Fabrication and Development of a PCL Electrospun Fiber - Keratin Aerogel Scaffold to Mimic Bruch’s Membrane for the Study of Age-related Macular Degeneration
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Miami University / OhioLINK
2017
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| Online Access: | http://rave.ohiolink.edu/etdc/view?acc_num=miami1502103996594191 |
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ndltd-OhioLink-oai-etd.ohiolink.edu-miami15021039965941912021-08-03T07:03:46Z Fabrication and Development of a PCL Electrospun Fiber - Keratin Aerogel Scaffold to Mimic Bruch’s Membrane for the Study of Age-related Macular Degeneration Zeng, Ziqian Biomedical Engineering Electrospinning Keratin aerogel Age-related macular Degeneration Age-related Macular Degeneration (AMD) is a retinal disease responsible for 8.7% of blindness globally, and it is predicted that 196 million people will be affected by the year 2020. Further, there is currently no cure for AMD. Early stage AMD is usually triggered by dysfunction of the retinal pigment epithelium (RPE), and the Bruch’s membrane is often invaded by abnormal angiogenesis around the retina as the disease progress. A biomimetic tissue engineered retinal scaffold may be beneficial in understanding disease etiology and also a possible restorative treatment for AMD. As a primary step toward the development of a tissue engineered retinal scaffold, this thesis focuses on the conceptualization, design, fabrication, and characterization of a scaffold system that combines electrospun polycaprolactone (PCL) fibers to mimic the Bruch’s membrane and keratin aerogel to provide structural support reminiscent of the retinal choroid. Results of this work demonstrate that PCL/keratin fibrous-aerogel shows morphological similarity to the native retinal Bruch’s membrane under SEM, tunable compressive modulus within a range similar to the native retinal extracellular matrix, and minimal toxicity to ARPE-19 cell line. Importantly, the formation of cell monolayers suggests that such system may be suitable as a model system for testing key parameters associated with retinal injury and/or regeneration. 2017-08-11 English text Miami University / OhioLINK http://rave.ohiolink.edu/etdc/view?acc_num=miami1502103996594191 http://rave.ohiolink.edu/etdc/view?acc_num=miami1502103996594191 unrestricted This thesis or dissertation is protected by copyright: some rights reserved. It is licensed for use under a Creative Commons license. Specific terms and permissions are available from this document's record in the OhioLINK ETD Center. |
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NDLTD |
| language |
English |
| sources |
NDLTD |
| topic |
Biomedical Engineering Electrospinning Keratin aerogel Age-related macular Degeneration |
| spellingShingle |
Biomedical Engineering Electrospinning Keratin aerogel Age-related macular Degeneration Zeng, Ziqian Fabrication and Development of a PCL Electrospun Fiber - Keratin Aerogel Scaffold to Mimic Bruch’s Membrane for the Study of Age-related Macular Degeneration |
| author |
Zeng, Ziqian |
| author_facet |
Zeng, Ziqian |
| author_sort |
Zeng, Ziqian |
| title |
Fabrication and Development of a PCL Electrospun Fiber - Keratin Aerogel Scaffold to Mimic Bruch’s Membrane for the Study of Age-related Macular Degeneration |
| title_short |
Fabrication and Development of a PCL Electrospun Fiber - Keratin Aerogel Scaffold to Mimic Bruch’s Membrane for the Study of Age-related Macular Degeneration |
| title_full |
Fabrication and Development of a PCL Electrospun Fiber - Keratin Aerogel Scaffold to Mimic Bruch’s Membrane for the Study of Age-related Macular Degeneration |
| title_fullStr |
Fabrication and Development of a PCL Electrospun Fiber - Keratin Aerogel Scaffold to Mimic Bruch’s Membrane for the Study of Age-related Macular Degeneration |
| title_full_unstemmed |
Fabrication and Development of a PCL Electrospun Fiber - Keratin Aerogel Scaffold to Mimic Bruch’s Membrane for the Study of Age-related Macular Degeneration |
| title_sort |
fabrication and development of a pcl electrospun fiber - keratin aerogel scaffold to mimic bruch’s membrane for the study of age-related macular degeneration |
| publisher |
Miami University / OhioLINK |
| publishDate |
2017 |
| url |
http://rave.ohiolink.edu/etdc/view?acc_num=miami1502103996594191 |
| work_keys_str_mv |
AT zengziqian fabricationanddevelopmentofapclelectrospunfiberkeratinaerogelscaffoldtomimicbruchsmembraneforthestudyofagerelatedmaculardegeneration |
| _version_ |
1719452739513090048 |