Role of Membrane Asymmetry in Nanoparticle-Erythrocyte Interactions

Role of Membrane Asymmetry in Nanoparticle-Erythrocyte Interactions

Bibliographic Details
Main Author: Bigdelou, Parnian
Language:English
Published: Ohio University / OhioLINK 2020
Subjects:
Biomedical Research
Biomedical Engineering
Biology
Erythrocytes
Eryptosis
Phosphatidylserine
Annexin-V
Lipid Vesicles
Cell Membrane
Silica Nanoparticles
Hemolysis
Flow Cytometry
FRET
Online Access:http://rave.ohiolink.edu/etdc/view?acc_num=ohiou1592343805622053
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spelling ndltd-OhioLink-oai-etd.ohiolink.edu-ohiou15923438056220532021-08-03T07:15:21Z Role of Membrane Asymmetry in Nanoparticle-Erythrocyte Interactions Bigdelou, Parnian Biomedical Research Biomedical Engineering Biology Erythrocytes Eryptosis Phosphatidylserine Annexin-V Lipid Vesicles Cell Membrane Silica Nanoparticles Hemolysis Flow Cytometry FRET Disruption of plasma membrane integrity is a primary mechanism of nanoparticle toxicity in cells. Mechanistic studies on nanoparticle-induced membrane damage have been commonly performed using model membranes with a focus on symmetric bilayers, overlooking the fact that the membrane has an asymmetric phospholipid composition. In this thesis, erythrocytes with normal and scrambled membrane asymmetry were utilized to examine how loss of membrane asymmetry and the resulting alterations in the outer leaflet lipid composition affect nanoparticle-membrane interactions. Loss of membrane asymmetry was achieved by induction of eryptosis, using a calcium ionophore. Erythrocyte membrane disruption (hemolysis) by plain silica nanoparticles was significantly reduced in eryptotic compared to healthy cells. In agreement, a significant reduction in nanoparticle binding to the membrane was observed upon loss of membrane asymmetry. The particles also caused significant cell deformation, changing healthy erythrocytes into a spheroid shape. In agreement with findings in cells, plain particles disrupted lipid vesicles mimicking the erythrocyte outer leaflet lipid composition. The degree of disruption, and nanoparticle binding to the membrane, was reduced in vesicles mimicking the composition of scrambled membranes. Together, findings indicate an important role for the lipid composition of the membrane outer leaflet in nanoparticle-induced membrane damage in both lipid vesicles and erythrocytes. 2020-09-17 English text Ohio University / OhioLINK http://rave.ohiolink.edu/etdc/view?acc_num=ohiou1592343805622053 http://rave.ohiolink.edu/etdc/view?acc_num=ohiou1592343805622053 unrestricted This thesis or dissertation is protected by copyright: some rights reserved. It is licensed for use under a Creative Commons license. Specific terms and permissions are available from this document's record in the OhioLINK ETD Center.
collection NDLTD
language English
sources NDLTD
topic Biomedical Research
Biomedical Engineering
Biology
Erythrocytes
Eryptosis
Phosphatidylserine
Annexin-V
Lipid Vesicles
Cell Membrane
Silica Nanoparticles
Hemolysis
Flow Cytometry
FRET
spellingShingle Biomedical Research
Biomedical Engineering
Biology
Erythrocytes
Eryptosis
Phosphatidylserine
Annexin-V
Lipid Vesicles
Cell Membrane
Silica Nanoparticles
Hemolysis
Flow Cytometry
FRET
Bigdelou, Parnian
Role of Membrane Asymmetry in Nanoparticle-Erythrocyte Interactions
author Bigdelou, Parnian
author_facet Bigdelou, Parnian
author_sort Bigdelou, Parnian
title Role of Membrane Asymmetry in Nanoparticle-Erythrocyte Interactions
title_short Role of Membrane Asymmetry in Nanoparticle-Erythrocyte Interactions
title_full Role of Membrane Asymmetry in Nanoparticle-Erythrocyte Interactions
title_fullStr Role of Membrane Asymmetry in Nanoparticle-Erythrocyte Interactions
title_full_unstemmed Role of Membrane Asymmetry in Nanoparticle-Erythrocyte Interactions
title_sort role of membrane asymmetry in nanoparticle-erythrocyte interactions
publisher Ohio University / OhioLINK
publishDate 2020
url http://rave.ohiolink.edu/etdc/view?acc_num=ohiou1592343805622053
work_keys_str_mv AT bigdelouparnian roleofmembraneasymmetryinnanoparticleerythrocyteinteractions
_version_ 1719457514137845760

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