Interactions between E-selectin and Galectin-1 via Ligand Intermediaries During Breast Cancer Metastasis
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Ohio University / OhioLINK
2015
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| Online Access: | http://rave.ohiolink.edu/etdc/view?acc_num=ohiou1438346335 |
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ndltd-OhioLink-oai-etd.ohiolink.edu-ohiou14383463352021-08-03T06:32:33Z Interactions between E-selectin and Galectin-1 via Ligand Intermediaries During Breast Cancer Metastasis Reynolds, Nathan M. Biomedical Engineering E-selectin Galectin-1 Breast Cancer Mac-2BP Invasion of other tissues during bloodborne metastasis requires the adhesion of cancer cells to the endothelium by specific fluid shear-dependent receptor-ligand interactions. It is hypothesized that ligands are shared between the endothelial adhesion molecule known as E-selectin and Galectin-1 (Gal-1), both of which are upregulated during inflammation and cancer. Flow chamber analysis of ZR-75-1 breast cancer monolayers and dynamic biochemical tissue analysis (DBTA) of mucinous breast carcinoma revealed a shear-dependent binding interaction between Gal-1hFc treated surfaces and E-selectin coated Protein A beads causing 300% and 142% binding increase of the beads, respectively. Immunocyto- and immunohistochemical analyses showed that Gal-1 and E-selectin fluorescent signals on ZR-75-1 cells and mucinous carcinoma tissues colocalized at approximately 75% for each assay. Immunoprecipitation of Mac-2BP from breast cancer whole cell lysates displayed that Gal-1 and E-selectin share Mac-2BP as a ligand. Finally, widefield FRET analysis of E-selectin and Gal-1 stained ZR-75-1 cells indicated that previous colocalization data may not be due to Gal-1 and E-selectin on the same ligand. This investigation elucidated a shear-dependent interaction between Gal-1 and E-selectin that may be due to similar or shared ligands. 2015-09-17 English text Ohio University / OhioLINK http://rave.ohiolink.edu/etdc/view?acc_num=ohiou1438346335 http://rave.ohiolink.edu/etdc/view?acc_num=ohiou1438346335 unrestricted This thesis or dissertation is protected by copyright: all rights reserved. It may not be copied or redistributed beyond the terms of applicable copyright laws. |
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NDLTD |
| language |
English |
| sources |
NDLTD |
| topic |
Biomedical Engineering E-selectin Galectin-1 Breast Cancer Mac-2BP |
| spellingShingle |
Biomedical Engineering E-selectin Galectin-1 Breast Cancer Mac-2BP Reynolds, Nathan M. Interactions between E-selectin and Galectin-1 via Ligand Intermediaries During Breast Cancer Metastasis |
| author |
Reynolds, Nathan M. |
| author_facet |
Reynolds, Nathan M. |
| author_sort |
Reynolds, Nathan M. |
| title |
Interactions between E-selectin and Galectin-1 via Ligand Intermediaries During Breast Cancer Metastasis |
| title_short |
Interactions between E-selectin and Galectin-1 via Ligand Intermediaries During Breast Cancer Metastasis |
| title_full |
Interactions between E-selectin and Galectin-1 via Ligand Intermediaries During Breast Cancer Metastasis |
| title_fullStr |
Interactions between E-selectin and Galectin-1 via Ligand Intermediaries During Breast Cancer Metastasis |
| title_full_unstemmed |
Interactions between E-selectin and Galectin-1 via Ligand Intermediaries During Breast Cancer Metastasis |
| title_sort |
interactions between e-selectin and galectin-1 via ligand intermediaries during breast cancer metastasis |
| publisher |
Ohio University / OhioLINK |
| publishDate |
2015 |
| url |
http://rave.ohiolink.edu/etdc/view?acc_num=ohiou1438346335 |
| work_keys_str_mv |
AT reynoldsnathanm interactionsbetweeneselectinandgalectin1vialigandintermediariesduringbreastcancermetastasis |
| _version_ |
1719438665225076736 |