Mechanism and Mitigation of Biocorrosion by Nitrate Reducing <i>Pseudomonas aeruginosa</i> against Stainless Steel
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Ohio University / OhioLINK
2016
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| Online Access: | http://rave.ohiolink.edu/etdc/view?acc_num=ohiou1479224328510291 |
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ndltd-OhioLink-oai-etd.ohiolink.edu-ohiou14792243285102912021-08-03T06:38:56Z Mechanism and Mitigation of Biocorrosion by Nitrate Reducing <i>Pseudomonas aeruginosa</i> against Stainless Steel Yang, Dongqing Biomedical Engineering Chemical Engineering microbiologically influenced corrosion nitrate reducing bacteria Pseudomonas aeruginosa mitigation stainless steel Biocorrosion, also known as microbiologically influenced corrosion (MIC), is the deterioration of metals and other materials due to the metabolic activity of microorganisms. MIC is a major problem in the oil and gas field and many other industries. It is caused by microbes in biofilms. In addition, biofilms have been recognized to be the cause of infections. It also occurs in medical devices and implants in the human body.Biocatalytic cathodic sulfate reduction (BCSR) theory illustrates the mechanism of MIC caused by sulfate reducing bacteria (SRB) from a bioenergetics perspective. BCSR can be adapted to explain MIC caused by other types of microbes such as nitrate reducing bacteria (NRB) in the form of biocatalytic cathodic nitrate reduction (BCNR). <i>Pseudomonas aeruginosa</i> is known as an opportunistic pathogen, which can grow as NRB using denitrification under anaerobic condition. In this work, the BCNR theory was supported by the starvation test using the wild-type <i>P. aeruginosa</i> (PAO1) in an anaerobic environment. The results suggested that NRB switched to elemental iron (Fe<sup>0</sup>) as an electron donor when there was a shortage of carbon sources under anaerobic condition. Compared to the control test, the highest weight loss (58% increase) and pit depth (more than twice deeper) were achieved under no carbon source condition.Extracellular electron transfer (EET) explains how electrons transfer from steel surface to sessile cells and cause MIC pitting corrosion of NRB. Two common electron mediators, riboflavin and flavin adenine dinucleotide (FAD), were tested to accelerate the electron transfer and enhance MIC in lab tests. The results showed that the maximum pit depth increased about 40% by adding either 10 ppm (w/w) FAD or 10 ppm riboflavin.Biocorrosion can occur in various fields and affect a variety of metal materials. Primarily due to their corrosion resistance, stainless steels have been widely used in the biomedical field. However, stainless steel is not antimicrobial and it may suffer MIC attack. The experimental data in this work showed that MIC pitting corrosion occurred in 304 type stainless steel caused by anaerobic PAO1 culture. A maximum pit depth of 3.91 µm was obtained after the 7-day culture. For the 14-day culture, more pits and larger pit diameters were observed. The maximum pit depth was 7.4 µm.D-amino acids have been investigated as antimicrobial enhancers to trigger biofilm disassembly under an antimicrobial stress. In order to inhibit and kill <i>P. aeruginosa</i>, a cocktail of D-tyrosine (D-tyr) and ciprofloxacin (CIP) was investigated in both the biofilm prevention test and the biofilm removal test using anaerobic PAO1 biofilms grown on carbon steel. The combined effect showed less weight loss and lower pit depth in the prevention test and achieved lower sessile cell counts in both the biofilm prevention test and the biofilm removal test. Compared to 30 ppm CIP alone treatment, there were one extra log reduction in the sessile cell count, an extra 24% reduction in the weight loss, an extra 17% decrease in the average maximum pit depth by using the cocktail of 30 ppm CIP + 2 ppm D-tyr in the biofilm prevention test. 2016 English text Ohio University / OhioLINK http://rave.ohiolink.edu/etdc/view?acc_num=ohiou1479224328510291 http://rave.ohiolink.edu/etdc/view?acc_num=ohiou1479224328510291 unrestricted This thesis or dissertation is protected by copyright: all rights reserved. It may not be copied or redistributed beyond the terms of applicable copyright laws. |
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NDLTD |
| language |
English |
| sources |
NDLTD |
| topic |
Biomedical Engineering Chemical Engineering microbiologically influenced corrosion nitrate reducing bacteria Pseudomonas aeruginosa mitigation stainless steel |
| spellingShingle |
Biomedical Engineering Chemical Engineering microbiologically influenced corrosion nitrate reducing bacteria Pseudomonas aeruginosa mitigation stainless steel Yang, Dongqing Mechanism and Mitigation of Biocorrosion by Nitrate Reducing <i>Pseudomonas aeruginosa</i> against Stainless Steel |
| author |
Yang, Dongqing |
| author_facet |
Yang, Dongqing |
| author_sort |
Yang, Dongqing |
| title |
Mechanism and Mitigation of Biocorrosion by Nitrate Reducing <i>Pseudomonas aeruginosa</i> against Stainless Steel |
| title_short |
Mechanism and Mitigation of Biocorrosion by Nitrate Reducing <i>Pseudomonas aeruginosa</i> against Stainless Steel |
| title_full |
Mechanism and Mitigation of Biocorrosion by Nitrate Reducing <i>Pseudomonas aeruginosa</i> against Stainless Steel |
| title_fullStr |
Mechanism and Mitigation of Biocorrosion by Nitrate Reducing <i>Pseudomonas aeruginosa</i> against Stainless Steel |
| title_full_unstemmed |
Mechanism and Mitigation of Biocorrosion by Nitrate Reducing <i>Pseudomonas aeruginosa</i> against Stainless Steel |
| title_sort |
mechanism and mitigation of biocorrosion by nitrate reducing <i>pseudomonas aeruginosa</i> against stainless steel |
| publisher |
Ohio University / OhioLINK |
| publishDate |
2016 |
| url |
http://rave.ohiolink.edu/etdc/view?acc_num=ohiou1479224328510291 |
| work_keys_str_mv |
AT yangdongqing mechanismandmitigationofbiocorrosionbynitratereducingipseudomonasaeruginosaiagainststainlesssteel |
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