STRUCTURAL AND TOPOLOGICAL CHARACTERIZATION OF KCNE1 ELUCIDATED BY ELECTRON PARAMAGNETIC RESONANCE SPECTROSCOPYKCNE1

STRUCTURAL AND TOPOLOGICAL CHARACTERIZATION OF KCNE1 ELUCIDATED BY ELECTRON PARAMAGNETIC RESONANCE SPECTROSCOPYKCNE1

Bibliographic Details
Main Author: Gibson, Kaylee Roy
Language:English
Published: Miami University / OhioLINK 2013
Subjects:
Chemistry
Biophysics
Biochemistry
EPR
KCNE1
ESEEM
Power Saturation
Membrane Proteins
Lipid Vesicles
Online Access:http://rave.ohiolink.edu/etdc/view?acc_num=miami1368099139
id ndltd-OhioLink-oai-etd.ohiolink.edu-miami1368099139
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spelling ndltd-OhioLink-oai-etd.ohiolink.edu-miami13680991392021-08-03T05:23:42Z STRUCTURAL AND TOPOLOGICAL CHARACTERIZATION OF KCNE1 ELUCIDATED BY ELECTRON PARAMAGNETIC RESONANCE SPECTROSCOPYKCNE1 Gibson, Kaylee Roy Chemistry Biophysics Biochemistry EPR KCNE1 ESEEM Power Saturation Biochemistry Membrane Proteins Lipid Vesicles KCNE1 (minK), a membrane protein known to modulate the function of the KCNQ1 voltage-gated potassium ion channel in the human heart, was successfully overexpressed, purified into detergent micelles, labeled, and incorporated into POPC:POPG lipid bilayers. CW-EPR and CW-EPR Power Saturation spectroscopy has been used to examine the structural properties of KCNE1 in lipid bilayer vesicles, as opposed to detergent micelles. Preliminary data was also collected for the development of an ESEEM EPR technique which will allow for quick determination of the local secondary structure of membrane proteins in lipid bilayers as opposed to micelles to better model the native membrane environment. 2013-05-10 English text Miami University / OhioLINK http://rave.ohiolink.edu/etdc/view?acc_num=miami1368099139 http://rave.ohiolink.edu/etdc/view?acc_num=miami1368099139 unrestricted This thesis or dissertation is protected by copyright: all rights reserved. It may not be copied or redistributed beyond the terms of applicable copyright laws.
collection NDLTD
language English
sources NDLTD
topic Chemistry
Biophysics
Biochemistry
EPR
KCNE1
ESEEM
Power Saturation
Biochemistry
Membrane Proteins
Lipid Vesicles
spellingShingle Chemistry
Biophysics
Biochemistry
EPR
KCNE1
ESEEM
Power Saturation
Biochemistry
Membrane Proteins
Lipid Vesicles
Gibson, Kaylee Roy
STRUCTURAL AND TOPOLOGICAL CHARACTERIZATION OF KCNE1 ELUCIDATED BY ELECTRON PARAMAGNETIC RESONANCE SPECTROSCOPYKCNE1
author Gibson, Kaylee Roy
author_facet Gibson, Kaylee Roy
author_sort Gibson, Kaylee Roy
title STRUCTURAL AND TOPOLOGICAL CHARACTERIZATION OF KCNE1 ELUCIDATED BY ELECTRON PARAMAGNETIC RESONANCE SPECTROSCOPYKCNE1
title_short STRUCTURAL AND TOPOLOGICAL CHARACTERIZATION OF KCNE1 ELUCIDATED BY ELECTRON PARAMAGNETIC RESONANCE SPECTROSCOPYKCNE1
title_full STRUCTURAL AND TOPOLOGICAL CHARACTERIZATION OF KCNE1 ELUCIDATED BY ELECTRON PARAMAGNETIC RESONANCE SPECTROSCOPYKCNE1
title_fullStr STRUCTURAL AND TOPOLOGICAL CHARACTERIZATION OF KCNE1 ELUCIDATED BY ELECTRON PARAMAGNETIC RESONANCE SPECTROSCOPYKCNE1
title_full_unstemmed STRUCTURAL AND TOPOLOGICAL CHARACTERIZATION OF KCNE1 ELUCIDATED BY ELECTRON PARAMAGNETIC RESONANCE SPECTROSCOPYKCNE1
title_sort structural and topological characterization of kcne1 elucidated by electron paramagnetic resonance spectroscopykcne1
publisher Miami University / OhioLINK
publishDate 2013
url http://rave.ohiolink.edu/etdc/view?acc_num=miami1368099139
work_keys_str_mv AT gibsonkayleeroy structuralandtopologicalcharacterizationofkcne1elucidatedbyelectronparamagneticresonancespectroscopykcne1
_version_ 1719419753988096000

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