Somatic embryogenesis and transformation of cassava for enhanced starch production

Somatic embryogenesis and transformation of cassava for enhanced starch production

Bibliographic Details
Main Author: Ihemere, Uzoma Enyinnaya
Language:English
Published: The Ohio State University / OhioLINK 2003
Subjects:
cassava
ADP-glucose pyrophosphorylase
starch
somatic embryogenesis
transformation
Online Access:http://rave.ohiolink.edu/etdc/view?acc_num=osu1070549008
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spelling ndltd-OhioLink-oai-etd.ohiolink.edu-osu10705490082021-08-03T05:48:25Z Somatic embryogenesis and transformation of cassava for enhanced starch production Ihemere, Uzoma Enyinnaya cassava ADP-glucose pyrophosphorylase starch somatic embryogenesis transformation Cassava (Manihot esculenta Crantz), a member of the family Euphorbiaceae, is one of the most important food crops of sub-Saharan Africa. One of the constraints for cassava starch production is the long growing season. Cassava typically takes 9-12 months to yield a good harvest. This is longer than other major starch-producing crops such as corn and potatoes. We report here the generation of transgenic cassava with increased starch biosynthesis capacity. This was achieved by enhancing the activity of ADP-glucose pyrophosphorylase (AGPase), the rate-limiting enzyme in the starch biosynthesis, in transgenic cassava. To do this, we transformed cassava with a modified E. coli glgC gene encoding AGPase. The glgC gene was modified by site-directed mutagenesis (K296E/G336D) to remove the allosteric regulation (enhancement by fructose1,6-P and inhibition by AMP) sites and to increase the velocity of the enzyme. Root-specific expression of the glgC gene product was achieved using the tuber-specific patatin promoter of potato. We obtained antibiotic-resistant putative transformed plants which have been shown to have integrated and expressed the transgene by PCR, Southern blot, RT-PCR and enzyme activity analyses. AGPase enzyme activity in transformed plants was increased by more than 65%. Significantly, transgenic plants expressing the bacterial glgC gene had two-fold greater top (leaf and stem) and root biomass than wild-type plants grown in the greenhouse. 2003 English text The Ohio State University / OhioLINK http://rave.ohiolink.edu/etdc/view?acc_num=osu1070549008 http://rave.ohiolink.edu/etdc/view?acc_num=osu1070549008 unrestricted This thesis or dissertation is protected by copyright: all rights reserved. It may not be copied or redistributed beyond the terms of applicable copyright laws.
collection NDLTD
language English
sources NDLTD
topic cassava
ADP-glucose pyrophosphorylase
starch
somatic embryogenesis
transformation
spellingShingle cassava
ADP-glucose pyrophosphorylase
starch
somatic embryogenesis
transformation
Ihemere, Uzoma Enyinnaya
Somatic embryogenesis and transformation of cassava for enhanced starch production
author Ihemere, Uzoma Enyinnaya
author_facet Ihemere, Uzoma Enyinnaya
author_sort Ihemere, Uzoma Enyinnaya
title Somatic embryogenesis and transformation of cassava for enhanced starch production
title_short Somatic embryogenesis and transformation of cassava for enhanced starch production
title_full Somatic embryogenesis and transformation of cassava for enhanced starch production
title_fullStr Somatic embryogenesis and transformation of cassava for enhanced starch production
title_full_unstemmed Somatic embryogenesis and transformation of cassava for enhanced starch production
title_sort somatic embryogenesis and transformation of cassava for enhanced starch production
publisher The Ohio State University / OhioLINK
publishDate 2003
url http://rave.ohiolink.edu/etdc/view?acc_num=osu1070549008
work_keys_str_mv AT ihemereuzomaenyinnaya somaticembryogenesisandtransformationofcassavaforenhancedstarchproduction
_version_ 1719425900234145792

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