Breast Cancer Cells Acquire a Stem-Like Phenotype by TGFß1/EGF Induced Epithelial-Mesenchymal Transition

Breast Cancer Cells Acquire a Stem-Like Phenotype by TGFß1/EGF Induced Epithelial-Mesenchymal Transition

Bibliographic Details
Main Author: Xiong, Chengkai
Language:English
Published: Ohio University / OhioLINK 2013
Subjects:
Biomedical Engineering
Epithelial-Mesenchymal Transition
stem cell
transforming growth factor
Epidermal Growth Factor
breast cancer
Online Access:http://rave.ohiolink.edu/etdc/view?acc_num=ohiou1366239912
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spelling ndltd-OhioLink-oai-etd.ohiolink.edu-ohiou13662399122021-08-03T05:22:06Z Breast Cancer Cells Acquire a Stem-Like Phenotype by TGFß1/EGF Induced Epithelial-Mesenchymal Transition Xiong, Chengkai Biomedical Engineering Epithelial-Mesenchymal Transition stem cell transforming growth factor Epidermal Growth Factor breast cancer Epithelial-Mesenchymal Transition (EMT) is a key developmental program that is often activated during cancer invasion and metastasis. In breast cancer, EMT is associated with cancer stem cell properties including expression of the stem cell antigenic profile, over-expression of transcription factors involved in EMT, adhesion to bone marrow endothelium and increased migratory capabilities. Research suggests that transforming growth factor (TGF)-ß and Epidermal Growth Factor (EGF) induces EMT and allows cancer cells to become more stem-like. We have shown that TGF-ß1 alone can partly increase expression of EMT-related transcription factors in BT-20 and MCF-7 cells. Furthermore, EGF alone can induce a stem cell-like phenotype in BT-20 cells and promote adhesion on human umbilical vein endothelial cells (HUVECs) but cannot promote expression of EMT-related transcription factors in BT-20 cells. A combination of EGF and TGF-ß1 can promote expression of EMT-related transcription factors in BT-20 cells. These results indicate that a combination of EGF and TGF-ß1 can induce EMT and cause acquisition of a stem-like phenotype by human breast cancer cell lines. 2013-06-17 English text Ohio University / OhioLINK http://rave.ohiolink.edu/etdc/view?acc_num=ohiou1366239912 http://rave.ohiolink.edu/etdc/view?acc_num=ohiou1366239912 unrestricted This thesis or dissertation is protected by copyright: all rights reserved. It may not be copied or redistributed beyond the terms of applicable copyright laws.
collection NDLTD
language English
sources NDLTD
topic Biomedical Engineering
Epithelial-Mesenchymal Transition
stem cell
transforming growth factor
Epidermal Growth Factor
breast cancer
spellingShingle Biomedical Engineering
Epithelial-Mesenchymal Transition
stem cell
transforming growth factor
Epidermal Growth Factor
breast cancer
Xiong, Chengkai
Breast Cancer Cells Acquire a Stem-Like Phenotype by TGFß1/EGF Induced Epithelial-Mesenchymal Transition
author Xiong, Chengkai
author_facet Xiong, Chengkai
author_sort Xiong, Chengkai
title Breast Cancer Cells Acquire a Stem-Like Phenotype by TGFß1/EGF Induced Epithelial-Mesenchymal Transition
title_short Breast Cancer Cells Acquire a Stem-Like Phenotype by TGFß1/EGF Induced Epithelial-Mesenchymal Transition
title_full Breast Cancer Cells Acquire a Stem-Like Phenotype by TGFß1/EGF Induced Epithelial-Mesenchymal Transition
title_fullStr Breast Cancer Cells Acquire a Stem-Like Phenotype by TGFß1/EGF Induced Epithelial-Mesenchymal Transition
title_full_unstemmed Breast Cancer Cells Acquire a Stem-Like Phenotype by TGFß1/EGF Induced Epithelial-Mesenchymal Transition
title_sort breast cancer cells acquire a stem-like phenotype by tgfß1/egf induced epithelial-mesenchymal transition
publisher Ohio University / OhioLINK
publishDate 2013
url http://rave.ohiolink.edu/etdc/view?acc_num=ohiou1366239912
work_keys_str_mv AT xiongchengkai breastcancercellsacquireastemlikephenotypebytgfß1egfinducedepithelialmesenchymaltransition
_version_ 1719419092109099008

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