MICRO-RHEOLOGICAL ASSESSMENT OF NEUTROPHIL MECHANICAL PROPERTIES FOLLOWING ADHESION IN A MODEL CAPILLARY

MICRO-RHEOLOGICAL ASSESSMENT OF NEUTROPHIL MECHANICAL PROPERTIES FOLLOWING ADHESION IN A MODEL CAPILLARY

Bibliographic Details
Main Author: Pai, Anand S.
Language:English
Published: Ohio University / OhioLINK 2006
Subjects:
Micro-rheological
Neutrophil
Mechanical Properties
Adhesion
Online Access:http://rave.ohiolink.edu/etdc/view?acc_num=ohiou1156386677
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spelling ndltd-OhioLink-oai-etd.ohiolink.edu-ohiou11563866772021-08-03T05:43:40Z MICRO-RHEOLOGICAL ASSESSMENT OF NEUTROPHIL MECHANICAL PROPERTIES FOLLOWING ADHESION IN A MODEL CAPILLARY Pai, Anand S. Micro-rheological Neutrophil Mechanical Properties Adhesion <p>The multistep paradigm (tethering, rolling and firm adhesion) for leukocyte adhesion in post-capillary venules has been shown to involve adhesion molecules. There has been considerable debate, however, on the relative importance of biochemical stimuli and mechanical deformation in neutrophil adhesion in lung capillaries, a process observed following bacterial infection in the body. In contrast to venules, where the leukocyte diameter (6-9 µm) is smaller than the diameter of the vessel, in lung capillaries the leukocyte diameter is larger than the capillary diameter (2 to 8 µm). In this study a micropipette aspiration assay developed to act as a flow chamber for the capillaries in the lung is described, wherein the effects of adhesion molecules (such as ICAM-1) over control (BSA) can be observed while different micropipette diameters are used to vary the extent of mechanical deformation. The ‘micro-rheology’ technique of tracking thermal (Brownian) motion of endogenous granules within the neutrophils using DIC video microscopy is used to extract the local intracellular viscoelastic (bulk) moduli. The results indicate regional differences in rheology with a significantly stiffer body region over the end caps which do not differ significantly in stiffness from each other. While the inclusion of adhesion molecules with deformation preserved the regional differences, an increase in viscoelastic moduli (stiffness) was seen. With the requirement for neutrophils to stay sequestered during inflammation, the results seem in line with available literature on leukocyte sequestration mechanisms [1] and neutrophil-endothelial cell adhesion effects [2]. The effort provides an insight into the regional rheological effects of deformation and adhesion molecules on neutrophils.</p> 2006-10-06 English text Ohio University / OhioLINK http://rave.ohiolink.edu/etdc/view?acc_num=ohiou1156386677 http://rave.ohiolink.edu/etdc/view?acc_num=ohiou1156386677 unrestricted This thesis or dissertation is protected by copyright: all rights reserved. It may not be copied or redistributed beyond the terms of applicable copyright laws.
collection NDLTD
language English
sources NDLTD
topic Micro-rheological
Neutrophil
Mechanical Properties
Adhesion
spellingShingle Micro-rheological
Neutrophil
Mechanical Properties
Adhesion
Pai, Anand S.
MICRO-RHEOLOGICAL ASSESSMENT OF NEUTROPHIL MECHANICAL PROPERTIES FOLLOWING ADHESION IN A MODEL CAPILLARY
author Pai, Anand S.
author_facet Pai, Anand S.
author_sort Pai, Anand S.
title MICRO-RHEOLOGICAL ASSESSMENT OF NEUTROPHIL MECHANICAL PROPERTIES FOLLOWING ADHESION IN A MODEL CAPILLARY
title_short MICRO-RHEOLOGICAL ASSESSMENT OF NEUTROPHIL MECHANICAL PROPERTIES FOLLOWING ADHESION IN A MODEL CAPILLARY
title_full MICRO-RHEOLOGICAL ASSESSMENT OF NEUTROPHIL MECHANICAL PROPERTIES FOLLOWING ADHESION IN A MODEL CAPILLARY
title_fullStr MICRO-RHEOLOGICAL ASSESSMENT OF NEUTROPHIL MECHANICAL PROPERTIES FOLLOWING ADHESION IN A MODEL CAPILLARY
title_full_unstemmed MICRO-RHEOLOGICAL ASSESSMENT OF NEUTROPHIL MECHANICAL PROPERTIES FOLLOWING ADHESION IN A MODEL CAPILLARY
title_sort micro-rheological assessment of neutrophil mechanical properties following adhesion in a model capillary
publisher Ohio University / OhioLINK
publishDate 2006
url http://rave.ohiolink.edu/etdc/view?acc_num=ohiou1156386677
work_keys_str_mv AT paianands microrheologicalassessmentofneutrophilmechanicalpropertiesfollowingadhesioninamodelcapillary
_version_ 1719424220766666752

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