Determining the Optimal Protocol for Designing a Unique Expression Vector of Focal Adhesion Kinase

Determining the Optimal Protocol for Designing a Unique Expression Vector of Focal Adhesion Kinase

Bibliographic Details
Main Author: Stauble, Erika
Language:English
Published: Ohio Dominican University Honors Theses / OhioLINK 2021
Subjects:
Cellular Biology
Medicine
Molecular Biology
FAK
Focal Adhesion Kinase
metastasis
cancer
restriction enzymes
long-end overhang PCR
Online Access:http://rave.ohiolink.edu/etdc/view?acc_num=oduhonors1620066353118884
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spelling ndltd-OhioLink-oai-etd.ohiolink.edu-oduhonors16200663531188842021-08-03T07:17:27Z Determining the Optimal Protocol for Designing a Unique Expression Vector of Focal Adhesion Kinase Stauble, Erika Cellular Biology Medicine Molecular Biology FAK Focal Adhesion Kinase metastasis cancer restriction enzymes long-end overhang PCR Millions of people around the world are diagnosed with cancer yearly, and the majority of cancer deaths are due to cancers metastasizing. However, there is not a target treatment for cancer metastasis that is consistently efficacious. Therefore, one of the proteins responsible for cancer metastasis needs to be studied to be able to inhibit it. This protein is Focal Adhesion Kinase (FAK). However, the scientific community does not know a lot about the functionality of the FAK protein, which is necessary in order to inhibit it. Therefore, this research focused on isolating the two domains of FAK, using two protocols, so that they can later be inserted into a recipient plasmid for study. The optimal protocol was determined using PCR and gel electrophoresis. Long-end overhang was found to be effective in altering the original FAK sequence to add a restriction cut site and in isolating the two domains that function in the activation of the protein. Now that the domains are isolated, they can be entered into pGEX to study. If the functionality is better known, the protein can be inhibited, which would largely prevent cancer metastasis. 2021-05-05 English text Ohio Dominican University Honors Theses / OhioLINK http://rave.ohiolink.edu/etdc/view?acc_num=oduhonors1620066353118884 http://rave.ohiolink.edu/etdc/view?acc_num=oduhonors1620066353118884 unrestricted This thesis or dissertation is protected by copyright: all rights reserved. It may not be copied or redistributed beyond the terms of applicable copyright laws.
collection NDLTD
language English
sources NDLTD
topic Cellular Biology
Medicine
Molecular Biology
FAK
Focal Adhesion Kinase
metastasis
cancer
restriction enzymes
long-end overhang PCR
spellingShingle Cellular Biology
Medicine
Molecular Biology
FAK
Focal Adhesion Kinase
metastasis
cancer
restriction enzymes
long-end overhang PCR
Stauble, Erika
Determining the Optimal Protocol for Designing a Unique Expression Vector of Focal Adhesion Kinase
author Stauble, Erika
author_facet Stauble, Erika
author_sort Stauble, Erika
title Determining the Optimal Protocol for Designing a Unique Expression Vector of Focal Adhesion Kinase
title_short Determining the Optimal Protocol for Designing a Unique Expression Vector of Focal Adhesion Kinase
title_full Determining the Optimal Protocol for Designing a Unique Expression Vector of Focal Adhesion Kinase
title_fullStr Determining the Optimal Protocol for Designing a Unique Expression Vector of Focal Adhesion Kinase
title_full_unstemmed Determining the Optimal Protocol for Designing a Unique Expression Vector of Focal Adhesion Kinase
title_sort determining the optimal protocol for designing a unique expression vector of focal adhesion kinase
publisher Ohio Dominican University Honors Theses / OhioLINK
publishDate 2021
url http://rave.ohiolink.edu/etdc/view?acc_num=oduhonors1620066353118884
work_keys_str_mv AT staubleerika determiningtheoptimalprotocolfordesigningauniqueexpressionvectoroffocaladhesionkinase
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