Molecular Epidemiology of Trypanosoma (Herpetosoma) rangeli (Kinetoplastida: Trypanosomatidae) in Ecuador, South America, and Study of the Parasite Cell Invasion Mechanism in vitro

Bibliographic Details
Main Author:	Lascano, Segundo Mauricio
Language:	English
Published:	Ohio University / OhioLINK 2009
Subjects:	Biology Microbiology Molecular Biology Parasitology Trypanosoma rangeli Trypanosoma cruzi molecular epidemiology Ecuador cell invasion immunofluorescence
Online Access:	http://rave.ohiolink.edu/etdc/view?acc_num=ohiou1258469326

id	ndltd-OhioLink-oai-etd.ohiolink.edu-ohiou1258469326
record_format	oai_dc
spelling	ndltd-OhioLink-oai-etd.ohiolink.edu-ohiou12584693262021-08-03T05:46:29Z Molecular Epidemiology of <i>Trypanosoma</i> (<i>Herpetosoma</i>) <i>rangeli</i> (Kinetoplastida: Trypanosomatidae) in Ecuador, South America, and Study of the Parasite Cell Invasion Mechanism <i>in vitro</i> Lascano, Segundo Mauricio Biology Microbiology Molecular Biology Parasitology Trypanosoma rangeli Trypanosoma cruzi molecular epidemiology Ecuador cell invasion immunofluorescence <p><i>Trypanosoma rangeli</i> is a protozoan hemoflagellate able to infect insects of the subfamily Triatominae (Hemiptera: Reduviidae), mammals, and humans in the American continent. Although the human infection by <i>T. rangeli</i> is non-pathogenic, the importance of the study of this parasite resides in the fact that it shares the same vectors and mammal reservoirs with <i>T. cruzi</i>, the pathogenic parasite causative of Chagas disease. This situation commonly results in misdiagnosis of Chagas disease in patients living in areas where the two parasites overlap spatially and temporarily. </p><p>The occurrence of <i>T. rangeli</i> in Ecuador had not been documented prior to this study and only sporadic reports of <i>T. rangeli</i>-like organisms had been published. This study was divided in two sections: the objective of the first was to establish the presence of <i>T. rangeli</i> in Ecuador and to carry out an investigation of the relationship of the parasite with its Triatominae vectors and mammal hosts in two regions of the country. <i>Rhodnius ecuadoriensis</i>, <i>Panstrongylus howardi</i>, and <i>Triatoma carrioni</i> were found naturally infected with <i>T. rangeli</i>. Mixed infections with <i>T. rangeli</i> and <i>T. cruzi</i> were also observed in those vectors. Analysis of host preferences for blood meal revealed that the triatomines analyzed had fed on several species of mammals (common rat, mice, dog, cat, goat, guinea pig, human) and an avian species (chicken). The identification of the blood meal sources can contribute to the understanding of the epidemiology of <i>T. cruzi</i> and <i>T. rangeli</i> transmission cycles. </p><p>The objective of the second part of the study was to investigate the mechanism that <i>T. rangeli</i> uses to invade mammalian cells. Immunofluorescence assays were used for this purpose. The Choachi strain of <i>T. rangeli</i> and BALB/c fibroblasts were chosen to evaluate the cell invasion process. <i>T. rangeli</i> invades cells primarily by a lysosome-dependent fashion similar to that of <i>T. cruzi</i>, although <i>T. rangeli</i> seems to lack the alternative lysosome-independent pathway that has been described for <i>T. cruzi</i>. Cells infected with <i>T. rangeli</i> did not show signs of intracellular division up to 288 hours post-infection and parasitemia in BALB/c mice was transient and declined rapidly overtime.</p> 2009 English text Ohio University / OhioLINK http://rave.ohiolink.edu/etdc/view?acc_num=ohiou1258469326 http://rave.ohiolink.edu/etdc/view?acc_num=ohiou1258469326 unrestricted This thesis or dissertation is protected by copyright: all rights reserved. It may not be copied or redistributed beyond the terms of applicable copyright laws.
collection	NDLTD
language	English
sources	NDLTD
topic	Biology Microbiology Molecular Biology Parasitology Trypanosoma rangeli Trypanosoma cruzi molecular epidemiology Ecuador cell invasion immunofluorescence
spellingShingle	Biology Microbiology Molecular Biology Parasitology Trypanosoma rangeli Trypanosoma cruzi molecular epidemiology Ecuador cell invasion immunofluorescence Lascano, Segundo Mauricio Molecular Epidemiology of <i>Trypanosoma</i> (<i>Herpetosoma</i>) <i>rangeli</i> (Kinetoplastida: Trypanosomatidae) in Ecuador, South America, and Study of the Parasite Cell Invasion Mechanism <i>in vitro</i>
author	Lascano, Segundo Mauricio
author_facet	Lascano, Segundo Mauricio
author_sort	Lascano, Segundo Mauricio
title	Molecular Epidemiology of <i>Trypanosoma</i> (<i>Herpetosoma</i>) <i>rangeli</i> (Kinetoplastida: Trypanosomatidae) in Ecuador, South America, and Study of the Parasite Cell Invasion Mechanism <i>in vitro</i>
title_short	Molecular Epidemiology of <i>Trypanosoma</i> (<i>Herpetosoma</i>) <i>rangeli</i> (Kinetoplastida: Trypanosomatidae) in Ecuador, South America, and Study of the Parasite Cell Invasion Mechanism <i>in vitro</i>
title_full	Molecular Epidemiology of <i>Trypanosoma</i> (<i>Herpetosoma</i>) <i>rangeli</i> (Kinetoplastida: Trypanosomatidae) in Ecuador, South America, and Study of the Parasite Cell Invasion Mechanism <i>in vitro</i>
title_fullStr	Molecular Epidemiology of <i>Trypanosoma</i> (<i>Herpetosoma</i>) <i>rangeli</i> (Kinetoplastida: Trypanosomatidae) in Ecuador, South America, and Study of the Parasite Cell Invasion Mechanism <i>in vitro</i>
title_full_unstemmed	Molecular Epidemiology of <i>Trypanosoma</i> (<i>Herpetosoma</i>) <i>rangeli</i> (Kinetoplastida: Trypanosomatidae) in Ecuador, South America, and Study of the Parasite Cell Invasion Mechanism <i>in vitro</i>
title_sort	molecular epidemiology of <i>trypanosoma</i> (<i>herpetosoma</i>) <i>rangeli</i> (kinetoplastida: trypanosomatidae) in ecuador, south america, and study of the parasite cell invasion mechanism <i>in vitro</i>
publisher	Ohio University / OhioLINK
publishDate	2009
url	http://rave.ohiolink.edu/etdc/view?acc_num=ohiou1258469326
work_keys_str_mv	AT lascanosegundomauricio molecularepidemiologyofitrypanosomaiiherpetosomaiirangeliikinetoplastidatrypanosomatidaeinecuadorsouthamericaandstudyoftheparasitecellinvasionmechanismiinvitroi
_version_	1719425042387828736

Molecular Epidemiology of <i>Trypanosoma</i> (<i>Herpetosoma</i>) <i>rangeli</i> (Kinetoplastida: Trypanosomatidae) in Ecuador, South America, and Study of the Parasite Cell Invasion Mechanism <i>in vitro</i>

Similar Items