Genetic and biochemical characterization of resistance to bacterial canker of tomato caused by <i>Clavibacter michiganensis</i> subsp. <i>michiganensis</i>
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The Ohio State University / OhioLINK
2003
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| Online Access: | http://rave.ohiolink.edu/etdc/view?acc_num=osu1069188955 |
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ndltd-OhioLink-oai-etd.ohiolink.edu-osu10691889552021-08-03T05:48:25Z Genetic and biochemical characterization of resistance to bacterial canker of tomato caused by <i>Clavibacter michiganensis</i> subsp. <i>michiganensis</i> Coaker, Gitta Laurel quantitative trait loci marker-assisted selection quantitative disease resistance vascular morphology 2-DE two-dimensional polyacrylamide gel electrophoresis mass spectrometry <p>Bacterial canker of tomato, caused by <i>Clavibacter michiganensis</i> subsp. <i>michiganensis</i> ( <i>Cmm</i> ), is a devastating disease that proliferates in tomato xylem vessels. This research was initiated to genetically and biochemically characterize two quantitative trait loci (QTL) from <i>Lycopersicon hirsutum</i> accession LA407, Rcm2.0 and Rcm5.1, that confer partial resistance to <i>Cmm</i> . Research conducted in this dissertation tested the hypothesis that both loci confer resistance through differing mechanisms.</p> <p>A recurrent selection program was employed during QTL detection to simultaneously advance breeding material while mapping loci and testing genetic hypotheses. The precise genetic location of Rcm2.0 and Rcm5.1 was determined by interval mapping techniques and the analysis of recombinant families. Rcm2.0 was positioned to a 4.4 cM interval on chromosome 2 while Rcm5.1 was positioned to a 2.2 cM interval on chromosome 5. Both loci exhibited additive gene action and interact epistatically.</p> <p>A difference in vascular morphology between resistant LA407 and susceptible cultivated lines was observed. Single marker-QTL analysis revealed that Rcm2.0 and the QTL from LA407 controlling an increased rate of vascular development and maturation both map to the same genetic location. Thus, Rcm2.0 may confer resistance due to a faster maturing and therefore more resistant vascular system.</p> <p>Protein expression was analyzed during <i>Cmm</i> infection using the technique of two-dimensional polyacrylamide gel electrophoresis (2-DE). Forty-one differentially expressed proteins were identified by mass spectral analysis. The accumulation of specific proteins depended upon genotype and time post-inoculation. Rcm2.0 and Rcm5.1 induced the appearance of specific proteins, providing supporting evidence that these loci confer resistance through distinct mechanisms.</p> <p> <i>Cmm</i> cultures were also analyzed to identify <i>Cmm</i> proteins expressed <i>in planta</i> and to gain a greater understanding of <i>Cmm</i> protein expression. Although I was unable to identify <i>Cmm</i> proteins expressed <i>in planta</i> , reproducible differences in protein expression between growth phases was detected. Mass spectral analysis identified 15 out of 16 proteins by analysis of DNA sequences in publicly available genome databases. This illustrates the feasibility of protein identification by analyzing sequence data from closely related organisms. The differentially regulated proteins identified can be pursued in future experiments to determine their role in <i>Cmm</i> virulence.</p> 2003 English text The Ohio State University / OhioLINK http://rave.ohiolink.edu/etdc/view?acc_num=osu1069188955 http://rave.ohiolink.edu/etdc/view?acc_num=osu1069188955 unrestricted This thesis or dissertation is protected by copyright: all rights reserved. It may not be copied or redistributed beyond the terms of applicable copyright laws. |
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NDLTD |
| language |
English |
| sources |
NDLTD |
| topic |
quantitative trait loci marker-assisted selection quantitative disease resistance vascular morphology 2-DE two-dimensional polyacrylamide gel electrophoresis mass spectrometry |
| spellingShingle |
quantitative trait loci marker-assisted selection quantitative disease resistance vascular morphology 2-DE two-dimensional polyacrylamide gel electrophoresis mass spectrometry Coaker, Gitta Laurel Genetic and biochemical characterization of resistance to bacterial canker of tomato caused by <i>Clavibacter michiganensis</i> subsp. <i>michiganensis</i> |
| author |
Coaker, Gitta Laurel |
| author_facet |
Coaker, Gitta Laurel |
| author_sort |
Coaker, Gitta Laurel |
| title |
Genetic and biochemical characterization of resistance to bacterial canker of tomato caused by <i>Clavibacter michiganensis</i> subsp. <i>michiganensis</i> |
| title_short |
Genetic and biochemical characterization of resistance to bacterial canker of tomato caused by <i>Clavibacter michiganensis</i> subsp. <i>michiganensis</i> |
| title_full |
Genetic and biochemical characterization of resistance to bacterial canker of tomato caused by <i>Clavibacter michiganensis</i> subsp. <i>michiganensis</i> |
| title_fullStr |
Genetic and biochemical characterization of resistance to bacterial canker of tomato caused by <i>Clavibacter michiganensis</i> subsp. <i>michiganensis</i> |
| title_full_unstemmed |
Genetic and biochemical characterization of resistance to bacterial canker of tomato caused by <i>Clavibacter michiganensis</i> subsp. <i>michiganensis</i> |
| title_sort |
genetic and biochemical characterization of resistance to bacterial canker of tomato caused by <i>clavibacter michiganensis</i> subsp. <i>michiganensis</i> |
| publisher |
The Ohio State University / OhioLINK |
| publishDate |
2003 |
| url |
http://rave.ohiolink.edu/etdc/view?acc_num=osu1069188955 |
| work_keys_str_mv |
AT coakergittalaurel geneticandbiochemicalcharacterizationofresistancetobacterialcankeroftomatocausedbyiclavibactermichiganensisisubspimichiganensisi |
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1719425872784523264 |