Contusive Spinal Cord Injury: Endogenous Responses of Descending Systems and Effects of Acute Transplantion of Glial Restricted Precursor Cells
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The Ohio State University / OhioLINK
2002
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| Online Access: | http://rave.ohiolink.edu/etdc/view?acc_num=osu1032795301 |
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ndltd-OhioLink-oai-etd.ohiolink.edu-osu10327953012021-08-03T05:47:34Z Contusive Spinal Cord Injury: Endogenous Responses of Descending Systems and Effects of Acute Transplantion of Glial Restricted Precursor Cells Hill, Caitlin E. Spinal cord injury contusion precursor cells GRP cells Glial restricted precursor cells regeneration corticospinal tract reticulospinal tract serotonin proteoglycans CSPG histology gliosis astrocytes anterograde tracer endogenous repair Contusive spinal cord injury (SCI) in the rat mimics many of the aspects of human injury. It provides a good model for examination of endogenous responses to injury, and alterations that occur following therapeutic manipulations. Damage to the spinal cord, results in cell death at the injury site and the development of a chronic cystic lesion cavity, separated into chambers by tissue bridges, and from the spared white matter by a glial and molecular scar. Transplantation of stem cells and immature cells can ameliorate tissue damage, induce axonal regeneration, and improve locomotion. However, unless these cells are pushed down a neuronal lineage, the majority of cells become glia; suggesting that the alterations observed are potentially glially mediated. Glial restricted precursor (GRP) cells-a precursor cell population restricted to oligodendrocyte and astrocyte lineages-offer a novel way to examine the effects of glial cells after injury. The studies performed within, examine the endogenous responses of decending axons from the cortex and brainstem to SCI, and how they respond to acute transplantation of GRP cells. The survival and differentiation of GRP cells, and their ability to modulate the development of the lesion is also examined. GRP cells isolated from a transgenic rat that ubiquitously expresses human placental alkaline phosphatase (hPLAP) were used to specifically detect transplanted cells. Following transplantation, GRP cells retained their differentiation potential. Transplanted GRP cells altered the lesion environment, reducing astrocytic scarring and the expression of inhibitory proteoglycans. After SCI descending systems from the cortex and brainstem initiated endogenous sprouting responses after injury, but these responses were limited and delayed. Transplanted GRP cells supported modest axonal growth from corticospinal tract (CST) and raphespinal axons, and altered the morphology of CST axons towards that of growth cones, suggesting that GRP cells can support axonal growth, however, a longer time course may be required for more extensive growth. The recent isolation and characterization of GRP cells means that many questions remain to be answered about this precursor cell population. The results of this initial qualitative study indicate that GRP cells may be useful in repairing the spinal cord after injury. 2002-10-18 English text The Ohio State University / OhioLINK http://rave.ohiolink.edu/etdc/view?acc_num=osu1032795301 http://rave.ohiolink.edu/etdc/view?acc_num=osu1032795301 unrestricted This thesis or dissertation is protected by copyright: all rights reserved. It may not be copied or redistributed beyond the terms of applicable copyright laws. |
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NDLTD |
| language |
English |
| sources |
NDLTD |
| topic |
Spinal cord injury contusion precursor cells GRP cells Glial restricted precursor cells regeneration corticospinal tract reticulospinal tract serotonin proteoglycans CSPG histology gliosis astrocytes anterograde tracer endogenous repair |
| spellingShingle |
Spinal cord injury contusion precursor cells GRP cells Glial restricted precursor cells regeneration corticospinal tract reticulospinal tract serotonin proteoglycans CSPG histology gliosis astrocytes anterograde tracer endogenous repair Hill, Caitlin E. Contusive Spinal Cord Injury: Endogenous Responses of Descending Systems and Effects of Acute Transplantion of Glial Restricted Precursor Cells |
| author |
Hill, Caitlin E. |
| author_facet |
Hill, Caitlin E. |
| author_sort |
Hill, Caitlin E. |
| title |
Contusive Spinal Cord Injury: Endogenous Responses of Descending Systems and Effects of Acute Transplantion of Glial Restricted Precursor Cells |
| title_short |
Contusive Spinal Cord Injury: Endogenous Responses of Descending Systems and Effects of Acute Transplantion of Glial Restricted Precursor Cells |
| title_full |
Contusive Spinal Cord Injury: Endogenous Responses of Descending Systems and Effects of Acute Transplantion of Glial Restricted Precursor Cells |
| title_fullStr |
Contusive Spinal Cord Injury: Endogenous Responses of Descending Systems and Effects of Acute Transplantion of Glial Restricted Precursor Cells |
| title_full_unstemmed |
Contusive Spinal Cord Injury: Endogenous Responses of Descending Systems and Effects of Acute Transplantion of Glial Restricted Precursor Cells |
| title_sort |
contusive spinal cord injury: endogenous responses of descending systems and effects of acute transplantion of glial restricted precursor cells |
| publisher |
The Ohio State University / OhioLINK |
| publishDate |
2002 |
| url |
http://rave.ohiolink.edu/etdc/view?acc_num=osu1032795301 |
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AT hillcaitline contusivespinalcordinjuryendogenousresponsesofdescendingsystemsandeffectsofacutetransplantionofglialrestrictedprecursorcells |
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