/view?acc_num=osu1095780106 http://rave.ohiolink.edu/etdc/view?acc_num=osu1095780106 unrestricted This thesis or dissertation is protected by copyright: all rights reserved. It may not be copied or redistributed beyond the terms of applicable copyright laws. |
| collection |
NDLTD |
| language |
English |
| sources |
NDLTD |
| topic |
tissue engineering polymers nanoscale plasma etching cell cytoskeleton |
| spellingShingle |
tissue engineering polymers nanoscale plasma etching cell cytoskeleton Powell, Heather Megan Nanoscalar modifications to tissue engineering scaffolds: Effect on cellular behavior |
| author |
Powell, Heather Megan |
| author_facet |
Powell, Heather Megan |
| author_sort |
Powell, Heather Megan |
| title |
Nanoscalar modifications to tissue engineering scaffolds: Effect on cellular behavior |
| title_short |
Nanoscalar modifications to tissue engineering scaffolds: Effect on cellular behavior |
| title_full |
Nanoscalar modifications to tissue engineering scaffolds: Effect on cellular behavior |
| title_fullStr |
Nanoscalar modifications to tissue engineering scaffolds: Effect on cellular behavior |
| title_full_unstemmed |
Nanoscalar modifications to tissue engineering scaffolds: Effect on cellular behavior |
| title_sort |
nanoscalar modifications to tissue engineering scaffolds: effect on cellular behavior |
| publisher |
The Ohio State University / OhioLINK |
| publishDate |
2004 |
| url |
http://rave.ohiolink.edu/etdc/view?acc_num=osu1095780106 |
| work_keys_str_mv |
AT powellheathermegan nanoscalarmodificationstotissueengineeringscaffoldseffectoncellularbehavior |
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1719426084331585536 |