STUDY OF PRESSURE DEPENDENCE OF MOLECULAR CONFORMATION OF NADH USING SPECTRAL PHASOR ANALYSIS

Bibliographic Details
Main Author:	Risal, Laxmi
Language:	English
Published:	Miami University / OhioLINK 2016
Subjects:	Biophysics
Online Access:	http://rave.ohiolink.edu/etdc/view?acc_num=miami1469015215

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spelling	ndltd-OhioLink-oai-etd.ohiolink.edu-miami14690152152021-08-03T06:37:35Z STUDY OF PRESSURE DEPENDENCE OF MOLECULAR CONFORMATION OF NADH USING SPECTRAL PHASOR ANALYSIS Risal, Laxmi Biophysics Reduced nicotinamide adenine dinucleotide (NADH) plays an important role in cellular metabolism, serving as an electron carrier in energy-related metabolic pathways. NADH conformational state (whether it is folded or unfolded) has physiological and biophysical significance because NADH is largely folded in solution and unfolded when protein bound. We use spectral phasor analysis technique to assess the thermodynamics and the two state behavior of NADH conformation under high hydrostatic pressure. In order to separate pressure-induced spectral changes due to a conformational change in solvent coupling, we applied a two-state solvent denaturation model, allowing us to estimate the thermodynamic volume change of the folding-unfolding transition. We calculate the volume change of 11.56ml/mol associated with NADH unfolding. In addition to that, we further assess this two-state assumption using a spectral phasor analysis technique, finding no evidence that the two-state behavior of NADH is deviating under high hydrostatic pressure. 2016-08-04 English text Miami University / OhioLINK http://rave.ohiolink.edu/etdc/view?acc_num=miami1469015215 http://rave.ohiolink.edu/etdc/view?acc_num=miami1469015215 unrestricted This thesis or dissertation is protected by copyright: all rights reserved. It may not be copied or redistributed beyond the terms of applicable copyright laws.
collection	NDLTD
language	English
sources	NDLTD
topic	Biophysics
spellingShingle	Biophysics Risal, Laxmi STUDY OF PRESSURE DEPENDENCE OF MOLECULAR CONFORMATION OF NADH USING SPECTRAL PHASOR ANALYSIS
author	Risal, Laxmi
author_facet	Risal, Laxmi
author_sort	Risal, Laxmi
title	STUDY OF PRESSURE DEPENDENCE OF MOLECULAR CONFORMATION OF NADH USING SPECTRAL PHASOR ANALYSIS
title_short	STUDY OF PRESSURE DEPENDENCE OF MOLECULAR CONFORMATION OF NADH USING SPECTRAL PHASOR ANALYSIS
title_full	STUDY OF PRESSURE DEPENDENCE OF MOLECULAR CONFORMATION OF NADH USING SPECTRAL PHASOR ANALYSIS
title_fullStr	STUDY OF PRESSURE DEPENDENCE OF MOLECULAR CONFORMATION OF NADH USING SPECTRAL PHASOR ANALYSIS
title_full_unstemmed	STUDY OF PRESSURE DEPENDENCE OF MOLECULAR CONFORMATION OF NADH USING SPECTRAL PHASOR ANALYSIS
title_sort	study of pressure dependence of molecular conformation of nadh using spectral phasor analysis
publisher	Miami University / OhioLINK
publishDate	2016
url	http://rave.ohiolink.edu/etdc/view?acc_num=miami1469015215
work_keys_str_mv	AT risallaxmi studyofpressuredependenceofmolecularconformationofnadhusingspectralphasoranalysis
_version_	1719440422198050816

STUDY OF PRESSURE DEPENDENCE OF MOLECULAR CONFORMATION OF NADH USING SPECTRAL PHASOR ANALYSIS

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